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63 Cards in this Set

  • Front
  • Back
What are the three connective tissue fibers?
elastin, reticulin, collagen
Name stains that will show elastin fibers.
-Verhoeff-van Gieson

-aldehyde fuchsin
Name stains that will show collagen fibers.
-Masson trichrome

-Gomori trichrome
Name stains that will show reticulin fibers.
-Gormri trichrome

-Gordon and Sweets

-Wilder and Snook
Name connective tissue cells.
-adipose

-fibroblasts

-mast

-macrophage

-plasma

-mesenchymal
What are the three types of muscle cells?
skeletal, muscle, and cardiac
With both the Masson and Gomori trichrome produces, muscle stains:
red
Which of the following methods best demonstrates elastic tissue?

a) Verhoeff-van Gieson
b) Silver impregnation
c) Gomori trichrome
d) PAS
a) Verhoeff-van Gieson
The first step in most reticulin methods is:
oxidation
The Verhoeff method differentiates with:
excess mordant
van Gieson solution stains collagen:
red
Bouin solution functions in the Masson trichrome stain is a/an:
mordant
The components of the van Gieson solution are:
picric acid and acid fuchsin
Gomori aldehyde fuchsin solution contains:
basic fuchsin, HCl acid, and paraldehyde
As a toning agent, most silver stains use:
gold chloride
The reducing agent in most reticulin stains is:
formaldehyde
Mallory PTAH stain is:
polychromatic
A good control for reticulin stains is:
liver
Toluidine blue is used to demonstrate which cells?
mast cells
The component of basement membranes that is usually demonstrated with special stains is:
carbohydrate
A stain that might be used to demonstrate cirrhosis of the liver is the:
Masson trichrome
In the Masson trichrome stain, Biebrich scarlet is removed from collagen by:
PTAH
Iron hematoxylin, rather than alumunum hematoxylin, is usually used to stain nuclei in trichrome procedures because:
subsequent staining solutions are acidic
The preferred fixative for the Masson tichrome stain is:
Bouin
Silver impregnation stains for reticulin depend on the formation of which of the following chemical groups?

a) quinoid
b) aldehyde
c) carboxyl
d) amino
b) aldehyde
The diamine silver complex is formed by a reaction between silver and:
ammonia
Sections for the demonstration of basement membranes should be cut at:
2um
The oil red O stain requires which of the following sections?

a) paraffin
b) celloidin
c) frozen
d) plastic
c) frozen
The oil red O stain might be used to demonstrate:

a) rhabdomyosarcomas
b) leiomyosarcomas
c) liposarcomas
d) adenocarcinomas
c) liposarcomas
Which of the following methods is an example of physical staining?

a) toluidine blue
b) Verhoeff-van Gieson
c) methenamine silver
d) oil red O
d) oil red O
Fat is chemically fixed and maintained in tissue by:
osmium tetroxide
Plasma cells can be demonstrated with:
methyl green-pyronin
When ferric ammonium sulfate is used in a silver stain for reticulin, it functions as the:
sensitizer
Mallory PTAH solution is ripened for immediate use by:
potassium permanganate
When used in a silver stain for reticulin, phosphomolybdic acid functions as the:
oxidizer
What is the preferred fixative for aldehyde fuchsin stain?
10% NBF
What is the preferred fixative for Gomori trichrome stain?
bouin
What is the preferred fixative for Masson trichrome stain?
bouin
What is the preferred fixative for Oil red O stain?
10% NBF
What is the preferre fixative for paraffin processed fat?
osmium tetroxide
What is the preferred fixative for the PTAH stain?
Zenker solution
What is the preferred fixative for Gomori reticulin stain?
10% NBF
What is the preferred fixative or the Verhoeff-van Gieson stain?
10% NBF or Zenker solution
What procedures commonly use acid fuchsin?
-Masson trichrome

-van Gieson
What procedures commonly use aniline blue?
-Gomori trichrome

-Masson trichrome
What procedures commonly use basic fuchsin?
aldehyde fuchsin
What procedures commonly use Biebrich scarlet?
-Masson trichrome
What procedures commonly use chromotrope 2R?
Gomori trichrome
What procedures commonly use diamine silver?
Gordon and Sweets
What procedures commonly use iodine?
Verhoeff (primary stain)
What procedures commonly use pyronin?
-RNA
What procedures commonly use paraldehyde?
aldehyde fuchsin
The Verhoeff-van Gieson shows both orange collagen and orange muscle. This most likely could be corrected in the future by:
ensuring the picric acid solution is saturated
The Masson trichrome stain shows only a faint grayish-pink staining of the muscle. This could most likely be prevented in the future by:
using fresh acid fuchsin-Biebrich scarlet solution
A black precipitate is noted on a periodic acid-methenamine silver-stained section that has been on the pathologist's desk for about a month. The stain did not have any precipitate originally. This indicates that most likely the:
sodium thiosulfate step may have been omitted
Fat droplets are seen in the tissue spaces of an oil red O-stained section. This most likely resulted from:
mashing on the coverglass to remove air bubbles
Formalin-fixed tissue shows very faded blue staining with the Masson trichrome technique. The most likely explanation is that the sections were:
left too long in the final acetic acid solution
Microscopic examination of a section stained with the Gomori reticulin stain and counterstained with nuclear fast red shows cloudiness of both the section and the slide. This is most likely the result of:
inadequate washing after the nuclear fast red
Granular disposition of the silver is seen on the control stained with the Gordon and Sweets reticulin method. One possible explanation is that:
the reagents may have been old
Black precipitate is seen on sections stained with the Gomori method for reticulin. This is most likely the result of:
overrinsing the slide prior to the formaldehyde
A Masson stain has been requested and the supply of phosphomolybdic acid has been depleted. The best action is to substitute:
phosphotungstic acid
The connective tissue has failed to stain yellow with the Movat pentachrome stain. One possible cause is that the:
saffron solution contains water
The goblet cells in a section of small intestine fail to show any blue staining with the Movat pentachrome stain. This could be the result of:
treating the slides with alkaline alcohol too briefly