Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
63 Cards in this Set
- Front
- Back
|
What is the fixative for PAS Stain?
1. Zenker 2. Bouin 3. 10% NBF 4. Helly |
3. 10% NBF
|
|
|
What thickness should tissue be cut for the PAS Stain?
|
4 - 5 um/ kidney 1 - 2 um
|
|
|
What is the control tissue for the PAS Stain?
|
kidney/liver
|
|
|
What are the major reagents for the PAS Stain?
|
Periodic Acid
Schiff Reagent Harris Hematoxylin Hydrochloric Acid Potassium Metabisulfate Tap Water |
|
|
What is the reaction of the PAS stain based on?
|
The oxidation of certain elements to aldehydes by periodic acid.
|
|
|
What does the PAS stain demonstrate?
|
Simple polysaccharides
neutral mucosubstances some acid mucosubstances fungus glycogen |
|
|
What are the expected results of the PAS Stain?
|
A positive results will stain bright rose.
|
|
|
Which fixative will cause marked nonspecific background staining in the PAS Stain?
|
Glutaraldehyde
|
|
|
What does Schiff Reagent demonstrate?
|
Aldehydes
|
|
|
What can be added to schiff reagent to test its reliability?
|
Formaldehyde
|
|
|
What is schiff reagent?
|
A reduced solution of basic fuchsin.
|
|
|
What is the preferred fixative for PAS with Diastase?
|
10% NBF or absolute alcohol
|
|
|
What is the thickness of tissue for PAS with Diastase?
|
4 - 5 um
|
|
|
What control tissue is used for the PAS with Diastase stain?
|
Liver
|
|
|
PAS with Diastase will may show resistance to tissue fixed in what fixative?
|
Bouin
|
|
|
What are the major reagents in PAS with Diastase?
|
Periodic Acid
Hydrochloric Acid Schiff Reagent Potassium Metabisulfite Malt Diastase Solution Phosphate Buffer Water |
|
|
Which reagent acts as an oxidizer in the PAS stain?
|
Periodic Acid
|
|
|
What is the principle of the PAS with Diastase?
|
To depolymerize glycogen into smaller sugar units that are washed out of the tissue on the "without digestion" slide.
|
|
|
What is the purpose of the PAS with Diastase stain?
|
Demonstration glycogen
|
|
|
What are the expected results from the PAS with Diastase stain?
|
Glycogen - bright rose red on slide labeled without. Will be absent on slide labeled with.
|
|
|
What is the fixative used with the Mayer Mucicarmine stain?
|
10% NBF
|
|
|
What is the preferred thickness of tissue used with the Mayer Mucicarmine stain?
|
4 - 5 um
|
|
|
What is the control tissue used with the Mayer Mucicarmine stain?
|
Colon, small bowel, or appendix.
|
|
|
What are the major reagents used in the Mayer Mucicarmine stain?
|
Mucicarmine Stock, metanil yellow, Weigert Iron Hematoxylin.
|
|
|
What is the principle of the Mayer Mucicarmine stain?
|
It stains carboxylated and sulfated mucins but not neutral mucins.
|
|
|
What is the purpose of the Mayer Mucicarmine stain?
|
Staining epithelial mucin.
|
|
|
What are the expected results of the Mayer Mucicarmine stain?
|
Mucin - deep rose to red
Cryptococcus - deep rose to red nuclei - black other tissue - blue or yellow |
|
|
What is the fixative for Alcian Blue, pH 2.5?
|
10% NBF or Bouin
|
|
|
What is the thickness of the tissue for Alcian Blue 2.5?
|
4 - 5 um
|
|
|
What is the control tissue for Alcian Blue 2.5?
|
Small intestine, appendix, or colon.
|
|
|
What are the major reagents used in Alcian Blue 2.5?
|
Acetic Acid
Alcian Blue Solution Nuclear Fast Red |
|
|
When used in a 3% acetic acid solution, what does alcian blue stain?
|
Sulfated and carboxylated acid mucopolysaccharides.
|
|
|
What is the purpose of the Alcian Blue 2.5 stain?
|
To demonstrate acid mucopolysaccharides.
|
|
|
What are the results of the Alcian Blue 2.5 stain?
|
Weakly acidic sulfate mucosubstances - dark blue
hyaluronic acid - dark blue background - pink to red nuclei - red |
|
|
What is the fixative used with the Alcian Blue 1.0?
|
10% NBF or Bouin
|
|
|
What is the tissue thickness with the Alcian Blue 1.?
|
4 - 5 um
|
|
|
What is the control tissue used with the Alcian Blue 1?
|
small intestine, appendix, or colon
|
|
|
What are the major reagents for the Alcian Blue 1.0?
|
Hydrochloric Acid
Alcian Blue Solution Nuclear Fast Red Solution |
|
|
What is the principle of the Alcian Blue 1.0?
|
Alcian Blue stains only sulfated acid mucopolysaccharides.
|
|
|
What is the purpose of the Alcian Blue 1.0 stain?
|
Demonstrates sulfated mucosubstances.
|
|
|
What are the expected results of the Alcian Blue 1.0?
|
Sulfated mucosubstances - pale blue
background - pink to red nuclei - red |
|
|
What is the fixative used for the Colloidal Iron stain?
|
10% NBF/carnoy solution or alcoholic formalin
|
|
|
What is the tissue thickness when doing the Colloidal Iron stain?
|
4 - 5 um
|
|
|
What is the control tissue used with the Colloidal Iron stain?
|
small bowel, appendix, or colon
|
|
|
What are the major reagents in the Colloidal Iron Stain?
|
Ferric Chloride
Nuclear Fast Red Potassium Ferrocyanide Hydrochloric Acid Acetic Acid |
|
|
What is the principle of the Colloidal Iron stain?
|
Colloidal ferric ions are absorbed by carboxylated and sulfated mucosubstances.
|
|
|
What is the purpose of the Colloidal Iron Stain?
|
Demonstate carboxylated and sulfated mucopolysaccharides and glycoproteins. Prussian blue reaction is used to demonstrate iron bound to tissue.
|
|
|
What are the expected results of the Colloidal Iron stain?
|
Acid mucopolysaccharides - deep blue
nuclei - pink to red cytoplasm - pink |
|
|
What is the fixative used with Alcian Blue with Hyaluronidase?
|
10% NBF
|
|
|
What is the tissue thickness when using Alcian Blue with Hyaluronidase?
|
4 - 5 um
|
|
|
What is the control tissue when using Alcian Blue with Hyaluronidase?
|
2 sections of umbilical cord - one with and one without
|
|
|
What are the major reagents in Alcian Blue with Hyaluronidase?
|
Potassium Phosphate, Monobasic
Sociam Phosphate, Dibasic Buffer Solution Hydraluronidase Digestion Solution Alcian Blue uclear Fast Red |
|
|
What is the principle of the Alcian Blue w/ Hyaluronidase?
|
Staining will disappear when tissue contains hyaluronic acid, chondroitin sulfate A, or chondroitin sulfate C
|
|
|
Purpose of the Alcian Blue w/ Hyaluronidase stain?
|
Differentiation of epithelial and connective tissue mucins.
|
|
|
What are the results of the Alcian Blue w/ Hyaluronidase stain?
|
Without digestion, acid mucopolysaccharides - deep blue
with digestion, mucosubstances containing hyaluronic acid, chondroitin sulfate A, or chondroitin sulfate C - marked loss of staining, |
|
|
What is the fixative used with the Congo Red stain?
|
Alcohol or Carnoys/10% NBF, Bouin, Zenker
|
|
|
What is the thickness for tissue using the Congo Red stain?
|
8 - 10 um using a polarizing microscope
|
|
|
What is the control tissue when using the Congo Red stain?
|
tissue containing amyloid
|
|
|
What are the major reagents in Congo Red stain?
|
Alcohol saturated w/ sodium chloride/Alkaline Salt
Congo red staining solution |
|
|
What is the technique of the Congo Red Stain?
|
Green birenfringence is considered the most specific technique for demonstrating amyloid.
|
|
|
What is the purpose of the Congo Red stain?
|
Demonstration of amyloid
|
|
|
What are the expected results when using the Congo Red stain?
|
Amyloid - deep pink to red
Elastic tissue - pale pink Nuclei - Blue |
|
|
What is one of the most critical steps in the Congo Red stain?
|
Making sure the thickness is accurate. Will cause a yellowing.
|
