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23 Cards in this Set

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_______________ is the property of some compounds that they absorb light at some wavelength and shortly thereafter emit light at a slightly longer wavelength.
fluorescence
In the lab, the fluorescence microscopes we use are said to be epi-fluorescence microscopes, because the illumination reaches the specimen through the __________ lens.
objective
________________ immunofluorescence is the technique in which the fluorochrome is attached to the antibody directed against the molecule of interest.
direct
________________ immunofluorescence is the technique in which the fluorochrome is attached to a "secondary" antibody directed against the "primary" antibody.
indirect
The __________ filter is used to insure that only the light coming from the excited fluorochrome reaches the observer (or the camera).
emission
The __________ filter is used to insure that the only the light appropriate for excitation of fluorochrome reaches the specimen.
excitation
The use of antibodies in fluorescence is the technique known as ___________________.
Immunofluorescence
To visualize the binding of a rabbit primary antibody, one would use a fluorochrome-conjugated anti-___________.
rabbit_secondary
To visualize the binding of a mouse monoclonal antibody, one could use a fluorochrome-conjugated anti-____________.
mouse_secondary
To visualize the DNA in cells one can use a fluorescent compound known as _________
DAPI
In the microscope to be used for fluorescence, the filters necessary to observe each individual fluorochrome are located in a small box known as a
___filter_____ ____cube_____.
A common red fluorochrome used in biology is __________.
rhodamine, propidium iodide
A common green fluorochrome used in biology is ___________.
fluorescein_(FITC)
The purpose of the dichroic (or mirror) in the fluorescence microscope is to _________ wavelengths of light that excite the fluorochrome.
reflect
The purpose of the dichroic in the fluorescence microscope is to ____________ wavelengths of light that are emitted by the fluorochrome.
transmit
The L8 cells to be used in lab are derived from _______ (species of animal).
mammals
The L8 cells to be used in lab can make what kind of tissue? __________
muscle fiber
A paramecium is an example of what kind of organism? ____________
protist
Elodea comes from what kingdom? __________
plantae
In transmission fluorescence microscopes, there is no dichroic because the light exciting the fluorochrome reaches the specimen by passing through the _______ lens.
condenser
The reason an object appears red in a fluorescence microscope is because the object emits _______ light.
Red
An object will appear blue in the fluorescence microscope if it emits ________ light.
blue
To change the fluorochrome in use in an epi-fluorescence microscope, one changes the
emission___ _____filter____ (two words).