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15 Cards in this Set
- Front
- Back
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what are restriction endonucleases?
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enzymes that recognize specific nucleotide sequences (restriction sites), and cleave dna creating dna fragments
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what is type 1?
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-simple cuts across both strands
-not often used |
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what is type 2?
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-allows physical mapping
-allows recombinant molecules |
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what is the most common and flexible host?
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E. coli
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what are plasmids?
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small, extrachromosomal dnas dispensable to the cell, 10kb
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what are phages?
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viruses that infect bacterial cells, 40kb
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what is a multiple cloning site(mcs)?
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a region w/unique restriction sites that, when cut, will produce a linear plasmid
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what is recombinant dna?
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dna created in the lab from pieces of different genomes.
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what is a dna library?
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a collection of dna from a specific source in a form that can be propagated in a host
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what is a genomic library?
what is a cDNA library? |
representation of entire genome in a vector;
is limited to expressed genes isolated by reverse transcriptase |
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what are the four stages of the genectic engineering experiment?
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Stage 1: DNA cleavage
Stage 2: production of recombinant dna stage 3: cloning stage 4: screening |
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what is hybridization?
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cloned genes form base pairs w/ complementary sequences on another nucleic acid (probe)
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what are the three basic steps of the polymerase chain reaction?
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1.) denaturation
2.) anealing of primers 3.) primer extension |
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what is the process of Southern blotting?
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-sample dna cleaved into restriction fragments, & spread apart by gel electrophoresis
-gel blotted w/sheet of nitrocellulose -probe of purified, single-stranded dna poured over sheet -if radioact. probe used, band of radioact. appears where probe hybridized w/complementary fragment |
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what are medical applications w/:
atrial peptides- tissue plasminogen activator- |
high blood pressure and kidney failure;
dissolving blood clots |
