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63 Cards in this Set
- Front
- Back
What are the three connective tissue fibers?
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elastin, reticulin, collagen
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Name stains that will show elastin fibers.
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-Verhoeff-van Gieson
-aldehyde fuchsin |
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Name stains that will show collagen fibers.
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-Masson trichrome
-Gomori trichrome |
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Name stains that will show reticulin fibers.
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-Gormri trichrome
-Gordon and Sweets -Wilder and Snook |
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Name connective tissue cells.
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-adipose
-fibroblasts -mast -macrophage -plasma -mesenchymal |
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What are the three types of muscle cells?
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skeletal, muscle, and cardiac
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With both the Masson and Gomori trichrome produces, muscle stains:
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red
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Which of the following methods best demonstrates elastic tissue?
a) Verhoeff-van Gieson b) Silver impregnation c) Gomori trichrome d) PAS |
a) Verhoeff-van Gieson
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The first step in most reticulin methods is:
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oxidation
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The Verhoeff method differentiates with:
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excess mordant
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van Gieson solution stains collagen:
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red
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Bouin solution functions in the Masson trichrome stain is a/an:
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mordant
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The components of the van Gieson solution are:
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picric acid and acid fuchsin
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Gomori aldehyde fuchsin solution contains:
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basic fuchsin, HCl acid, and paraldehyde
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As a toning agent, most silver stains use:
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gold chloride
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The reducing agent in most reticulin stains is:
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formaldehyde
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Mallory PTAH stain is:
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polychromatic
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A good control for reticulin stains is:
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liver
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Toluidine blue is used to demonstrate which cells?
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mast cells
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The component of basement membranes that is usually demonstrated with special stains is:
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carbohydrate
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A stain that might be used to demonstrate cirrhosis of the liver is the:
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Masson trichrome
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In the Masson trichrome stain, Biebrich scarlet is removed from collagen by:
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PTAH
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Iron hematoxylin, rather than alumunum hematoxylin, is usually used to stain nuclei in trichrome procedures because:
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subsequent staining solutions are acidic
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The preferred fixative for the Masson tichrome stain is:
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Bouin
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Silver impregnation stains for reticulin depend on the formation of which of the following chemical groups?
a) quinoid b) aldehyde c) carboxyl d) amino |
b) aldehyde
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The diamine silver complex is formed by a reaction between silver and:
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ammonia
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Sections for the demonstration of basement membranes should be cut at:
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2um
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The oil red O stain requires which of the following sections?
a) paraffin b) celloidin c) frozen d) plastic |
c) frozen
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The oil red O stain might be used to demonstrate:
a) rhabdomyosarcomas b) leiomyosarcomas c) liposarcomas d) adenocarcinomas |
c) liposarcomas
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Which of the following methods is an example of physical staining?
a) toluidine blue b) Verhoeff-van Gieson c) methenamine silver d) oil red O |
d) oil red O
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Fat is chemically fixed and maintained in tissue by:
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osmium tetroxide
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Plasma cells can be demonstrated with:
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methyl green-pyronin
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When ferric ammonium sulfate is used in a silver stain for reticulin, it functions as the:
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sensitizer
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Mallory PTAH solution is ripened for immediate use by:
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potassium permanganate
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When used in a silver stain for reticulin, phosphomolybdic acid functions as the:
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oxidizer
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What is the preferred fixative for aldehyde fuchsin stain?
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10% NBF
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What is the preferred fixative for Gomori trichrome stain?
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bouin
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What is the preferred fixative for Masson trichrome stain?
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bouin
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What is the preferred fixative for Oil red O stain?
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10% NBF
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What is the preferre fixative for paraffin processed fat?
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osmium tetroxide
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What is the preferred fixative for the PTAH stain?
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Zenker solution
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What is the preferred fixative for Gomori reticulin stain?
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10% NBF
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What is the preferred fixative or the Verhoeff-van Gieson stain?
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10% NBF or Zenker solution
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What procedures commonly use acid fuchsin?
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-Masson trichrome
-van Gieson |
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What procedures commonly use aniline blue?
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-Gomori trichrome
-Masson trichrome |
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What procedures commonly use basic fuchsin?
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aldehyde fuchsin
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What procedures commonly use Biebrich scarlet?
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-Masson trichrome
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What procedures commonly use chromotrope 2R?
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Gomori trichrome
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What procedures commonly use diamine silver?
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Gordon and Sweets
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What procedures commonly use iodine?
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Verhoeff (primary stain)
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What procedures commonly use pyronin?
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-RNA
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What procedures commonly use paraldehyde?
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aldehyde fuchsin
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The Verhoeff-van Gieson shows both orange collagen and orange muscle. This most likely could be corrected in the future by:
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ensuring the picric acid solution is saturated
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The Masson trichrome stain shows only a faint grayish-pink staining of the muscle. This could most likely be prevented in the future by:
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using fresh acid fuchsin-Biebrich scarlet solution
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A black precipitate is noted on a periodic acid-methenamine silver-stained section that has been on the pathologist's desk for about a month. The stain did not have any precipitate originally. This indicates that most likely the:
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sodium thiosulfate step may have been omitted
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Fat droplets are seen in the tissue spaces of an oil red O-stained section. This most likely resulted from:
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mashing on the coverglass to remove air bubbles
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Formalin-fixed tissue shows very faded blue staining with the Masson trichrome technique. The most likely explanation is that the sections were:
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left too long in the final acetic acid solution
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Microscopic examination of a section stained with the Gomori reticulin stain and counterstained with nuclear fast red shows cloudiness of both the section and the slide. This is most likely the result of:
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inadequate washing after the nuclear fast red
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Granular disposition of the silver is seen on the control stained with the Gordon and Sweets reticulin method. One possible explanation is that:
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the reagents may have been old
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Black precipitate is seen on sections stained with the Gomori method for reticulin. This is most likely the result of:
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overrinsing the slide prior to the formaldehyde
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A Masson stain has been requested and the supply of phosphomolybdic acid has been depleted. The best action is to substitute:
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phosphotungstic acid
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The connective tissue has failed to stain yellow with the Movat pentachrome stain. One possible cause is that the:
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saffron solution contains water
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The goblet cells in a section of small intestine fail to show any blue staining with the Movat pentachrome stain. This could be the result of:
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treating the slides with alkaline alcohol too briefly
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