Bsc 1010L Ch 17

Front 1

1. The most recent techniques developed in the biological sciences allow the manipulation of DNA with the ultimate goal of intervening directly with the _______ fate of organisms.
A. genetic
B. developmental
C. adult
D. mitotic

Back 1

A. genetic

Front 2

2. Small circular, extrachromosomal DNA segments are known as _______.
A. vectors
B. plasmids
C. clones
D. RNA

Back 2

B. plasmids

Front 3

3. Enzymes that cleave DNA at specific sites are called _____________.
A. vectors
B. peptidases
C. restriction endonucleases
D. DNAses

Back 3

C. restriction endonucleases

Front 4

4. Single-stranded complementary tails that are produced by restriction digestion are called ______ ends.
A. sticky
B. orphaned
C. vectors
D. 5'

Back 4

A. sticky

Front 5

5. Bacterial plasmids and artificial chromosomes are used as ______ to insert foreign DNA into host cells and create recombinant genomes.
A. clones
B. vectors
C. endonucleases
D. subunits

Back 5

B. vectors

Front 6

6. Gel ________ is a process that separates DNA or protein fragments according to their size, by causing them to migrate within a gel in response to an electric field.
A. duplication
B. blotting
C. electrophoresis
D. migration

Back 6

C. electrophoresis

Front 7

7. The _________ from Agrobacterium tumefaciens is used in genetic engineering involving crop plant genes.
A. restriction endonucleases
B. DNA
C. Ti vector
D. Ti plasmid

Back 7

D. Ti plasmid

Front 8

8. A ______ vaccine is produced by using only the non-infectious part of the viral genome.
A. immunization
B. subunit
C. bacterial
D. prokaryotic

Back 8

B. subunit

Front 9

9. You want to insert your favorite gene, which has been digested with Ncol endonuclease on both ends, into a vector. Ncol recognizes the DNA sequence C*CATGG. The vector you want to use contains many endonuclease recognition sites, but does not include an Ncol site. Which restriction endonuclease would be the most suitable replacement to digest the vector?
A. Sphl - G*CATGC
B. Sunl - C*GTACG
C. Kpnl - GGTAC*C
D. CViAll - C*ATG

Back 9

A. Sphl - G*CATGC

Front 10

10. Single-stranded ends generated by the same restriction enzyme are complementary to each other. They can be joined together,
A. even when the source of the DNA is different.
B. only when the source of the DNA is the same.
C. but the "sticky ends" will most likely have to be modified.
D. but the hybridization of the two ends may cause a problem with cloning.
E. only if the subunits have been methylated.

Back 10

A. even when the source of the DNA is different.

Front 11

11. Two strands of DNA that have been cut by an endonuclease can be sealed together by a(n)
A. polymerase enzyme.
B. ligase enzyme.
C. exonuclease enzyme.
D. protease enzyme.
E. methylase enzyme.

Back 11

B. ligase enzyme.

Front 12

12. In Southern blotting, the probe is made of RNA or single-stranded DNA. What kind of probe should be used in a Northern blot?
A. only RNA
B. only single-stranded DNA
C. double-stranded DNA
D. RNA or single-stranded DNA

Back 12

D. RNA or single-stranded DNA

Front 13

13. All of the following involve molecular biology techniques except
A. ligating DNA fragments together.
B. using restriction enzymes to cut specific sequences of DNA.
C. using a DNA polymerase to copy DNA via PCR.
D. creation of Okazaki fragments.

Back 13

D. creation of Okazaki fragments.

Front 14

15. Restriction enzymes are
A. proteases.
B. lipases.
C. endonucleases.
D. exonucleases.

Back 14

C. endonucleases.

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16. An enzyme that has been isolated from retroviruses and is used to generate cDNA is
A. transverse ligase.
B. reverse transcriptase.
C. reverse endonuclease.
D. recombinant RNase.
E. RNA polymerase.

Back 15

B. reverse transcriptase.

Front 16

17. Identify a sequence that is most likely to be recognized by a restriction enzyme.
A. GATC
B. CCCTTT
C. TGGCCA
D. TCATCA

Back 16

C. TGGCCA

Front 17

18. The most common way to follow bacterial transformation with a plasmid is by
A. manufacturing the bacterial protein.
B. conferring antibiotic resistance.
C. separating the altered cell surface.
D. making the cells immortal.
E. altering the physical appearance of the cells.

Back 17

B. conferring antibiotic resistance.

Front 18

19. You have discovered a very small amount of DNA from an ancient organism that you want to save and study. Assuming the genetic code is the same as in modern DNA, what is the best way to manipulate the DNA so you can study it in the lab?
A. Southern blotting
B. Gel electrophoresis
C. PCR amplify the DNA
D. Insert the DNA into a vector

Back 18

C. PCR amplify the DNA

Front 19

20. A researcher wants to use a molecular technique to find the DNA sequence ATGGGCCAGCT in the genome of mice. He should use the __________ technique, with __________ as a probe.
A. Western blotting; TACCCGGTCGA
B. Southern blotting; ATGGGCCAGCT
C. Southern blotting; TACCCGGTCGA
D. gel electrophoresis; DNA dye
E. gel electrophoresis; ATGGGCCAGCT

Back 19

C. Southern blotting; TACCCGGTCGA

Front 20

22. If the lacZ gene is functional it produces an enzyme known as ____________, which allows the bacterial cell to metabolize the sugar, X-gal.
A. beta-glucosidase
B. beta-xylosidase
C. gamma-endonuclease
D. alpha-galactosidase
E. beta-galactosidase

Back 20

E. beta-galactosidase

Front 21

23. A commercially significant human protein now produced in bacteria is
A. hemoglobin.
B. gamma globulins.
C. AZT.
D. human insulin.
E. HIV vaccine.

Back 21

D. human insulin.

Front 22

24. Which step of PCR is most likely to require a heat stable polymerase?
A. Synthesis
B. Annealing of primers
C. Denaturation
D. PCR does not require a heat stable polymerase.

Back 22

C. Denaturation

Front 23

25. Crop plants have been protected from certain insects by the addition of a gene for a specific insect toxin from the bacterium
A. Agrobacterium tumefaciens.
B. Bacillus thuringiensis.
C. Herpes simplex.
D. Pseudomonas.
E. tobacco mosaic.

Back 23

B. Bacillus thuringiensis.

Front 24

26. Scientists can distinguish between DNA of different individuals, thus making this information useful in criminal investigations. The technique used is called
A. restriction fragment length polymorphisms.
B. societal polymorphisms.
C. hybridization polymorphisms.
D. Southern Blot.
E. molecular hybridization.

Back 24

A. restriction fragment length polymorphisms.

Front 25

27. A subunit vaccine is being developed for a newly discovered virus. Mammalian cells would be used to create a subunit vaccine composed of
A. recombinant vaccinia virus with mRNA from the newly discovered virus.
B. recombinant vaccinia virus with the outside coat of the newly discovered virus.
C. recombinant vaccinia virus with the outside coat of the hepatitis virus.
D. the newly discovered virus with the outside coat of the vaccinia virus.

Back 25

B. recombinant vaccinia virus with the outside coat of the newly discovered virus.

Front 26

28. Some of the useful applications of genetic engineering include all of the following except
A. bacteria that can digest oil in an oil spill.
B. growing glyphosate-resistant cotton.
C. development of hybrid corn to increase yield.
D. creation of Golden Rice.
E. salmon expressing a transgenic growth hormone gene.

Back 26

C. development of hybrid corn to increase yield.

Front 27

29. A key difference between transforming plant cells and human cells is
A. genes to be expressed in human cells require a eukaryotic promoter, and genes to be expressed in plants don't.
B. DNA cannot be introduced into human cells.
C. Agrobacterium is commonly used as a tool to directly transform plant cells but not human cells.
D. a vector is needed to introduce DNA into human cells and not plant cells.

Back 27

C. Agrobacterium is commonly used as a tool to directly transform plant cells but not human cells.

Front 28

30. Imagine a small section of the human population evolved the requirement to make their own aromatic amino acids. How would eating glyphosate-resistant plants affect these individuals?
A. No effect.
B. They would become very sick from the glyphosate that was sprayed on the plants.
C. They would also become glyphosate-resistant.
D. They would no longer need to make their own aromatic amino acids.

Back 28

B. They would become very sick from the glyphosate that was sprayed on the plants.

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31. An insecticidal protein has been discovered in a bacterium known as
A. Escherichia coli.
B. Agrobacterium tumefaciens.
C. Bacillus thuringiensis.
D. Aspergillus sp.

Back 29

C. Bacillus thuringiensis.

Front 30

33. A fourth type of restriction enzyme is identified that cleaves at precise locations like a type II enzyme, but it also occasionally makes mistakes and cuts elsewhere. Would this type of enzyme be a useful enzyme to use in molecular cloning?
A. Yes, because it cuts correctly sometimes.
B. No, because it is a precise enzyme.
C. No, because cutting mistakes could lead to incorrect cloning.
D. Yes, because cutting mistakes don't matter in molecular cloning.

Back 30

C. No, because cutting mistakes could lead to incorrect cloning.

Front 31

34. A new vector contains a gene for GFP, green fluorescent protein in the multiple-cloning site. When the vector is transformed into bacteria, the bacteria glow green, but they won't if another gene is inserted. This is an example of
A. an origin of replication.
B. reverse genetics.
C. gene therapy.
D. insertional inactivation.

Back 31

D. insertional inactivation.

Front 32

35. Technique used to amplify a desired piece of DNA.
A. gel electrophoresis
B. RFLP
C. PCR
D. plasmids
E. Northern Blot

Back 32

C. PCR

Front 33

36. A nucleotide change from T to C in a green pigment protein is responsible for certain cases of colorblindness. To test if someone who is colorblind has this mutation, you should use
A. gel electrophoresis.
B. molecular cloning.
C. RNAi.
D. RFLP analysis.

Back 33

D. RFLP analysis.

Front 34

37. Organisms containing DNA from an outside source are called
A. transcribed organisms.
B. compound organisms.
C. hybrid organisms.
D. plasmid organisms.
E. transgenic organisms.

Back 34

E. transgenic organisms.

Front 35

39. Bam HI is a Type II restriction enzyme that recognizes the sequence 5' GGATCC 3', and cuts between the two Gs, leaving a 5' overhang. If you were to digest DNA with Bam HI, what would be the sequence of the overhanging sticky end of the complementary strand?
A. 5' GATCC 3'
B. 5' GATC 3'
C. 5' CTAG 3'
D. 5' G 3'

Back 35

B. 5' GATC 3'

Front 36

40.

A 6.85 kb EcoRI fragment of DNA is shown below. The location of several restriction sites is indicated. Scale is approximate.

If you were to digest this fragment of DNA with Pst I, how many pieces of DNA would you obtain?

A. 1
B. 2
C. 3
D. 4

Back 36

C. 3

Front 37

41.

A 6.85 kb EcoRI fragment of DNA is shown below. The location of several restriction sites is indicated. Scale is approximate.

If you were to digest this DNA with PstI, which length of DNA fragment would migrate the fastest on an agarose gel?

A. 803
B. 2622
C. 3425
D. 235

Back 37

A. 803

Front 38

42. You are trying to clone a piece of DNA into a plasmid vector that exhibits blue-white selection. Following ligation and transformation, you plate the host cells onto several plates containing the appropriate selective media. The next day you examine the bacterial colonies that grew. Which of the following plates should you select to proceed with the analysis of possible transformants?
A. 3 white colonies, 50 blue colonies
B. 97 white colonies, 105 blue colonies
C. 43 white colonies, 6 blue colonies
D. 238 blue colonies

Back 38

C. 43 white colonies, 6 blue colonies

Front 39

43. A researcher identified a new enzyme and is interested in determining the mRNA expression pattern of this gene. Which of the following techniques would accomplish this goal?
A. Southern blot
B. Northern blot
C. Western blot
D. Eastern blot

Back 39

B. Northern blot

Front 40

44. You are hired as a scientific consultant for a popular television show about forensic analysis of crime scene evidence. You are told that in an upcoming scene, investigators will compare the DNA profiles of a known suspect and an unknown biological sample collected from the victim. You are asked to write up a brief explanation of the primary technique required for discussion in the scene. Which technique should you write about?
A. DNA fingerprinting
B. DNA footprinting
C. Northern blot
D. RNAi

Back 40

A. DNA fingerprinting

Front 41

45. Which of the following types of information would be most useful in an effort to move a DNA fragment from one plasmid vector to another, using molecular cloning techniques?
A. restriction fragment length polymorphism data
B. sequence of PCR primers
C. Southern blot data
D. restriction maps of the plasmids

Back 41

D. restriction maps of the plasmids

Front 42

46. Which of the following two enzymes are needed to construct a cDNA library?
A. reverse transcriptase and DNA ligase
B. reverse transcriptase and Taq polymerase
C. DNA ligase and Taq polymerase
D. Taq polymerase and RNA polymerase

Back 42

A. reverse transcriptase and DNA ligase

Front 43

47. The development of PCR has revolutionized molecular biology and has led to numerous advances in basic research, as well as in medicine and forensic science. Which of the following areas does not rely on PCR?
A. Diagnosis of genetic diseases
B. Analysis of mitochondrial DNA from early human species
C. Generation of hybrid plants
D. Identifying individuals involved in crimes

Back 43

C. Generation of hybrid plants

Front 44

50. In order to make a successful subunit vaccine you need
A. protein purified from the pathogen of interest.
B. the complete DNA sequence of the pathogen.
C. DNA sequence for a protein expressed on the surface of the pathogen.
D. active pathogen.

Back 44

C. DNA sequence for a protein expressed on the surface of the pathogen.

Front 45

51. A major new breakthrough for gene therapy would be
A. the ability to deliver RNAi gene therapy.
B. the ability to add a working copy of a missing gene.
C. the ability to insert genes into a specific location in the human genome.
D. the ability to insert genes randomly in the human genome.

Back 45

C. the ability to insert genes into a specific location in the human genome.

Front 46

53. Methane produced by cows is a major environmental issue. Scientists are working to genetically modify cows to produce less gas. This concept is most similar to
A. the Enviropig.
B. large transgenic salmon.
C. dog breeding.
D. Golden Rice.

Back 46

A. the Enviropig.

Front 47

54. Some food crops are difficult to grow for a variety of reasons. For example, strawberries are easily susceptible to frost damage. How could one use genetic engineering techniques to help overcome this type of problem, and which type of animal or plant would one look to for help?
A. To increase the frost hardiness of strawberries, one could generate a strain of transgenic strawberries using a gene that confers frost resistance. To identify such a gene, one could look to an animal or plant that lives in a very cold environment. Such organisms are likely to have some form of natural protection against frost.
B. To increase the frost hardiness of strawberries, one could generate a strain of cloned strawberries using a gene from another plant that confers frost resistance.
C. To increase the frost hardiness of strawberries, one could generate a strain of strawberries using a breeding design that confers frost resistance. By beginning with northern strawberry strains, breeding and selecting those that survive colder temperatures would eventually produce frost hardiness.

Back 47

A. To increase the frost hardiness of strawberries, one could generate a strain of transgenic strawberries using a gene that confers frost resistance. To identify such a gene, one could look to an animal or plant that lives in a very cold environment. Such organisms are likely to have some form of natural protection against frost.

Front 48

56. Investigators have found a new bacteria that lives under both aerobic and anaerobic conditions. They hypothesize that different genes are expressed under the two conditions. What experiments need to be performed in order to confirm this hypothesis and identify the responsible genes?
A. Completely sequence the genome of the bacteria in both aerobic and anaerobic conditions
B. Collect expressed RNA from both anaerobic and aerobic conditions and generate two cDNA libraries
C. Compare the bacterial growth characteristics to other bacteria that have similar growth characteristics
D. Design primers to a specific region of the genome and sequence in both anaerobic and aerobic conditions

Back 48

B. Collect expressed RNA from both anaerobic and aerobic conditions and generate two cDNA libraries