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65 Cards in this Set
- Front
- Back
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Starch Hydrolysis
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Starch agar
iodine clear growth + dark blue - |
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Lipid Hydrolysis
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Tributyrin agar
none clear growth+ opaque- |
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Casein Hydrolysis
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skim milk agar
none clear+ opaque- |
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Gelatin
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Gelatin
none liquid + solid - |
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Carbohydrate Fermentation acid
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Phenyl Red Carbohydrate Broth
none yellow + red - |
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Carbohydrate Fermentation gas
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Phenyl red carb broth
durham tube bubbles no bubbles |
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Oxidase test
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loopful of agar
oxidase reagent purple + clear - |
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Indole
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SIM
kovacs reagent red + yellow - |
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methyl red
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mrvp broth
MR red + yellow - |
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Voges Proskauer
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MRVP Broth & incubation
barrits reagent a+b red + clear - |
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Citrate utilization test
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simmons citrate agar
none prussian blue green - |
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hydrogen sulfide production
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SIM/TSI
none black + clear - |
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Motility test
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SIM
none cloudy throughout cloudy at top - |
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Phenylalanine Deamination
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phenylalanine agar
ferric chloride green at top clear agar - |
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nitrate reductase
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nitrate broth
reagent a+b and minute c red after a+b red after c |
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MSA detects, example, and agar characteristics
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staphlococcus, s. aureus, high salt concentration & pH indicator
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DNase test, what made up of, what identifies, what it shows, and positive result
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made up of DNA
identify s. aureus shows if can hydrolyze DNA pink + |
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novobiocin test s. epi & s. sapro
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staph edipdermidis is sensitive
and staph sapro resistant |
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s. pyogenes
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strep & scarlet fever
group A strep lancefield group |
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bacitracin test
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beta hemolytic strep identify
filter paper on top of blood agar plate, zone of inhibition means streptococci group a present if not non group a present |
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what is sensitive to bacitracin test
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group a strep
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bile esculin agar
+/- identify |
identify group d strep
dark black + none - non group d |
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SF broth/6.5% NaCl broth
+/- identify |
purple broth
identify E. faecalis + brown/yellow -purple |
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optochin test/p test
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differentiate s. pneumonia from alpha hemolytic strep
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catalase test
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differentiate streptococcus (-) from staphlococcus (+)
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Enterotube
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biochemical test for enteric organism
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4 genera of enteric baciili & two traits they share in common
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salmonella, shigella, e. coli, klebsiellia (all gram - rods & intestinal)
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IMViC stands for and used to identify
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indole, methyl red, voges proskaeur, citrate all identify enterobacilli
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SIM stands for
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sulfide indole motility
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two morphological characterisitics of fungi
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yeast & mycelium/hyphae
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fungi ideal temperature, incubation period, pH
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25 degrees
extended incubation 4.5 to 5.6 pH |
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Taenia & Fluke
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Taenia tapeworm rt in light blue box and fluke left in lt blue box
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trypanosoma
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african sleeping sickness & shagas
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toxoplasma
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toxoplasmosis
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entamoeba
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dysentary
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balantidium
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dysentary
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giardia
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giardiases
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trichomonas vaginalis
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vaginitis
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trichonella spiralis
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trichonosis
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http://www.pathology.vcu.edu/education/cardio/images/2e.c.jpg
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beta hemolysis
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http://medinfo.ufl.edu/year2/mmid/bms5300/images/d7080.jpg
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beta hemolysis
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http://www.mgm.ufl.edu/~gulig/mmid/mmid-lab/labimage/s_pnc.jpg
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alpha hemolysis
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http://www.mc.maricopa.edu/~johnson/labtools/Dbiochem/banh1.jpg
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gamma hemolysis
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http://www.jlindquist.net/generalmicro/DMimages/amylase.jpg
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starch agar
+ then - |
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http://microbiology.scu.edu.tw/micro/microbe-exp/exp_website/images/lipid_hydrolysis1.jpg
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lipid trybutyrin agar
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http://microbiology.scu.edu.tw/micro/microbe-exp/exp_website/images/carbohydrate_fermentation.jpg
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carbohydrate fermentation
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http://a-s.clayton.edu/furlong/BIOL2250LAB/Reviews/pictures/tsi.jpg
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The tube on the far left is uninoculated. The second tube shows a yellow butt and a yellow slant. This means the organism fermented glucose and lactose and/or sucrose. The third tube has a yellow slant and a yellow butt. This means that the organism fermented glucose and lactose and/or sucrose and reduced sulfate. The fourth tube from the left shows a yellow butt and a red slant. This means that the organism only fermented glucose.
The last tube shows zero fermentation, but shows a positive result for sulfate reduction. |
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http://microbiology.scu.edu.tw/micro/microbe-exp/exp_website/images/sim-1.jpg
first three |
indole test
1st not inoculated positive negative |
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http://microbiology.scu.edu.tw/micro/microbe-exp/exp_website/images/vp-1.jpg
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vp
uninoculated red positive yellow negative |
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http://www.pathology.vcu.edu/education/cardio/images/2e.c.jpg
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beta hemolysis
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http://medinfo.ufl.edu/year2/mmid/bms5300/images/d7080.jpg
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beta hemolysis
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http://www.mgm.ufl.edu/~gulig/mmid/mmid-lab/labimage/s_pnc.jpg
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alpha hemolysis
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http://www.mc.maricopa.edu/~johnson/labtools/Dbiochem/banh1.jpg
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gamma hemolysis
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http://www.jlindquist.net/generalmicro/DMimages/amylase.jpg
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starch agar
+ then - |
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http://microbiology.scu.edu.tw/micro/microbe-exp/exp_website/images/lipid_hydrolysis1.jpg
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lipid trybutyrin agar
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http://microbiology.scu.edu.tw/micro/microbe-exp/exp_website/images/carbohydrate_fermentation.jpg
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carbohydrate fermentation
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http://a-s.clayton.edu/furlong/BIOL2250LAB/Reviews/pictures/tsi.jpg
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The tube on the far left is uninoculated. The second tube shows a yellow butt and a yellow slant. This means the organism fermented glucose and lactose and/or sucrose. The third tube has a yellow slant and a yellow butt. This means that the organism fermented glucose and lactose and/or sucrose and reduced sulfate. The fourth tube from the left shows a yellow butt and a red slant. This means that the organism only fermented glucose.
The last tube shows zero fermentation, but shows a positive result for sulfate reduction. |
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http://microbiology.scu.edu.tw/micro/microbe-exp/exp_website/images/sim-1.jpg
first three |
indole test
1st not inoculated positive negative |
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http://microbiology.scu.edu.tw/micro/microbe-exp/exp_website/images/vp-1.jpg
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voges proskauer
uninoculated red positive yellow negative |
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http://www.mc.maricopa.edu/~johnson/labtools/Dbiochem/cita.jpg
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citrate utilization test
green negative blue positive |
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http://faculty.ntcc.edu/mhearron/SIM-sulfur_.JPG
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hydrogen sulfide production
yellow negative black positive with motility yellow w black line positive w no motility |
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http://biosci.usc.edu/courses/2002-fall/documents/bisc300-lab_NitrateReduction.jpg
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nitrate
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http://mail.scu.edu.tw/~92134001/Image7.gif
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oxidase test
purple positive clear negative |
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http://users.stlcc.edu/kkiser/BBE.JPG
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bile esculin agar positive black
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http://www.life.umd.edu/classroom/bsci424/Images/PathogenImages/A-Disk(Bacitracin)RxnsforGrpABeta-HemoStrep.jpg
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bacitracin test
positive yellow group a strep negative red no growth |
