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20 Cards in this Set
- Front
- Back
immunity
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resistance of an organism to infection or disease
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immunization
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initial contact
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antigens
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factors capable of eliciting an immune response
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antibody
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protein formed in response to a challenge of the immune system by a foreign agent; bind to specific antigens
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epitope
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shape on antigen that antibody recognizes and binds to
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serum (plural sera)
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clear fluid obtained when the solid components are removed from whole clotted blood
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humanized antibody
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a human antibody thats been engineered to contain some of the antigen binding region of a mouse monoclonal antibody
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Ouchterlony or double diffusion assays
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test that uses antibodies and antigen in wells tht both diffuse toward each other, if they interact, they will form a precipitate indicating recognition
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primary antibody
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an antibody that recognizes an antigen of interest
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secondary antibody
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an additional antibody that recongizes all primary antibodies of one type derived from one species
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IgA
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first line of defense against invading microorganisms; found in tears, salva, milk and mucous secretions of respiratory, genital and intestinal tracts
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IgD
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initiates B cell activation
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IgE
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in allergic reactions; binds to allergens and stimulates histamine release
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IgM
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found in serum and is responsible for primary immune response
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immunoassays
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are tests that exploit the specificity with which antibodies bind molecules to detect or measure those molecules in biological samples
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Enzyme- Linked Immunosorbent Assay (ELISA)
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uses antibodies to detect an antigen or antibody of interest by exploiting tendency of proteins to absorb
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Western Blotting
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can provide information about proteins size and abundance in sample
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electroblotting
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transfers proteins from gel to membrane
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Multiplex Bead Assays
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use microscopic beads to provide quantitative measurements of large numbers of analytes
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fluorescence activated cell sorting
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high throughput assay that sorts cells into different populations based on presence of antigens on cell surfaces
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