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Chapter 27


Bacteria and Archaea

Concept 27.1


Structural and functional adaptations contribute to prokaryotic success

A key feature of nearly all prokaryotic cells is the

Cell wall

Most bacterial cell walls contain

Peptidoglycan


A polymer composed of modified sugars cross-linked by short polypeptides.


This molecular fabric encloses the entire


bacterium and anchors the molecules that extend from its surface.

Archaeal cell walls contain

A variety of polysaccharides and proteins but


lack peptidoglycan

Gram-positive bacteria

Have a thick wall made of peptidoglycan. The crystal violet enters the cell, where it forms a complex with the iodine in the stain. Too large to pass through the thick cell wall, this complex is not removed by the alcohol rinse. Result: The crystal violet masks the safranin dye.

Gram-negative bacteria

Have a thin layer of peptidoglycan, which is located between the plasma membrane and an outer membrane that contains


lipopolysaccharides. The crystal violet-iodine complex can pass through this thin cell wall and hence is removed by the alcohol rinse. Result: The safranin dye stains the cell pick or red.

Gram-negative bacteria



Gram-positive bacteria

The cell wall of many prokaryotes is surrounded by a sticky layer of polysaccharide or protein. This layer is called a

Capsule, if it is dense and well defined, or a slime layer if it is no as well organized. Both kinds of sticky outer layers enable prokaryotes to adhere to their substrate or to other individuals in a colony. Some capsules and slime layers protect against dehydration, and some shield


pathogenic prokaryotes from attacks by their host's immune system.

In another way of withstanding harsh conditions, certain bacteria develop resistant cell called ___


when they lack an essential nutrient

Endospores

Some prokaryotes stick to their substrates or to one another by means of

Fimbriae
Hairlike appendages













Fimbriae


Hairlike appendages









Pili or Sex pili

Appendages that pull two cells together prior to DNA transfer from one cell to the other

Appendages that pull two cells together prior to DNA transfer from one cell to the other

Taxis

A directed movement toward or away from a stimulus


For example, chemotaxis is a directed movement in response to a chemical

Of the various structures that enable prokaryotes to move, the most common are

Flagella

Prokaryotic flagella differ greatly from eukaryotic flagella

Prokaryotic flagella are one-tenth the width and typically are not covered by an extension of the plasma membrane. The flagella or prokaryotes and eukaryotes also differ in their molecular compositions and their mechanism of


propulsion.

Internal Organization and DNA:


Bacteria don’t have mitochondria or


chloroplasts, but still need to metabolize

Some prokaryotic cells have specialized membranes that perform metabolic functions. These membranes are usually infoldings of the plasma membrane.

Prokaryotes have ___ chromosomes

Circular

Since prokaryotes lack a nucleus, their


chromosome is located in the

Nucleoid


A region of the cytoplasm that is not enclosed by a membrane

Plasmids

Smaller rings of DNA

Three factors that contribute to the vast genetic diversity of prokaryotes.

1. Rapid reproduction


2. Mutation


3. Genetic recombination





Prokaryotes can reproduce quickly in favorable conditions by

Binary fission

Mutation

Due to insertions, deletions and base-pair


substitutions in DNA, some offspring may differ genetically.


New mutations, though are on a per gene basis, can increase genetic diversity quickly in species with short generation times and large


populations.