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28 Cards in this Set
- Front
- Back
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What type of fixative is in a...
...purple top tube? What does this material do? |
EDTA;
chelates Ca++ preventing clotting |
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What type of fixative is in a...
...red top tube? What is it used for? |
nothing! just vacuum! It sucks!
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What type of fixative is in a...
...tiger top tube? What does it do? |
serum separator tube (waxy stuff to help separate serum from RBCs)
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What type of fixative is in a...
...green top tube? What does this do? |
Heparin;
binds with antithrombin III preventing coagulation |
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What type of fixative is in a...
...teal (light blue) top tube? What is this used for? |
sodium citrate;
used for clotting tests (PT, PTT, vonWillebrand's) |
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What type of fixative is in a...
...gray top tube? What does this do? |
Fluoride oxalate;
inhibits glycolysis |
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T or F:
A purple top tube can be used for blood chemistries. |
Flase! Yeah I said flase! So what of it?
Anyhow, the EDTA in purple tops is potassium salt and thus F's up the K+ and the Ca++ values |
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Which tube is the best tube to use for blood count?
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Purple top (EDTA)
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Which tube is the best for blood chemistries?
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Green top (heparin)
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Which tube can affect hormone assays?
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Tiger top
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T or F:
Green top tubes can be used for plasma collection. |
True!
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Which tube is the most sensitive to the volume of blood collected?
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Light Blue (Teal) top - has a lot of sodium citrate in it
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What should you do before you run any test?
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MIX THE BLOOD, DUMBASS!!!
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When performing a Diff Quick stain, what are in the 3 wells (in order)?
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1. methanol (fixative)
2. xanthine (orange dye) 3. thiazine (purple/blue dye) |
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What lab technique is used to measure plasma protein?
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Refractometry
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Where should the PCV be read, at the top or bottom of the buffy coat?
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bottom
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What cellular structures take up stain in a new methylene blue stain?
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reticulum (ribosomes and ER)
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Reticulocytes are counted as (choose one)...
a) % of 100 cells b) % of 1000 cells c) % of 10000 cells d) cells per fL |
b) % of 1000 cells
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What is weird about cat reticulocytes?
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There are normal (aggregate) retics and punctate retics
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What cells are found in the buffy coat?
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Leukocytes
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Which protein contributes the most to blood density?
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Albumin
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What steps are necessary to determine fibrinogen?
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1) total plasma protein
2) precipitate fibrinogen in water bath (in 2nd PCV tube) 3) take 2nd plasma protein reading 4) difference in plasma protein = fibrinogen |
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What units are fibrinogen measured in?
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mg/dL
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In a nucleated blood cell count, what must be done before staining and fixing the WBCs?
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Lyse the RBCs
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What tool is used for counting nucleated cells?
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hemocytometer
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T or F:
A hemocytometer is used for performing a differential WBC count. |
False!
The differential WBC count is done on a standard smear (or schmear). |
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Which pattern is the best to use when examining a smear when performing a differential WBC count?
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Um...I see a unicorn holding a baby...wait...this isn't a Rorschasch test! The answer is.....
C |
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When should a WBC count be corrected?
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When there are 5 or more nucleated RBCs found when performing a differential WBC count.
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