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15 Cards in this Set
- Front
- Back
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What does the term "respiratory control" mean?
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Refers to the availability of ADP substrate in the MM as governing the rate of oxidative phosphorylation.
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What role does creatine play in regulating OP?
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creatine can be phosphorylated and act as a reservoir for Pi molecules when ATP needs to be made suddenly.
Phosphocreatine --> Pi + Creatine is spontaneous. |
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What is an example of a poison that uncouples ET and phosphorylation?
What is the effect on the ETC rate? |
Dinitrophenol = this compound lets H+ back through the MM without going through ATP Synthase.
No gradient formed. ETC runs at maximum rate bc no back pressure. HEAT PRODUCED. |
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What poison inhibits ET at cytochrome oxidase?
How does this affect O2 consumption, ET, NADH? What is an antidote? |
Cyanide.
Decrease in O2 consumption and ET. Increase in NADH remedy = nitrate f/by thiosulfate. |
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What poison inhibits ATP/ADP translocase?
What is the main problem with this? What is the effect on H+ gradient, ET, O2 and NADH. |
Bongkrekic Acid
if this translocase is inhibited then there is no ADP in the MM to phosphorylate. H+ gradient & NADH INCREASE ET and O2 consumption DECREASE |
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What is the most sensitive measure if energy requirement?
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Level of 5'AMP....implies low ATP.
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If ATP builds up, what else builds up?
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High ATP will cause increase in NADH (bc not going through chain), Citrate (bc inhibited by NADH) and AcetylCoA (bc AcetylCoA doesn't have Pyruvate to buddy up with).
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What regulates mitochondrial enzymes in glucose metabolism?
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NADH regulates mitochondrial enzymes.
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What regulates cytoplasmic enzymes in glucose metabolism?
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ATP/ADP ratio regulates cytoplasmic enzymes.
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What is a strong allosteric inhibitor of Isocitrate Dehydrogenase?
Why? What activates isocitrate dehydrogenase? |
Increased NADH is a strong allosteric inhibitor of Isocitrate dehydrogenase.
NADH is a product of the normal rxn. If there is excess NADH...the rxn won't go. ADP ACTIVATES THIS. |
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How do increased AcetylCoA and NADH affect the PDH rxn?
What happens to Pyruvate? |
Increase ATP, AcetylCoA and NADH will cause PDH Kinase to phosphorylate PDH - this INHIBITS the rxn. No AcetylCoA is made from Pyruvate. (lactate will be made).
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What inhibits PFK?
What activates PFK? |
ATP inhibits PFK
AMP activates PFK |
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What inhibits Hexokinase?
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Glucose-6-phosphate
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What inhibits Citrate synthase rxn?
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Increased Citrate and NADH inhibits citrate synthase.
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What inhibits Pyruvate Kinase?
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ATP inhibits Pyruvate kinase
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