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20 Cards in this Set

  • Front
  • Back
immunity
resistance of an organism to infection or disease
immunization
initial contact
antigens
factors capable of eliciting an immune response
antibody
protein formed in response to a challenge of the immune system by a foreign agent; bind to specific antigens
epitope
shape on antigen that antibody recognizes and binds to
serum (plural sera)
clear fluid obtained when the solid components are removed from whole clotted blood
humanized antibody
a human antibody thats been engineered to contain some of the antigen binding region of a mouse monoclonal antibody
Ouchterlony or double diffusion assays
test that uses antibodies and antigen in wells tht both diffuse toward each other, if they interact, they will form a precipitate indicating recognition
primary antibody
an antibody that recognizes an antigen of interest
secondary antibody
an additional antibody that recongizes all primary antibodies of one type derived from one species
IgA
first line of defense against invading microorganisms; found in tears, salva, milk and mucous secretions of respiratory, genital and intestinal tracts
IgD
initiates B cell activation
IgE
in allergic reactions; binds to allergens and stimulates histamine release
IgM
found in serum and is responsible for primary immune response
immunoassays
are tests that exploit the specificity with which antibodies bind molecules to detect or measure those molecules in biological samples
Enzyme- Linked Immunosorbent Assay (ELISA)
uses antibodies to detect an antigen or antibody of interest by exploiting tendency of proteins to absorb
Western Blotting
can provide information about proteins size and abundance in sample
electroblotting
transfers proteins from gel to membrane
Multiplex Bead Assays
use microscopic beads to provide quantitative measurements of large numbers of analytes
fluorescence activated cell sorting
high throughput assay that sorts cells into different populations based on presence of antigens on cell surfaces