Cell Adhesion

Front 1

composition of gap jn?

Back 1

6 connexins make a connexon, 2 connexons from adjacent cells align to make a gap jn

Front 2

gap jns in plants are called?

Back 2

plasmodesmata

Front 3

gap jn pore size?

Back 3

1.5nm

Front 4

types of occluding jns?

Back 4

tight and septate junctions

Front 5

occluding jn fn?

Back 5

maintain polarity of epithelial cell and restrict diffusion of components; separates fluid on apical side from fluid on basal side

Front 6

gap jn fn?

Back 6

allows exchange of ions an small molecules b/w cytplasm of adjacent cells

Front 7

do gap jns always stay open?

Back 7

No

Front 8

2 transport mechanisms involving tight jns?

Back 8

transcellular and paracellular transport

Front 9

connexin description

Back 9

4 pass transmmb protein

Front 10

which domains of occludin and claudin mediate tight jn?

Back 10

EC domains (also JAM)

Front 11

What makes tight jns?

Back 11

sealing strands: consists of transmmb adhesion proteins occludin and claudin whose EC domains adhere to each other and occlude IC space

Front 12

anchoring protein fn?

Back 12

provide physical support to cell

Front 13

2 classes of anchoring proteins?

Back 13

IC anchor proteins
transmmb adhesion proteins

Front 14

2 types of anchoring jns?

Back 14

cell to cell
cell to matrix

Front 15

2 cell to cell anchoring jns?

Back 15

adherens and desmosomes

Front 16

2 cell to matrix anchoring jns?

Back 16

focal adhesion and hemidesmosomes

Front 17

Which transmmb adhesion protein is involved with cell to cell contact? cell to matrix contact?

Back 17

cadherins (cell to cell)
integrins (cell to matrix)

Front 18

What is adherens jn?

Back 18

connecting bundles of AF from cell to cell; i.e. serve as anchoring site for AFs

Front 19

what is common b/w desmo and hemidesmosomes?

Back 19

both are anchor sites for IF

Front 20

desmosomes are formed from what cadherins?

Back 20

desmosomal cadherins desmoglein and desmocollin

Front 21

what IC anchor proteins are involved in desmosomes?

Back 21

plakoglobin and desmoplakin

Front 22

4 types of anchoring jns?

Back 22

desmosomes
adherens jns
hemidesmosomes
actin-linked cell matrix adhesion

Front 23

which CAMs practice homophilic interactions?

Back 23

cadherins and NCAM

Front 24

which CAMs practice heterophilic interactions?

Back 24

Integrins and Selectins

Front 25

what CAM is found on leukocytes?

Back 25

selectins, mediate vascular cell interactions

Front 26

what domain in selectin binds leukocyte to endothelial cell?

Back 26

Ca2+-dependent lectin domain (a sugar binding domain)

Front 27

lectin domain fn?

Back 27

binds to a specific oligosaccharide on target cell

Front 28

types of selectins?

Back 28

L (on leukocytes)
P (platelets and endothelial cells)
E (endothelial cells)

Front 29

2 types of adhesion in WBC recruitment?

Back 29

weak adhesion and rolling (selectin-dependent) and strong adhesion and emigration (integrin-dependent)

Front 30

what is the only Ca2+-independent CAM group?

Back 30

Ig-Superfamily CAMs

Front 31

how are various isoforms of N-CAM present?

Back 31

alternative splicing of an RNA transcript from a single gene

Front 32

what is similar b/w all N-CAM isoforms?

Back 32

they have 5 EC Ig-like domains

Front 33

describe cadherin

Back 33

single pass transmmb protein with cadherin repeat sequences in the EC domain

Front 34

What [Ca2+] allows cadherin to assume rodlike shape?

Back 34

>1mM

Front 35

Type I cadherins?

Back 35

E-,N-, and P-cadherin

Front 36

Type 2 cadherins?

Back 36

VE-Cadherin

Front 37

where is E-cadherin found?

Back 37

epithelial cells or parts of brain

Front 38

where is N-cadherin found?

Back 38

neural, muscle, and lens cells/fibroblast

Front 39

which cadherin is first expressed in mammalian development?

Back 39

E-cadherin

Front 40

where is p-cadherin found?

Back 40

on placenta and epidermis

Front 41

where is VE-cadherin found?

Back 41

endothelial cells

Front 42

what part of cadherin mediates binding?

Back 42

EC1 domain (N-terminus)

Front 43

what are catenins?

Back 43

IC anchoring proteins that interact with C-terminus of cadherins

Front 44

p120 catenin fn?

Back 44

regulates E-cadherin turnover and stability; regulates strength of adhesion

Front 45

integrin composition

Back 45

2 noncovalently associated transmmb glycoprotein subunits alpha and beta

Front 46

what do EC domains of integrin bind with?

Back 46

fibronectins and laminin

Front 47

what is inside-out signalling?

Back 47

cellular stimulation induces conformation change in talin that exposes talin head domain

Front 48

FAK (Focal adhesion kinase) fn

Back 48

allows communication b/w ECM and inside of the cell

Front 49

focal adhesion protein fn?

Back 49

establishing and maintaining the integrin-cytoskeleton linkage

Front 50

e.g. of focal adhesion proteins?

Back 50

talin, FAK, vinculin

Front 51

FAK fn?

Back 51

required to stabilize linkage b/w focal adhesions and AF

Front 52

what is aggrecan?

Back 52

predominant protein in cartilage

Front 53

heparin fn?

Back 53

used as anticlotting drug

Front 54

how can Talin activate?

Back 54

via G protein coupled receptors and receptor tyrosine kinases which activate IC signalling molecule PIP2, which is thought to activate Talin