• Shuffle
Toggle On
Toggle Off
• Alphabetize
Toggle On
Toggle Off
• Front First
Toggle On
Toggle Off
• Both Sides
Toggle On
Toggle Off
• Read
Toggle On
Toggle Off
Reading...
Front

### How to study your flashcards.

Right/Left arrow keys: Navigate between flashcards.right arrow keyleft arrow key

Up/Down arrow keys: Flip the card between the front and back.down keyup key

H key: Show hint (3rd side).h key

A key: Read text to speech.a key

Play button

Play button

Progress

1/29

Click to flip

### 29 Cards in this Set

• Front
• Back
 What happens to light every time it hits a surface? 4% of it is lost because of reflection and refraction. snell's law n1sin(a)=n2sin(b) index of refraction n = ? speed of light in vacuum/ speed of light in medium c/v1 what is the critical angle? critical angle is the angle needed to determine if total internal reflection will occur. it is: sin(critical angle) = nB/Na where Nb and Na are indexes of refraction total internal reflection occurs for angles higher than critical angle. Numerical aparture Na= n sin(a) (light gathering capacity) sin(a) is half angle of cone of light n is index of refraction of medium How to determine if a virtual or real image is formed? a real image is formed when n1n2 in a ) shaped lens (N2-N1)/r = (n1/p) + (n2/q) p- distance btw object and interface q distance between image and interface (if negative it is a virtual image) r- radius of curvature of lens What is the thin lens equation and what does it tell you? (1/f) = 1/p + 1/q Tells you about the position of the object and the magnification of the image f is the focal length p is distance from object to center of lens q is the distance from the lens to the image. spot size and area f(wavelength)/d d is diameter of raw bean f is focal length area = (∏(ss)^2)/4 What are the two ways to characterize light in terms of wavelength? spacialy: (x position is on the x axis) k vector = 2pi/wavelength wave number: k = 1/wavelength tmporally (time is on the x axis) angular frequency = 2pi/period frequency= 1/period what is frequency in terms of wavelenths and speed of light? v= c/wavelength what is c 2.998 x 10^8 m/s energy of a photon? E= hv E= hc/wavelength h= 6.626 x 10^-34 Js what is the range of visible light? 350 - 720 nm violet red what are the properties of lasers? columnated - rats are parallel coherent- all rays are in phase monochromatic what is the intensity of a laser? (I) power/area same as irradience power can be average or peak what is raleigh scattering? re-emitted wavelength = incident wavelength what is raman scattering? re-emited wavelength = incident wavelength peak power of a laser? Pk = Energy/ period fluence (focused energy of a laser) E/area energy per pulse of pulse laser? P avg/ rep. rate Lambert-Beer law? what is it and what does it decribe change of intenstiy of light as it goes through a medium: I(Z) = Iinitial e^-(Beta)z z is thickness of medium or distance traveled through medium Beta = μa + μs μa= absorbtion coefficient μs = scattering coefficiient types of microscopes? Some types of microscopes Brightfield/Transillumination (absorption) Phase contrast (scattering/phase retardation) Differential Interference Contrast scattering/refractive index gradient) Fluorescence Confocal (depth resolution; reflectance/fluorescence) Multi-photon fluorescence (depth resolution) Second Harmonic Generation (depth resolution; scattering) what is resolution the min distance between two objects in which the two objects can be distinguished as sepatare r= .61λ/Na percent contrast? % contrast = [(Ib/Is)/Ib) x 100 Ib intensity of background Is intensity of sample trans illumination illumination and imging on diff sides of specimine epi illumination illumination and imaging on same side of specimen resolution range of light microscope? .1 microms - 1mm resolution range of Electron microscope? 1.1 angstrom to 100 microns magnification M = -q/p