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30 Cards in this Set

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1. It is normal to add loading dye to a sample prior to loading it on a gel, it is purchased as a 6X stock. If the sample is 60 uLs and you want to load it all, how much loading dye would you add?

A. 6 uL
B. 10 uLs
C. 12 uLs
D. 15 uLs
C. 12 uLs

60/5 = 12
2. A limitation of capillary electrophoresis is that you can only use low currents for electrophoresis because the large volume of matrix used.

A. True
B. False
B. False
3. Pulse field electrophoresis is often used to separate very large neucleic acid pieces

A. True
B. False
A. True
4. Once a gel has been prepared, loaded, and electrophoresis is complete, it will probably require some type of documentation, this can be accomplished by staining with:

A. Brom cresol blue
B. xylene cyanol
C. Ethidium bromide
D. eosin
C. Ethidium bromide
5. If you wanted to prepare a 2.0% agarose gel that had a bed volume of 200 mLs, you would need:

A. 0.4 grams of agar and 20 mLs of buffer
B. 4.0 grams of agar and 200 mLs of buffer
C. 0.4 grams of agar and 20 mLs of water
D. 4.0 grams of agar and 200 mLs of water
B. 4.0 grams of agar and 200 mLs of buffer

2gms / 100ul
6. If you needed to make a 6% acrylamide gel with a bed volume of 5 mLs and had a 30% acrylamide stock and 50X TAE, you would add:

A. 1 mL aof acrylamide
B. 0.1 mLs of acrylamide
A. 1 mL aof acrylamide

6*5 = 30* ? 30/30=1
7. If you needed to make a 6% acrylamide gel with a bed volume of 5 mLs and had a 30% acrylamide stock and 50X TAE, you would add:

A. 100 uLs of 50X TAE
B. 1 mL of 50X TAE
A. 100 uLs of 50X TAE

1*5=50*? 5/50 = 0.1 ml = 100 uLs
8. Bands of nucleic acids can be recovered from a gel by either electrophroresis or diffusion.

A. True
B. False
A. True
9. Hybridization requires that nucleic acid materials be bound to a solid support, either a membrane, plastic planes, or even plastic beads.

A. True
B. False
A. True
10. For a successful hybridization, the probe and the target DNA must be:

A. neutralized
B. depurinated
C. denatured
D. stained
C. denatured
11. Transferring nucleic acids from a gel to a nylon filter can be done by passing liquid through the gel either passively or actively (+ or – pressure or capillary action).

A. True
B. False
A. True
12. Once transferred to a membrane, the DNA needs to be immobilized and that can be accomplished by either baking or exposure to UV light (irradiant energy).

A. True
B. False
A. True
13. In hydridization based assay, high molecular weight substances like milk protein, albumin, ficol, or sheared salmon sperm DNA are used for blocking specific binding of the probe.

A. True
B. False
B. False

not specific
14. The specificity and sensitivity of a hybridization assay can be affected by adjusting the temperature used for hybridization and subsequent stringency washes as well as the Na+ concentration of the buffers used.

A. True
B. False
A. True
15. If this were an autosomal recessive trait, who is not a carrier?
A. child 1
B. child 2
C. child 3
D. nobody
B. child 2
16. If this were an autosomal recessive trait, who probably has the disease?
A. child 1
B. child 2
C. child 3
D. nobody
A. child 1
17. What are the chances that the parents will have another child that exhibits disease?
A. 25%
B. 50%
C. 100%
A. 25%
18. Amplification assays that are PCR based require which of the following component besides primers that flank the area of interest, buffer, dNTP’s, and Taq polymerase.
A. Ca++
B. Template
C. restriction endonuclease
B. Template
19. Amplification assays consume all of the following components of the rxn except for:
A. primers
B. dNTP’s
C. template/DNA
C. template/DNA
20. If the primers of a PCR rxn flank a 100 bp segment of template and the primers are 20 bps each, what is the expected size of the amplified product:
A. 100 bps
B. 120 bps
C. 140 bps
C. 140

20+100+20 = 140
21. Which of the following oligonucleotides can bind to this single stranded template:

5’-ACCGTTAACCGGTTTTATATC-3’

A. 5’-TTGGCCAA
B. 5’-AACCGGTT
B. 5’-AACCGGTT
~~~~~~~~~~~~~~~~~~~~~~
5’-ACCGTT**TTATATC-3’

*AACCGGTT*
3'-TTGGCCAA-5"
22. Which of the following oligonucleotides sequences has the higher Tm if double stranded

A. 5’-GCGCGCGCGCGC B. 5’-ATATATATATATATAT
A. 5’-GCGCGCGCGCGC
~~~~~~~~~~~~~~~
A has 12*4 = 48
B has 16*2 = 32
23. Secondary structures of DNA are highly stable and are not influenced by salt concentrations nor temperature variation

A. True
B. False
B. False
24. RNA:
A. is single stranded
B. is double stranded
C. is highly stable outside the cell
A. is single stranded
25. When DNA is isolated from a cell, both nuclear and mitochondrial DNA are recovered.

A. True
B. False
A. True
26. An exon is the part of the mammalian gene translated into protein:

A. True
B. False
A. True
27. The closer two genes are on a chromosome, the less likely there will be a recombination event.

A. True
B. False
B. False
28. If you had a O.D. reading of 0.060 on a sample and that was obtained after making a 1/200 dilution of a stock of DNA, how many ugs/ul of DNA does the stock have?

A. 0.003
B. 0.060
C. 0.120
D. 0.600
D. 0.600
~~~~~~~~~~~~~~~~~~
0.06*50*200/1000 = 0.06

0.06*10 = 0.600
29. Given the following O.D. reading, what statement would reflect the utility of the DNA from this isolation?

O.D.260 – 0.050
O.D.280 – 0.025

A. the material is not of sufficient molecular mass and the isolation should be repeated

B. the purity is within acceptable range and the materials should perform well

C. the purity is not within acceptable range and the isolation should be repeated

D. the material is of adequate size and should perform well
B. the purity is within acceptable range and the materials should perform well

~~~~~~~~~
0.050/0.025 = 2.0
30. From the isolated sample you got the following O.D. readings:

A260 = 0.070
A280 = 0.035

What is the purity of the sample?
A. can’t be determined
B. 2.0
C. 0.5
B. 2.0
~~~~~~~~~~
0.070/0.035 = 2.0