Biol 1101K Lab Practical

Front 1

Major groups of lipids

Back 1

Saturated, unsaturated, steroids, phospholipids, and wax.

Front 2

Major groups of carbohydrates

Back 2

Starch, Glycogen, Cellulose, and Chitin.

Front 3

Saturated vs unsaturated lipids

Back 3

Saturated fat has no double bonds between carbon atoms. mono-unsaturated fat has one double bond between carbon atoms. Polyunsaturated has two or more double bonds between carbon atoms.

Front 4

Solute

Back 4

The substance being dissolved by a solvent to form a solution.

Front 5

Hypotonic

Back 5

A solution that has low concentration of solutes compared to that of the solution placed adjacent to it. Syrup in water.

Front 6

Hypertonic

Back 6

A solution that has a high concentration of solutes relative to an adjacent solution. Water in syrup.

Front 7

Scientific Method Steps

Back 7

Make an observation, ask a question, hypothesis, experiment/test hypothesis, analy

Front 8

Hypothesis

Back 8

A hypothesis is a
proposed explanation or answer to your question based on an educated/ informed guess.

Front 9

Diffusion

Back 9

n is the movement of molecules from a region of
higher concentration to a region of lower concentration

Front 10

Control

Back 10

controls are set up the exact same as your experimental test, but with only one thing different

Front 11

Test for monosaccharides (glucose) and disaccharides (lactos

Back 11

Benedict's reagent. Positive results rage from yellow to orange to red depending of concentration.

Front 12

Test for polysaccharides (starch)

Back 12

Lugol’s solution or Iodine-Potassium Iodide (IKI). Turns dark blue or black in presence of starch.

Front 13

Osmosis

Back 13

; water molecules move down a concentration gradient across a
membrane

Front 14

Test for lipids

Back 14

Sudan red forms a separate red layer in the presence of lipids. Negative turn solution pink.

Front 15

Test for proteins

Back 15

Biuret reagent. Changes form blue to violet in the presence of proteins.

Front 16

Storing microscope

Back 16

1. Rotate the 4X objective into place.
2. Remove the slide from the stage.
3. Slide the light intensity lever to 0.
4. Turn off the power.
5. Unplug the cord and wrap it around the base of the microscope.
6. Replace the dust cover.
7. Use two hands, one under the base, to return the microscope to the cabine

Front 17

Calculating magnification

Back 17

Total magnification = Power of objective lens (4X, 10X, 40X) x Power of ocular lens (usually
10X

Front 18

Muscular system

Back 18

Movement

Front 19

PCR, or Polymerase Chain Reaction

Back 19

A method that creates billions of copies of a specific DNA section can be made from a single fragment, thus vastly increasing the quantity of DNA material
available to be analyzed

Front 20

Digestive system

Back 20

Functions as a path for eaten food to travel and be absorbed by the organism

Front 21

Respiratory system

Back 21

Facilitates breathing functions

Front 22

Circulatory system

Back 22

Facilitates blood movement throughout
the body

Front 23

Excretory system

Back 23

Filters the blood and removes
contaminants or excesses from the body

Front 24

Reproductive system

Back 24

Determines male or female; functions to produce offspring.

Front 25

Skeletal system

Back 25

Physical support for movement

Front 26

Nervous system

Back 26

Communication and control over body functions.

Front 27

Stained gram negative bacteria

Back 27

pink. Gram negative has a phospholipid outer wall.

Front 28

Stained gram positive

Back 28

purple. Gram positive has an outer carbohydrate cell wall.

Front 29

Dna extraction

Back 29

Lysis, purification, precipitation. lysis-heat, Purification-detergent. precipitation-alcohol dehydration of DNA.