Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
15 Cards in this Set
- Front
- Back
|
What is the rate equation?
|
V=k[S]
|
|
|
4 factors that influence enzymes
|
1) pH 2) temperature 3) enzyme concentration 4) substrate concentration
|
|
|
What is the Michaelis-Menton equation?
|
Vo=Vmax[S]/(Km+[S])
|
|
|
What are the three assumptions of the Michaelis-Menton equation?
|
1) The amount of substrate bound by the enzyme at one time is small. 2) [ES] does not change with time. (the steady-state assumption) 3) Only initial reaction rates are used in the analysis.
|
|
|
What is the specificity constant?
|
Vo=(Kcat/Km)[Et][S]
|
|
|
What is the Lineweaver-Burk equation?
|
1/Vo= Km/(Vmax[S]) + 1/Vmax
|
|
|
In competitive inhibition, the inhibitor binds to:
|
The active site of the enzyme.
|
|
|
In uncompetitive inhibition, the inhibitor binds to:
|
a site distinct from the active site and only to the ES complex.
|
|
|
In noncompetitive inhibition, the inhibitor binds to:
|
a site distinct from the active site and to the E or ES complex.
|
|
|
Which class of inhibitors: 1) has no effect of Vmax & 2) Increases Km
|
Competitive
|
|
|
Which class of inhibitors: 1) Decreases Vmax & 2) Has no effect on Km
|
Noncompetitive
|
|
|
When the substrate that binds also causes an allosteric conformational change this is described as:
|
homotropic
|
|
|
When the substrate that binds does not cause an allosteric conformational change, but allosteric conformational change is caused by a different modulator/effector this is described as:
|
heterotropic
|
|
|
In allosteric regulation, instead of using Km, we use a similar term called:
|
K0.5
|
|
|
The competitive inhibitor of Tetrahydrofolate (THF) is:
|
Methotrexate
|
