Bordatella

Front 1

bordetella

Back 1

small gram neg cocci
aerobic,
REQUIRE SPECIAL MEDIA:

Front 2

Is there bordetella pneumonia?

Back 2

no

Front 3

other pertussis organisms?

Back 3

parapertussis (mild diseaes)
bronchiseptica (cats and dogs)

Front 4

virulence factors?

Back 4

fimbrae hemagluttinin
like fimbrae but shorter and essential for colonization!
Also, fha is major component of vaccine

Front 5

toxins....

Back 5

Pertussis toxin (another major vaccine component)
tracheal cytotoxin
adenylate cyclase toxin

Front 6

Pertussis toxin

Back 6

b subunit binds to phagocytic cells and triggers endocytosis which gets A subunit into cytoplasm. S1 inhib monocyte and neut activation. incs cyc amp in cell. S1 is an adp ribose transferase.
Also causes lymphocytosis

Front 7

tracheal toxin

Back 7

stops ciliated cells from beating

Front 8

adenylate cyclase toxin

Back 8

adds bacterial adenylate cyclase into host cell to further increases camp

Front 9

where does it live?
human carriers?
transmission?

Back 9

nasopharynx
Not much at all to speak of
person to person, inhalation and shared secretions

Front 10

other pertussis effects

Back 10

seizures, secondary infections of ears and sinuses lungs

Front 11

best detection technique

Back 11

pcr

Front 12

culture is bordet gengou blood agar or regan-lowe culture

Back 12

regan low is agar with blood and charcoal and abs

Front 13

treatment

Back 13

erythromycin

Front 14

environmental bacteria: legionella , pseudomonas, acinetobacter

Back 14

yes

Front 15

pseudomonas

Back 15

gram neg rods, obligate aerobes, motile (can't tell from ecoli and klebsiella, etc)

Front 16

where found

Back 16

soil and water

Front 17

pseudomonas major disease causing in what pop?

Back 17

immunocomp and
cystic fibrosis

Front 18

which species major pathogens?

Back 18

p aeruginosa, stenotrophomonas maltophilia, burkholderia cepacia account for 80%

Front 19

I say CF you say?

Back 19

p aeruginosa pneumonia

Front 20

bio terrorism possibilities?

Back 20

burkholderia mallei and pseudomallei; acute severe pneumonia

Front 21

aeromonas hydrophila

Back 21

common cause of wound infections, especially in fresh water

Front 22

purple colonies?

Back 22

chromobacterium violaceum

Front 23

elizabethking meningosepticum:

Back 23

can cause meningitis

Front 24

acinetobacter baumannii

Back 24

first emerged as wound/bloodsttream infection in soldiers in iraq, trnsmitted from soil and water.
Nosocomial pneumonia

Front 25

p aeruginosa

Back 25

very resistant to many disinfectants and antibiotics

Front 26

virulence factor
major one?

Back 26

capsule: antiphagocytic
slime layer: polysacc, easly lost
Can change and make a mucoid capsule: alginate polysacchardide exclusive for cf strains

Front 27

others?

Back 27

a bunch of toxins
(he mentioned 4 specificall but we don't have to know the names)

Front 28

elastase

Back 28

degrades elastin and degrades alpha one anti tryp which makes neutrophils worse

Front 29

pigments?

Back 29

blue and yellow (possibly green)

Front 30

pseudomonas in cystic fibrosis

Back 30

doesn't go to blood stream

Front 31

burns?

Back 31

pseudomonas again

Front 32

pseudomonas color of pus?

Back 32

green

Front 33

uti

Back 33

pseudomonas associated with catheter

Front 34

whirlpools can give you?

Back 34

pseudomonas dermatitis (folliculitis)

Front 35

pseudomonas can also give you?

Back 35

swimmer's ear (acute otitis externa)

Front 36

also get?

Back 36

malignant otitis externa (associated with diabet)

Front 37

corneal infections?

Back 37

yes, especially through mascara brush damages

Front 38

endocarditis?

Back 38

IV drug users

Front 39

diagnosis?

Back 39

the general stuff

Front 40

treatment?

Back 40

piperacillin/tazobactam, 3 gen cephs, carbapenems, fluoroquin, aminoglycosides

Front 41

legionella

Back 41

yes

Front 42

disease?

Back 42

atypical pneumonia

Front 43

where does it live?

Back 43

inside aquatic protozoa

Front 44

which most common disease?

Back 44

l pneumophila

lp serogroup 1 only legionella in community acquired infections

Front 45

distinctive props

Back 45

gram neg rods
fastidious (need chocolate agar)
slow growth and strict aerobe
Intracellular growth inside lungs happens in macrophages!!!! (he said we should remember this)

Front 46

pontiac fever:

Back 46

legionella mild respiratory disease

Front 47

diagnosis

Back 47

atypical; have to leave legionella specific tests;
urine antigen test very important now.

Front 48

trtmnt

Back 48

azithromycin, now better survival with fluoroquins....can combine