Blue Book Questions Mouse 3 Part 4

Front 1

Intestinal bacteria synthesize what vitamins ?

Back 1

Water soluble B vitamins and fat soluble vitamin K

Front 2

How do rodents benefit from bacteria made vitamins: i.e. bacteria make vitamins in large intestine but vitamins only absorbed in small intestine?

Back 2

Coprophagy

Front 3

Cecal volvulus is a common finding in many germ free animals. True or false.

Back 3

True

Front 4

What type of stool do germ free mice have? Consistency?

Back 4

Semisolid. Considered a chronic diarrhea.

Front 5

Small intestine of germ free animals is very thick or thin? Why?

Back 5

Thin. Lamina propria decreases because few plasma cells, decreases mucosal surfaces, etc.

Front 6

Is peristalsis in gastrointestinal tract increased or decreased in germ free as compared to conventional?

Back 6

peristalsis is decreased resulting in longer transit

Front 7

Lymphocytic tissue of germ free have fewer competent cells than conventional mice. Is there a sign of immunocompetence? Why or why not.

Back 7

No. immune response equal to conventional or greater. Decreased competent cells merely because of lack of previous antigenic stimulation but because of 'uncommitted' nature of germ free animals immune response often better.

Front 8

Where does the transformation of sulfasalazine occur in order to activate it?

Back 8

Gastrointestinal tract by intestinal flora

Front 9

All functional immunoglobulin molecules are 4 -polypeptide chain monomers consisting of how many light (L) chains and how many heavy (H) chains?

Back 9

2L & 2H

Front 10

What are the domains of which the immunoglobulin chains are made of?

Back 10

Domains are 3 - dimensional regions of globular structure which the chains fold into.

Front 11

Lp, Vk, Jk, Ck are exons. What are exons and introns?

Back 11

Exons are amino acid coding sequences. Introns are intervening non-coding sequences.

Front 12

The immunoglobulin genes are located on how many different chromosomal loci?

Back 12

3

Front 13

H chains are coded on which chromosome? L chains are coded on which?

Back 13

Heavy chain is from chromosome 12. Light chain is from chromosome 6.

Front 14

The Vh genes as well as any genes are made up of a certain sequence of what?

Back 14

Amino acids

Front 15

What are the dual functions of IgM?

Back 15

IgM secreted serves as antibody. In B lymphocytes, IgM serves as membrane proteins, acts as antigen receptor and stimulates lymphocyte to divide and differentiate into a protein secreting plasma cell.

Front 16

How many subclasses of IgG are there?

Back 16

4 subclasses

Front 17

What is the principal immunoglobulin in the CNS? Why?

Back 17

IgG. Because its small enough to pass blood brain barrier.

Front 18

Why is it important that antibodies attach to bacteria and viruses?

Back 18

So that phagocytes (neutrophils, macrophages, monocytes and eosinophils) attach to the bacteria and viruses via antibody on their surface and destroy them.

Front 19

Where does IgA do its thing and what cell produces IgA?

Back 19

IgA is produced by plasma cells in lamina propria of gut, respiratory tract and mammary gland. Consequently they do their business `outside' the body in the gut, lungs, and milk (to neonate)

Front 20

Which thymocytes are more mature: TL+ or TL-?

Back 20

TL_

Front 21

Tla alleles are on what chromosome number?

Back 21

17

Front 22

What component may take the place of TL in the plasma membrane of thymocytes of TL-mouse strains?

Back 22

h-2D antibody

Front 23

What are the following letter: TL, Thy-1, Lyt-1, Lyt-2, Lyt-3, Gix? What about Lyb-2, Pca-1, PC, Lyb-3, Lyb-5?

Back 23

The first group are the immunogenetic systems of the T cells. The second group is for B cells.

Front 24

B-lc gives rise to antibody secreting cells and ______ cells.

Back 24

Plasma

Front 25

A second lymphocyte population is derived from the thymus and cells of _____?

Back 25

T cells

Front 26

Which cell (T cell or B cell) lacks detectable surface immunoglobulins?

Back 26

T cells

Front 27

Describe how network of immunologic regulation works.

Back 27

Antigen consumed by macrophages ... macro communicates to regulator T cell which regulates both Helper T cells and Suppressor T cells which work together to direct B cells which secrete antibody

Front 28

SLE is characterized by an increase or decrease in serum immunoglobulins, antinuclear antibodies (ANA) and immune complex glomerulonephritis. Death therefore is usually from _____ ______.

Back 28

increase, renal insufficiency

Front 29

What mouse strain is best used for autoimmune disease?

Back 29

NZB and NZB/NZW, (BW) hybrid

Front 30

At 6 months about 50% of NZB are ___ positive; by 10 months virtually 100% are.

Back 30

Coombs

Front 31

What other mouse strain is a model for autoimmune disease in humans?

Back 31

MRL & BXSB and Palmerston North (PN)

Front 32

What is different about the BXSB strain over MRL, NZB and BW strains.

Back 32

Males rather than females develop the disease earlier and with greater severity.

Front 33

What mouse strains also are models for immune response disorders, exhibit the following disorders (clinical signs): a deficiency in the ability to develop effective immune response but increase in serum immunoglobulins (IgM), splenomegaly, lymphadenopat

Back 33

A 'motheaten' mouse. The `me' is on chromosome 6. An autosomal recessive mutation C57BL/6J

Front 34

What mouse strains also are models for immune response disorders, exhibit the following disorders (clinical signs): defective anterior pituitary, decrease in growth hormone producing acidophils, underdeveloped thyroids, adrenals and gonads, immunologic

Back 34

Snell - Bagg Dwarf mouse (dw/dw)

Front 35

What mouse strains also are models for immune response disorders, exhibit the following disorders (clinical signs): infertile, life span rarely exceeds 60 days, lack of T cells, immunodeficient disease

Back 35

Ames Dwarf mouse (df/df)

Front 36

IP injections are given in the lower right or left quadrant?

Back 36

right

Front 37

What is the usual site for IV cannulation in mice?

Back 37

Tail vein

Front 38

Anesthesia of choice for cardiac puncture? Why?

Back 38

Ether, does not effect hematocrit, RBC, WBC or differential

Front 39

How is B.P., Heart rate, and respiratory rate taken in a mouse?

Back 39

Blood pressure: Carotid cannulation, occluding cuff on tail Heart rate: ECG or estimated from blood pressure Respiratory rate: Electromyograms of diaphragm, pneumotracheograms with glass face mask or tracheal cannulas, or a light detector system

Front 40

How do you do a neuro examination in a mouse?

Back 40

Place mice in pan of warm water. Normal mice know how to swim. Placing reflexes as in dogs. Hang by tail, normal mouse abduct their hindlegs and spread their toes.