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42 Cards in this Set
- Front
- Back
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Lab 9: Protein Purification and Chromatography
What digests the bacterial cell wall? |
lysozyme
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Lab 9: Protein Purification and Chromatography
The freezing and thawing process causes the bacteria to do what? |
Explode and rupture completely
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Lab 9: Protein Purification and Chromatography
Why is it important to add the EB (equilibration buffer) to the side of the upper plastic wall of the column? |
You do not want to disturb the powdery bead surface.
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Lab 9: Protein Purification and Chromatography
What is the prediction for the sample in the binding buffer (very high salt)? |
No bacteria will get washed out and no GFP will get through the beads.
In column; slight fluorescence In collection tube; nothing |
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Lab 9: Protein Purification and Chromatography
What is the prediction for the sample with the wash buffer? |
Because GFP is so hydrophobic it will not get washed out; hydrophilic bacterial proteins will get washed out
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Lab 9: Protein Purification and Chromatography
What is the prediction for the sample with the elution buffer? |
GFP will get washed out so the tube should be fluorescing.
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Lab 9: Protein Purification and Chromatography
What was the purpose of rupturing or lysing the bacteria? |
Complete disruption releases soluble components including GFP
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Lab 9: Protein Purification and Chromatography
Name the buffer: The high salt buffer |
The equilibration buffer (EB)
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Lab 9: Protein Purification and Chromatography
Name the buffer: A very high salt buffer |
Binding buffer (BB)
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Lab 9: Protein Purification and Chromatography
Name the buffer: A medium salt buffer |
The wash buffer (WB)
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Lab 9: Protein Purification and Chromatography
Name the buffer: A very low salt buffer |
Elution buffer (TE)
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Lab 10: Reproduction
What solution was used to encourage the sea urchin to release its gametes? |
KCl
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Lab 10: Reproduction
Immediately after the egg is fertilized a what forms around the zygote? |
A fertilization membrane
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Lab 10: Reproduction
What makes up the pistil? |
The stigma, the style, and the ovary
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Lab 10: Reproduction
What makes up the stamen? |
The anther and the filament
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Lab 10: Reproduction
What is the purpose of the stigma? |
It collects pollen
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Lab 10: Reproduction
What is the purpose of the style? |
holds stigma up
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Lab 10: Reproduction
Petals are key in doing what? |
attracting pollinators
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Lab 10: Reproduction
A pollen grain matures quickly, germinates, and forms a: |
pollen tube
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Lab 10: Reproduction
2 sperm nuclei migrate through the pollen tube, and what takes place? |
Double fertilization
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Lab 10: Reproduction
What are the 2 steps of double fertilization? |
1. one sperm nucleus fuses with the egg to produce the diploid (2n) zygote
2. one sperm nucleus fuses with the 2 polar nuclei to produce the triploid (3n) endosperm fusion nucleus |
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Lab 10: Reproduction
What is the food storage tissue for the embryo? |
endosperm
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See page 147 for picture and labeling of maturing plant embryo.
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147
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Lab 10: Reproduction
The corn seed is an example of what? |
a monocot
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Lab 10: Reproduction
The bean seed is an example of what? |
a dicot
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Lab 11: Reproduction
Put these stages of development in frogs in order. 4 cells neurula early neurula tadpole larva 4-8 cells blastula |
4 cells
4-8 cells blastula early neurula neurula tadpole larva |
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Lab 11: Reproduction
The intestines of the frog became relatively _______ with more and more development. |
smaller
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Lab 11: Reproduction
The following will develop into what tissues in plants? protoderm |
epidermal tissue
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Lab 11: Reproduction
The following will develop into what tissues in plants? procambium |
vascular cells
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Lab 11: Reproduction
The following will develop into what tissues in plants? bud primordia |
lateral primodia
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Lab 11: Reproduction
The following will develop into what tissues in plants? leaf primordia |
leaves
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See page 165 for labeling of figure (plant shoot and apical bud).
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165
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Old labs:
SEM (Scanning Electron Microscopy) is used for: |
3D - larger objects, lower resolution
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Old labs:
TEM (transmission electron microscopy) is used for: |
2D - smaller objects, higher resolution
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Old labs:
bacillus cereus has what shape? |
rod-shaped
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Old labs:
In differential staining, if the cells stain purple, they are classified as: |
Gram-positive
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Old labs:
In differential staining, if the cells stain red, they are classified as: |
Gram-negative
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Old labs:
What was the purpose of this test? egg albumin acted as positive control, water was negative control |
testing for presence of protein
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Old labs:
In the test for the presence of protein, milk tested _________, because it turned purple. |
positive
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Old labs:
How did we test the activity of enzyme catalase under various conditions? |
time it took the filter disc to float back to the surface of the solution
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Old labs:
Why did the boiled cypridina not generate light? |
the luciferase enzyme had been denatured and therefore deactivated
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Old labs:
Name the part on the compound microscope. |
Coarse focus
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