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41 Cards in this Set

  • Front
  • Back
Glycogen is best demonstrated by the use of:
PAS with and without diastase
Amyloid can be demonstrated with:
Congo red
The Schiff reaction demonstrates:
aldehydes
A good control for the Mayer mucicarmine stain is:
appendix
A good control for glycogen is:
liver
To increase the specificity for amyloid, Congo red stains should be examined by which microscope?
polarizing
Of the following, which is the best fixative for glycogen?

a) Zenker
b) Orth
c) acetic acid
d) absolute alcohol
d) absolute alcohol
Periodic acid is used in the PAS technique as a/an:
oxidizer
The crystal violet stain for amyloid is a/an:
polychromatic stain
Acid mucopolysaccharides are demonstrated by:
alcian blue
The reliability of the Schiff reagent may be checked by adding which of the following to a small aliquot of the schiff solution?

a) sodium iodate
b) formaldehyde
c) potassium metabisulfite
d) diastase
b) formaldehyde
A fluorescent dye used for the demonstration of amyloid is:
thioflavin T
The alcian blue stain performed at pH 1.0 demonstrates:
sulfated acid mucopolysaccharidess
Substances stained positively with the colloidal iron will be:
blue
Schiff reagent is a/an:
reduced solution of basic fuchsin
Diastase digestion increases specificity for:
glycogen
Colloidal iron is used for the demonstration of:
acid mucopolysaccharides
A mucicarmine stain would be most helpful in the diagnosis of:
adenocarcinoma
Adjacent sections are stained with PAS, one with and one without diastases digestion. A positive result on the one without digestion and a negative result on the one with digestion indicates the presence of:
glycogen
T/F: Glucose, sucrose, and other oligopolysaccharides can be demonstrated easily in tissue sections.
false
T/F: Hyaluronidase is used to digest some connective tissue mucins.
true
T/F: The routine alcian blue stain is done at pH 1.5.
false
T/F: Good Schiff reagent should be light pink.
false
T/F: The end product in the colloidal iron method is Prussian blue.
true
T/F: Amyloid shows a yellow birefringence following staining with Congo red.
false
T/F: Glutaraldehyde is one of the recommended fixatives for the PAS reaction.
false
T/F: The Schiff reaction may show false positively following chromate-containing fixatives.
false
T/F: Glycogen-containing tissue fixed in Bouin solution may show resistance to diastase digestion.
true
T/F: The addition of acid to the crystal violet staining solution reduces background staining.
true
T/F: 4um to 6um sections are recommended for crystal violet stains.
false
What reagents are used to show acid mucopolysaccharides?
-alcian blue, pH 1.0

-alcian blue, pH 2.5

-colloidal iron
What reagents are used to show amyloid?
-Congo red

-crystal violet

-thioflavin T
What reagents are used to show carboxylated acid mucopolysaccharides?
-alcian blue, pH 2.5

-colloidal iron
What reagents are used to show glycogen?
-diastase

-periodic acid

-Schiff reagent
What reagents are used to show neutral polysaccharides?
-periodic acid

-Schiff reagent
Which reagents are used to show sulfated (only) acid mucopolysaccharides?
-alcian blue, pH 1.0
No staining of the glomerular basement membrane can be seen microscopically on a control section of kidney. This may be the result of:
inadequate oxidation
Marked nonspecific background staining is noted on a section stained with the PAS technique. This could be the result of:
fixation with glutaraldehyde
Very weak staining is noted on a PAS-stained control section of liver. One problem-solving action is to:
check the Schiff reagent with formaladehyde
Sections of small intestine show orange goblet cells that are partially obscured by the yellow background. This is most likely the result of:
overstaining with metanil yellow
Control sections stained with Congo red show only yellow, and no green, birefringence. This could probably be corrected in the future by:
ensuring that the sections are cut at 8um to 10um