Blood Bank Component Therapy

Front 1

FDA definition of red cell storage

Back 1

allows 75% survival 24 hrs after tx

Front 2

CPD storage time

Back 2

citrate-phosph-dextrose
21 days

Front 3

CP2D storage time

Back 3

cit-phosph-dex-dex
21 days

Front 4

CPDA-1 storage time

Back 4

+ adenosine
35 days

Front 5

additive solutions

Back 5

as-1 adsol
as-2 nutricel
as-3 optisol

42 days

dex adenosine mannitol

Front 6

additive solution RBC preparation

Back 6

CPD or CP2D, spun, then +110 mL additive (500 mL) or +100 (450 mL)

Front 7

HCT of additive solution RBC

Back 7

55-65%

Front 8

RBC storage

Back 8

42 days (additives) at 1-6 deg

Front 9

frozen RBC storage

Back 9

10 years @ -65
use 24 hours after thaw

Front 10

washed RBC storage

Back 10

24 hours at 1-6 deg

Front 11

platelet storage

Back 11

5 days @ 20-24 C agitated

Front 12

pooled platelet storage

Back 12

4 hours

Front 13

granulocyte storage

Back 13

24 hours @ 20-24 deg, not agitated

Front 14

FFP storage

Back 14

1 year @ -18 C
7 year @ -65 C

Front 15

thawed FFP storage

Back 15

24 hrs @ 20-24 C

Front 16

pooled FFP storage

Back 16

4 hours

Front 17

required RBC HCT

Back 17

< 80%

Front 18

required RBC Hb

Back 18

>50 g in 95%

Front 19

LR RBC required WBC

Back 19

< 5 x 10 ^6 in 95%

Front 20

LR RBC required retained RBC

Back 20

85%

Front 21

platelet # requirement

Back 21

> 5.5 x 10 ^10

Front 22

required platelet pH

Back 22

> 6.2 in 90%

Front 23

LR platelet required number

Back 23

> 5.5 x 10^10 in 75%

Front 24

LR platelet required pH

Back 24

> 6.2 in 90%

Front 25

LR platelet required WBC num

Back 25

<8.3 x 10^5 WBC in 95%

Front 26

apheresis platelet num require

Back 26

> 3.0 x 10^11

Front 27

apheresis platelet pH require

Back 27

pH > 6.2 in 90%

Front 28

LR apheresis platelet diff require

Back 28

< 5.0 x 10^6 residual in 95%

Front 29

CRYo requireemnts

Back 29

Factor VIII > 80 IU
fibrinogen > 150 mg

Front 30

granulocyte conce requirement

Back 30

> 1.0 x 10^10 in 75%

Front 31

whole blood contents

Back 31

450-500 mL
RBC 200-250 mL
plasma 250-300 mL
WBC 10^9
platelets
anticoags 63-70 mL

Front 32

how fast can an effect of Tx be measured

Back 32

15 minutes

Front 33

RBC shipping T

Back 33

1-10 C

Front 34

compatible fluids RBC

Back 34

normosol-R, plasma-lyte
5% albumin
NS
plasma

Front 35

incompatible fluids RBC

Back 35

LR, hypo or hypertonic saline

Front 36

rejuvenated RBC

Back 36

3 days after expiration CPD, CPDA-1 or until expiration AS-1 with rejuvesol (ATP, 2,3-DPG)

Front 37

WBD platelet volume

Back 37

40-60 mL

Front 38

WBD platelet WBC num

Back 38

usually ~10^7

Front 39

neonatal platelet dose

Back 39

10-15 mL / kg

Front 40

platelet indications for thrombocytopathy

Back 40

1. congenital
2. drug
3. bypass/ecmo
4. secondary for metabolic (CRF)

Front 41

contraindications for platelets

Back 41

1. itp 2. hit 3. ttp 4. post-transfusion purpura

Front 42

expected platelet response

Back 42

20-30k rise following 1 hr

Front 43

platelet shipping temp

Back 43

20-24 C

Front 44

LR filter WBC reduction

Back 44

99.99% reduction (4 log)

Front 45

what % of original component must remain following LR

Back 45

85%

Front 46

washed red cell shelf life

Back 46

24 hours

Front 47

washed platelet shelf life

Back 47

4 hours

Front 48

NAT/NAIT

Back 48

neonatal autoimmune thrombocytopenia
maternal anti-HPA-1A
1st pregnancy
10-30% intercranial hemorrhage

Front 49

NAT/NAIT treatment prebirth

Back 49

IVIg
washed & irradiated maternal (or ag negative) platelets

Front 50

NAT/NAIT treatment postbirth

Back 50

washed & irradiated maternal or ag negative platelets

Front 51

cryopreservatives

Back 51

usually glycerol 40%
DMSO is poor, only for platelets

Front 52

which cpd can be frozen

Back 52

up to 6 day shelf life

Front 53

cpda-1 shelf life to frozen

Back 53

6 days

Front 54

cp2d shelf life to frozen

Back 54

6 days

Front 55

additive soln shelf life to frozen

Back 55

to full 42 days

Front 56

how long can rbc be frozen

Back 56

10 years @ -65 c

Front 57

how long can plt be frozen

Back 57

2 years at -80 c

Front 58

irradiation dose

Back 58

2500 cGy = rad, at least 1500 cGy to all parts.

Front 59

idx for irradiation

Back 59

1. congenital t-cell def.
2. hsct
3. anti-T cell chemo
4. aplastic anemia
5. solid tumor c ext chemorads
6. intrauterine tx
7. neonatal exchange
8. hematol malig / HL
9. granulocyte Tx
10. first degree or HLA matched

Front 60

irradiation expiry

Back 60

28 days after radiation OR normal expiry date, whatever is 1st.

Front 61

FFP volume

Back 61

200-250 mL

Front 62

FFP fibrinogen content

Back 62

400 mg

Front 63

vitamin K length to activity

Back 63

6-12 hrs

Front 64

bleeding pt with coumadin, dose

Back 64

10-20 mL/kg FFP

Front 65

expected FFP effect

Back 65

increases factor 20-30%

Front 66

FP24

Back 66

frozen within 24 hours, not 8

Front 67

FP24 factor def

Back 67

factor 8 is 25% less

Front 68

fp24 when can it be used

Back 68

anything as FFP but FFP is better for DIC

Front 69

thawed FFP good for

Back 69

24 hours

Front 70

thawed FP24 good for

Back 70

24 hours

Front 71

thawed plasma

Back 71

good for 5 days at 1-6 c
is basically thawed FFP/FP24

Front 72

liquid plasma

Back 72

plasma from blood 5 days expired
stored 1-6 c, not frozen
can be tx up to 5 days following expiry

Front 73

cryoprecipitate contents

Back 73

15 ml vol
150 mg fibrinogen
80 IU factor 8
100 IU vWF
50 IU factor 13
fibronectin

Front 74

tx uremic thrombocyopathy

Back 74

cr > 3 mg/dL
1) DDAVP 2) dialysis 3) cryo
adhesion defect

Front 75

tx vwf with cryo

Back 75

1 bag / 10 kg q 8 hrs
if severe & no factor 8 cocnentrate

Front 76

making cryo

Back 76

1 unit FFP thawed to 1-6 c
spin and remove liquid
ppt within 24 hrs
store -18 c @ 1 yr

Front 77

pooled cryo exp

Back 77

tx within 4 hrs

Front 78

PCC

Back 78

prothrombin complex concetrate
hemophilia B or warfarin overdose
(factor 9 complex concentrate)

Front 79

PPF

Back 79

plasma protein fraction
83% albumin (vs 96% in albumin)

Front 80

granulocyte concentrate contents

Back 80

200-300 mL volume
> 1.0 x 10^10 WBC
1 x 10^11 platelets
plasma and anticoagulant

Front 81

granulocyte tx background indications

Back 81

premature neonates with sepsis
transplant patients
chronic granulomatous dz

Front 82

situations for granulotcyte tx

Back 82

fever 24-48 hrs
proven infection
no response to abx
< 500/uL neutropenia
reversible bone marrow hypoplasia

Front 83

granulocytes: ta-gvhd and cmv safety

Back 83

cannot filter, must irradiate and use CMV-negative

Front 84

granulocyte storage

Back 84

24 hours within collection 20-24 c without agitation

Front 85

DDAVP action

Back 85

release of vwf/factor 8 from endothelial cells

Front 86

ddavp in uremic thrombocytopathy

Back 86

0.3 ug/kg IV
BEFORE platelets or cryo

Front 87

uses of ddavp

Back 87

1) uremic thrombocytopathy 2) vwf 3) mild hemo A 4) hepatic failure

Front 88

chronic ddavp use

Back 88

tachyphylaxis

Front 89

novoseven uses

Back 89

1) hemo A/B inhibitors 2) congenital factor 7 deficiency

Front 90

half life novoseven

Back 90

2 hrs