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156 Cards in this Set

  • Front
  • Back

ALL organisms are made of what?

Cells.

The cell is the simplest/smallest collection of matter that is considered what?

Alive.

ALL cells are related how?

Through there descent from earlier cells.

Evolutionary changes take place how?

Mutation of DNA.

Who was the first person to visualize and document cells through a microscope?

Robert Hooke in 1665.

The first microscopes, and common laboratory microscopes are what?

Light Microscopes, use light refraction to magnify images.

What is magnification?

The ratio of an object's image size to it's real size.

What is resolution?

A measure of clarity of an image.

What is contrast?

The accentuation of differences in parts of an image.

What do Electron Microscopes allow us to view?

At the organelle level and smaller.

How does an Electron Microscope work?

Focuses a beam of electrons through or onto the sample.

What is an advanced version of the Electron Microscope which allows for the viewing of internal structures?

Transmission Electron Microscope.

Why can't live cells be observed using a Electron Microscope?

They are killed in the process of preparation.

What type of microscopy allows for viewing samples in 3D.

Confocal and deconvolution microscopy.

What is cell fractionation?

A method using a centrifuge to separate out cell components based on weight.

What is a pellet?

Heavier organelles and/or components formed after centrifugation.

What is a supernatant?

Liquid and other debris above the pellet.

How are different size particles separated out during centrfugation?

By differing centrifuge speed and solvents containing the samples.

What are the two types of cells?

1) Procaryotic (Bacteria and Archeae)


2) Eucaryotic (all other cells)

What is the plasma barrier?

A bounding, selective barrier around all cells.

ALL cells have what that come in the form of DNA?

Chromosomes.

Where does protein synthesis occur in ALMOST all cells?

Ribosomes.

What is the major difference between eucaryotes and procaryotes?

Where their DNA is stored.

Eucaryotic cells store their DNA where?

In a double membrane bound organelle called the nucleus.

Procaryotic cells store their DNA where?

In a non-membrane bound region known as a nucleoid because they have no nucleus.

What is cytoplasm?

The entire interior of the cell minus the nucleus.

What type of cell is larger than the other?

Eucaryotic > Procaryotic

Larger organisms do not have larger cells but?

More of them.

What is the job of the selective barrier?

Allowing passage for oxygen, nutrients, and wastes.

What are microvilli?

Long thin projections extending out from the surface that increases the surface area of the cell without increasing too much volume.

What are the components of the cytoplasm?

1) Cytosol


2) Organelle


3) Ion


4) Water


5) Proteins


6) Other biomolecules

Eucaryotic cell's genetic instructions are where?

In the nucleus.

What carries out the genetic instructions in a Eukaryote?

The ribosomes.

What is important about the nucleus?

Contains most of the genes (in DNA/chromosomes).

What is the nuclear envelope?

A double membrane enclosing the nucleus that separates the nucleus from the cytoplasm and is perforated by pore structures (nuclear pores).

What is the nuclear lamina?

A network of protein filaments located on the nuclear side that maintains the shape of the nucleus.

What is a chromosome?

Compact structure containing many proteins and one long DNA molecule.

How many chromosomes do humans have?

46

What is a chromatin?

The complex proteins in a chromosome.

What is a nucleolus?

A mass of dense granules in the nucleus where ribosomal RNA (rRNA) and proteins are combined to make ribosomes.

mRNA is made from what?

DNA.

How does mRNA become a final protein product?

Travels through nuclear pores to the rough endoplasmic reticulum where it is translated into a polypeptide and untimely a final protein product.

What are ribosomes?

Large complexes/groupings that carry out protein synthesis and are made up of rRNA and proteins (protein factories).

What are free ribosomes?

They circulate in the cytoplasm and are where protein synthesis starts due to the synthesizing of enzymes and proteins destined for the bound ribosomes.

What are bound ribosomes?

They are attached to the endoplasmic reticulum.

What do bound ribosomes make?

Proteins destined for: insertion unto membranes and packaging within organelles.

What does the endomembrane system regulate?

Protein traffic and performs metabolic function.

What is the endomembrane system?

The entire membrane system within cells.

What does the endomembrane system include?

1) nuclear envelope


2) endoplasmic reticulum


3) Golgi apparatus


4) vesicles


5) plasma membrane

What are vesicles?

Sacs/spheres made of membrane that travel amongst the endomembrane system.

What are transport vesicles?

Vesicles in transit from one part of the cell to another.

What is the endoplasmic reticulum?

An extensive network of membranes where protein synthesis can take place.

What is the cisternae?

A network of membranous tubules and sacs in the endoplasmic reticulum.

What is a lumen?

An internal compartment (cavity) or cisternae space in the endoplasmic reticulum.

Why are the endoplasmic reticulum and nucleus in close proximity to each other?

Because the endoplasmic reticulum is continuous/attached to the nucleus.

What part of the endoplasmic reticulum lacks ribosomes?

Smooth.

What part of the endoplasmic reticulum is studded with ribosomes?

Rough.

What are the four functions of the smooth endoplasmic reticulum?

1) synthesis of lipids


2) metabolism of carbohydrates


3) detoxification of drugs and poisons


4) storage of calcium

How does the rough endoplasmic reticulum function in protein synthesis?

A polypeptide chain grows from a bound ribosome, it extends into the endoplasmic reticulum lumen where it begins the folding process.

What are glycoproteins?

Secretory proteins that have carbohydrates covalently attached to them.

What is the function of the Golgi apparatus?

It is the center for receiving, sorting, shipping, and some manufacturing of proteins.

What are the two sides of the Golgi apparatus?

Cis side (receiving) and trans side (shipping).

What do budding transport vesicles do?

Contain modified protein, leave from the trans side & travel to other parts of the cell or get excreted outside the cell.

What is a lysosome?

A membranous sac of enzymes used to digest macromolecules (digestive compartments).

Where to lysosomes work best?

In an acidic environment.

What is phagocytosis?

The engulfing of smaller organisms or materials by surrounding them and consuming.

What forms from phagocytosis?

Food vacuoles.

What is autophagy?

The recycling of a cell's own materials.

What are vacuoles?

Large vesicles that perform various functions and derive from the ER and Golgi (diverse maintenance compartments).

What is a contractile vacuole?

Pumps excess water out of a protist cell.

What is a central vacuole?

Contain the sap of a plant cell.

What do mitochondria and chloroplasts do?

Transforms energy into energy that am organism can use.

What is cellular respiration?

The metabolic process that converts sugars, fats, and oxygen into the energy molecule ATP.

What is mitochondria?

The site of cellular respiration.

What is a chloroplast?

The site of photosynthesis in plants and algae where solar energy is converted into chemical energy.

What is the Endosymbiont Theory?

Mitochondria and chloroplasts share many similarities with bacteria. States that an early ancestor of eukaryotic cells engulfed an ancestral procaryote and set up a symbiotic relationship that eventually lead the the creation of mitochondria in one case and chloroplasts in another.

Evidence to support Endosymbiont Theory?

1) they have a double membrane


2) they have their own ribosome and DNA


3) they seem to exist independently of the cell.

What is found in NEARLY all eukaryotic cells and number in the hundreds to thousands depending on the amount of cellular respiration occurring?

Mitochondria.

What is mitochondria cistae?

Infoldings (villi-like) found on the inner membrane of the mitochondria's double membrane.

What is the intermembrane space?

First of two internal compartments found in mitochondria.

What is the mitochondrial matrix?

Second internal compartment of the mitochondria containing enzymes, DNA, and ribosomes.

Where is cellular respiration carried out?

In the mitochondrial matrix.

Chloroplasts are only found where and carry out what process?

Plants, photosynthesis.

What is a thylakoid?

A membranous system within the chloroplast consisting of flattened sacs.

What is a granum?

A stack of thylakoid.

What is stroma?

The fluid outside of the thylakoids.

What are the three parts of chloroplast?

1) intermembrane space


2) stroma


3) thylakoid space

What are plastids?

A family of closely related plant organelles.

What are the three plastids?

1) chloroplast


2) amyloplast


3) chromoplast

What is an amyloplast?

Colorless organelle that stores starch.

What are chromoplasts?

Organelles containing pigments that give color.

What do Peroxisomes do?

Transfers hydrogen atoms into oxygen to form hydrogen peroxide through the use of special enzymes.

What are Glyoxysomes?

Specialized peroxisomes found in fat-storing tissues of plants.

What is the Cytoskeleton?

A network of fibers throughout the cytoplasm that organizes structures and activities in the cell.

What are three main structures in the Cytoskeleton?

1) Microtubules


2) Microfilamants


3) Intermediate Filaments



What is the role of the Cytoskeleton?

Provides mechanical support to the cell wall and maintains its shape.

What is a cytoskeleton so important in animals?

Because animals lack a strong cell wall.

What is cell motility?

Aides in changes in cell location and more limited movements of parts of the cell.

What are motor proteins?

Proteins attached to the cytoskeleton and use ATP to power movement.

How is movement generated?

Proteins moving along the cytoskeletal fibers.

What cytoskeletal structure is the thickest?

Microtubules.

What cytoskeletal structure is the thinnest?

Microfilaments; also called actin filaments.

What is the size of intermediate filaments?

Size of diameters is between microtubules and microfilaments.

What are the dimensions of hollow rods?

25nm diameter and 200-25,000nm in length

What is a Tubulin?

A globular protein composing the wall of microtubules.

Each tubulin protein consists of what?

A alpha and beta tubulin.

Microtubules grow how?

Adding tubulin dimers.

What do microtubules do?

Guide secretory vesicles from the Golgi to the plasma membrane.

What are Centrosomes?

Region located near the nucleus that organizes microtubules for chromosomal separation during mitosis

What are Centrioles?

Found in pairs and are composed of 9 sets of triplet microtubules that help assemble and organize microtubules during mitosis.

What are flagella?

Microtubule containing projections that allows for cell movement, limited to just a few per cell.

What are Cilia?

Microtubule containing projections that allows for cell movement, usually number in the hundreds per cell.

What beating pattern do flagella and cilia use?

"9+2"

What pattern in some cilia is considered nonmotile?

"9+0"

What is the basal body?

Anchors the microtubule to the cell wall.

What are Dyneins?

Large motor proteins responsible for the bending movements of an organelle, utilizes ATP as their energy source.

What are microfilament diameters?

Solid rods about 7nm diameter.

What is actin?

A globular protein that is the building blocks for microfilaments.

What is the shape of a microfilament?

A twisted double chain of actin subunits.

What is the purpose of microfilaments?

To bear tension or pulling forces.

What is the Cortex?

The outermost cytoplasmic layer of a cell.

What are Microvilli?

Delicate projects on a cell surface made up of bundles of microfilaments.

What important role do microfilaments play in?

Muscle contraction.

What is Myosin?

Motor protein in muscle cells that walk along the bundles of microfilaments of the muscle tissue.

Contraction of muscle results from what?

Actin and Myosin sliding past one another shortening the muscle.

What are Pseudopodia?

Cellular extensions/projections that allow for the movement of organisms like the amoeba.

What is Cytoplasmic Streaming?

The circular flow of the cytoplasm that speeds the distribution of materials within the cell.

What is the diameter of intermediate filaments?

8-12nm

Intermediate filaments are related to microfilaments how?

Specialized for bearing tension.

What are intermediate filaments constructed from?

Keratins.

What can leave behind a network of intermediate filaments?

Chemical treatments.

What major role does intermediate filaments play?

Reinforcing cell shape and fixing the position of certain organelles.

What are axons reinforced by?

Microfilaments.

What helps coordinate cellular activities?

Extracellular components and connections between cells.

What is a Cell Wall?

An extracellular structure of plant cells that distinguish them from animal cells.

What are the three functions of the cell wall?

1) Protects the plant cell


2) Maintains the cell shape


3) Prevents excessive uptake of water

What is cellulose synthase?

An enzyme that combines cellulose and microfibrils.

What is the Primary Cell Wall?

Thin and flexible cell wall of young plants.

What is the Middle Lamella?

A thin layer rich in sticky polysaccharides that forms between the primary walls of adjacent plant cells.

What is Pectin?

Sticky polysaccharides of the middle lamella that glues adjacent cells together.

What is the Secondary Cell Wall?

Deposited in several layers between the primary cell wall and the plant cell membrane.

What are the main components of Extracellular Matrix in animals?

Glycoproteins and other carbohydrate containing molecules.

What is the most abundant glycoprotein in the ECM?

Collagen.

What are two characteristics of collagen?

They form strong fibers outside the cell and accounts for 40% of the total protein in the human body.

What do Proteoglycans do?

Form the network or lattice of molecules in which collagen fibers get embedded to make a solid structure.

What is Fibronectin?

Another glycoprotein (similar to protein) that attaches cells to the ECM.

What are Integrins?

Cell-surface receptors that bind to the ECM and fibronectin.

What role does the Integrins have?

Transmit signals between the ECM and cytoskeleton.

What are signaling pathways?

Combination of mechanical and chemical signals.

What are Plasmodesmata?

Membrane-lined channels filled with cytoplasm.

How to proteins reach the plasmodesmata?

By moving along the fibers of the cytoskeleton.

What are cell junctions?

Connection points between adjacent cells?

What are the three types of cell junctions?

1) Tight junctions


2) Desmosomes


3) Gap junctions

What are Tight junctions?

Portions of the plasma membrane of adjacent cells are tightly pressed against each other forming a continuous seals around the cell.

What are Desmosomes?

Function like rivets; intermediate filaments made of keratin protein anchor desmosomes in the cytoplasm.

What are Gap junctions?

Provide cytoplasmic channels to adjacent cells consisting of membrane proteins surrounding a pore.