Ccp Microbiology

Front 1

The most commonly used media to culture bacteria in the laboratory is_____

Back 1

nutrient media

Front 2

Nutrient media is easily prepared by adding ____ extract and peptone (amino acids) to distilled water.

Back 2

beef cell

Front 3

______media are those which contain most of the nutrients that cells need to grow and reproduce.

Back 3

complex

Front 4

_________ media on the other hand, is made by adding specific nutrients in exact quantities to distilled water.

Back 4

chemically defined

Front 5

Nutrient media can be used as a liquid in which case it is called_________

Back 5

broth

Front 6

______are of interest to microbiologists because they include most human pathogens and symbionts.

Back 6

chemoheterotrophic bacteria

Front 7

Clinical specimens usually contain a mixture of________

Back 7

bacterial species

Front 8

Bacteriologists will start the identification of bacteria by using a group of _______ media.

Back 8

primary identifying

Front 9

________media contain additional nutrients that are added to a minimum essential media (MEM) such as nutrient agar.

Back 9

enriched

Front 10

______heterotrophs are "picky eaters" and will not grow very well in the laboratory unless the specific nutrients are added to media.

Back 10

fastidious

Front 11

The type of enriched media we will use today is called______ agar.

Back 11

blood

Front 12

_________media contains specific chemicals that are added to MEM.

Back 12

selective

Front 13

The types of selective media we will use to day include Phenyl Ethyl Alcohol and ____________agar.

Back 13

mannitol salt

Front 14

Phenyl Ethyl Alcohol will be used to select_________ bacteria that may be found in a patient's specimen.

Back 14

gram positive

Front 15

Mannitol salts agar will be used to select a specific genera of ______ bacteria.

Back 15

gram positive

Front 16

_____media contains specific indicator chemicals which are added to MEM.

Back 16

differential

Front 17

The types of differential media that we will use today include Eosin-Methylene Blue agar and _______agar`

Back 17

MacConkey's

Front 18

Eosin-Methylene Blue agar can be used to detect the presence of ______ that may be found in a patient's specimen.

Back 18

escherichia coli

Front 19

Colonies of Escherichia coli can be recognized because of the _____ sheen they posses when grown on EMB.

Back 19

metallic green

Front 20

MacConkey's agar contains the dye_________and bile salts which are substances that inhibits the growth of Gram positive bacteria.

Back 20

crystal violet

Front 21

________and the dye, neutral red( red when acidic) are indicators of bacterial fermentation.

Back 21

lactose

Front 22

Most______ do not produce acid from lactose fermentation, and therefore their colonies appear pink instead of red.

Back 22

intestinal pathogens

Front 23

Nutrients that are absorbed by bacteria can be used in a variety of different types of _________ reactions.

Back 23

biochemical

Front 24

When nutrients are used to build more complex molecules in a cell the process is called_______.

Back 24

anabolism

Front 25

If absorbed nutrients are used as a source of energy to fuel the activities of a cell the process is called_________.

Back 25

catabolism

Front 26

During aerobic cellular respiration sugars such as _______ are oxidized completely to CO2 and H2O

Back 26

glucose

Front 27

As the oxidation of sugars proceeds, energy is released from the ________ that held the atoms together in the sugar.

Back 27

bonds

Front 28

Fermentation is a ____ biochemical process that certain bacteria can use for the modification of nutrients.

Back 28

catabolic

Front 29

Fermentation is an anaerobic biochemical pathway that requires certain enzymes and the anbsence of ________, a component of air.

Back 29

molecular oxygen

Front 30

During fermentation,_______is converted into a number of different products including lactic acid and ethanol.

Back 30

pyruvic acid

Front 31

Fermentation may also result in the production of certain gases including_________.

Back 31

carbon dioxide

Front 32

________tubes (fermentation) contain a medium made of nnutrient broth, a sugar, and pH indicator.

Back 32

Durham

Front 33

A_____color in these tubes indicates a lack of fermentation of the sugar but use of other components in the medium.

Back 33

red

Front 34

A_______color in these tubes indicates that the fermentation of the sugar with the production of acid has occurred.

Back 34

yellow

Front 35

A______in the small inner tube after the incubation period indicates the production of gas.

Back 35

gas bubble

Front 36

Each of these traits are helpful in the___________of certain bacteria.

Back 36

identification

Front 37

The IMVIC test is used to identify_____ rods that are suspected of being Enterics (intestinal bacteria).

Back 37

gram negative

Front 38

The IMVIC test consists of four separate parts- The Indole Test, The voges-proskaur test, the _____test, and the citrate utilization test.

Back 38

methyl red

Front 39

Each species in the family of bacteria is called_________ gives a characteristic response to the four IMVIC tests.

Back 39

Enterobacteriaceae

Front 40

Among the bacteria that are called enterics are Escherichia coli and ___________ species (normal intestinal organisms).

Back 40

enterobacter

Front 41

One section of the IMVIC tests measures the production of indoles from the amino acid, ________.

Back 41

tryptophan

Front 42

Which IMVIC test measures the oxidation of glucose with the production of several acids?______

Back 42

methyl red

Front 43

One species that does produce sufficient_______ to change a pH indicator in the Methyl Red Test is Escherichia Coli.

Back 43

acid

Front 44

Organisms which do not produce organic acids often produce_________.

Back 44

2,3 butanediol

Front 45

Not all organisms are able to ferment citrate as their sole______source.

Back 45

carbon

Front 46

The ability of an organism to use citrate is dependent on the presence of citrate______ which facilitates the transport of citrate into the cell.

Back 46

permease

Front 47

The TSI test is a quick way to determine the presence of _________.

Back 47

Enterobacteriaceae

Front 48

All of the bacteria in question # 1 are gram__________ organisms which ferment glucose.

Back 48

negative

Front 49

The TSI test determines the ability of bacteria in this group to also ferment_______ and/or sucrose and to produce Hydrogen sulfide (H2S)

Back 49

lactose

Front 50

___________ and ferrous sulfate serve as indicators of acid and hydrogen sulfide (H2S) production respectively.

Back 50

phenol red

Front 51

When glucose is fermented by an organism the entire tube becmoes________(acidic).

Back 51

yellow

Front 52

Bacteria metabolizing aerobically, oxidize peptones ( short chains of amino acids) in the medium to ______ amines (red).

Back 52

basic

Front 53

The production of ________ will occur in the acidic and anaerobic stab part of the tube if bacteria are capable of this reaction.

Back 53

hydrogen sulfide

Front 54

____ color develops when ferric sulfide is produces during this reaction.

Back 54

black