Study your flashcards anywhere!

Download the official Cram app for free >

  • Shuffle
    Toggle On
    Toggle Off
  • Alphabetize
    Toggle On
    Toggle Off
  • Front First
    Toggle On
    Toggle Off
  • Both Sides
    Toggle On
    Toggle Off
  • Read
    Toggle On
    Toggle Off

How to study your flashcards.

Right/Left arrow keys: Navigate between flashcards.right arrow keyleft arrow key

Up/Down arrow keys: Flip the card between the front and back.down keyup key

H key: Show hint (3rd side).h key

A key: Read text to speech.a key


Play button


Play button




Click to flip

54 Cards in this Set

  • Front
  • Back
The most commonly used media to culture bacteria in the laboratory is_____
nutrient media
Nutrient media is easily prepared by adding ____ extract and peptone (amino acids) to distilled water.
beef cell
______media are those which contain most of the nutrients that cells need to grow and reproduce.
_________ media on the other hand, is made by adding specific nutrients in exact quantities to distilled water.
chemically defined
Nutrient media can be used as a liquid in which case it is called_________
______are of interest to microbiologists because they include most human pathogens and symbionts.
chemoheterotrophic bacteria
Clinical specimens usually contain a mixture of________
bacterial species
Bacteriologists will start the identification of bacteria by using a group of _______ media.
primary identifying
________media contain additional nutrients that are added to a minimum essential media (MEM) such as nutrient agar.
______heterotrophs are "picky eaters" and will not grow very well in the laboratory unless the specific nutrients are added to media.
The type of enriched media we will use today is called______ agar.
_________media contains specific chemicals that are added to MEM.
The types of selective media we will use to day include Phenyl Ethyl Alcohol and ____________agar.
mannitol salt
Phenyl Ethyl Alcohol will be used to select_________ bacteria that may be found in a patient's specimen.
gram positive
Mannitol salts agar will be used to select a specific genera of ______ bacteria.
gram positive
_____media contains specific indicator chemicals which are added to MEM.
The types of differential media that we will use today include Eosin-Methylene Blue agar and _______agar`
Eosin-Methylene Blue agar can be used to detect the presence of ______ that may be found in a patient's specimen.
escherichia coli
Colonies of Escherichia coli can be recognized because of the _____ sheen they posses when grown on EMB.
metallic green
MacConkey's agar contains the dye_________and bile salts which are substances that inhibits the growth of Gram positive bacteria.
crystal violet
________and the dye, neutral red( red when acidic) are indicators of bacterial fermentation.
Most______ do not produce acid from lactose fermentation, and therefore their colonies appear pink instead of red.
intestinal pathogens
Nutrients that are absorbed by bacteria can be used in a variety of different types of _________ reactions.
When nutrients are used to build more complex molecules in a cell the process is called_______.
If absorbed nutrients are used as a source of energy to fuel the activities of a cell the process is called_________.
During aerobic cellular respiration sugars such as _______ are oxidized completely to CO2 and H2O
As the oxidation of sugars proceeds, energy is released from the ________ that held the atoms together in the sugar.
Fermentation is a ____ biochemical process that certain bacteria can use for the modification of nutrients.
Fermentation is an anaerobic biochemical pathway that requires certain enzymes and the anbsence of ________, a component of air.
molecular oxygen
During fermentation,_______is converted into a number of different products including lactic acid and ethanol.
pyruvic acid
Fermentation may also result in the production of certain gases including_________.
carbon dioxide
________tubes (fermentation) contain a medium made of nnutrient broth, a sugar, and pH indicator.
A_____color in these tubes indicates a lack of fermentation of the sugar but use of other components in the medium.
A_______color in these tubes indicates that the fermentation of the sugar with the production of acid has occurred.
A______in the small inner tube after the incubation period indicates the production of gas.
gas bubble
Each of these traits are helpful in the___________of certain bacteria.
The IMVIC test is used to identify_____ rods that are suspected of being Enterics (intestinal bacteria).
gram negative
The IMVIC test consists of four separate parts- The Indole Test, The voges-proskaur test, the _____test, and the citrate utilization test.
methyl red
Each species in the family of bacteria is called_________ gives a characteristic response to the four IMVIC tests.
Among the bacteria that are called enterics are Escherichia coli and ___________ species (normal intestinal organisms).
One section of the IMVIC tests measures the production of indoles from the amino acid, ________.
Which IMVIC test measures the oxidation of glucose with the production of several acids?______
methyl red
One species that does produce sufficient_______ to change a pH indicator in the Methyl Red Test is Escherichia Coli.
Organisms which do not produce organic acids often produce_________.
2,3 butanediol
Not all organisms are able to ferment citrate as their sole______source.
The ability of an organism to use citrate is dependent on the presence of citrate______ which facilitates the transport of citrate into the cell.
The TSI test is a quick way to determine the presence of _________.
All of the bacteria in question # 1 are gram__________ organisms which ferment glucose.
The TSI test determines the ability of bacteria in this group to also ferment_______ and/or sucrose and to produce Hydrogen sulfide (H2S)
___________ and ferrous sulfate serve as indicators of acid and hydrogen sulfide (H2S) production respectively.
phenol red
When glucose is fermented by an organism the entire tube becmoes________(acidic).
Bacteria metabolizing aerobically, oxidize peptones ( short chains of amino acids) in the medium to ______ amines (red).
The production of ________ will occur in the acidic and anaerobic stab part of the tube if bacteria are capable of this reaction.
hydrogen sulfide
____ color develops when ferric sulfide is produces during this reaction.