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23 Cards in this Set
- Front
- Back
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This amino acid transfers ammonia equivalents to the liver for processing into urea
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alanine
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Name the four enzymes used to detect organ damage
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Alanine aminotransferase (ALT)
Aspartate aminotransferase (AST) Lactate dehydrogenase (LD) Creatine Kinase (CK) |
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What is the cofactor required in ALT activity?
plays a critical role in AMINO ACID CATABOLISM |
B6 derivative
pyridoxal 5 phosphate (PLP) |
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Many neurotransmitters require ? for their synthesis
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Pyridoxal 5 phosphate (PLP)
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REMEMBER
use an indicator reaction to measure how fast an enzyme is working ex: NAD --> NADH (increase abs) ex2: NADH --> NAD (decrease abs) |
REMEMBER
use an indicator reaction to measure how fast an enzyme is working ex: NAD --> NADH (increase abs) ex2: NADH --> NAD (decrease abs) |
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What thermodynamic parameter determines reaction rate?
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Activation energy
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What do enzymes ? for chemical reactions and increase kinetics?
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lower the activation barrier
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REMEMBER
[ES] = [E]total [S] / Km + [S] |
REMEMBER
[ES] = [E]total [S] / Km + [S] |
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REMEMBER
V max = K2 [E]total Vo = K2 [ES] |
REMEMBER
V max = K2 [E]total Vo = K2 [S] |
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What is the michaelis menten equation?
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Vo = Vmax [ S ] / Km + [S]
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REMEMBER
Km = k-1 + k-2 / k1 Vmax = K2 [E]total [E]total = [E] + [ES] |
REMEMBER
Km = k-1 + k-2 / k1 Vmax = K2 [E]total [E]total = [E] + [ES] |
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Enzyme concentration correlates with ? in michaelis menten kinectics?
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Vmax
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which michaelis menten parameter depends on substrate concentration?
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Initial Velocity (Vo)
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Which michaelis menten kinetic parametere indicates relative substrate preferences
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Kcat / Km
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REMEMBER!!!!
K2 is synonymous with Kcat |
REMEMBER!!!!
K2 is synonymous with Kcat |
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what is the SPECIFICITY CONSTANT AND REPRESENTS THE ABILITY OF AN ENZYME TO CATALYZE A PARTICULAR SUBSTRATE
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Kcat / Km
K2 / Km |
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The higher the ratio of Kcat to Km (preferred)
IS BETTER than lower ratio Kcat to Km |
The higher the ratio of Kcat to Km (preferred)
IS BETTER than lower ratio Kcat to Km |
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The lower the Km values
how does it relate to activity? |
low km = high activity
high Km = low activity |
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International Unit (IU) is defined as...
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1 μmol / min
AMOUNT of ENZYME that catalyzes the formation of ONE MICROMOLE PRODUCT in ONE MINUTE |
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SI unit is the KATAL (kat)
defined as.. |
1 mol/s
amount of enzyme that catalyzes the formation of ONE MOLE OF PRODUCT IN ONE SECOND |
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REMEMBER
1 Katal = 6 x 10^7 IU |
REMEMBER
1 Katal = 6 x 10^7 IU |
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REMEMBER
ALT NEEDS ITS COFACTOR PLP!!! |
REMEMBER
ALT NEEDS ITS COFACTOR PLP!!! |
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REMEMBER
Vmax / 2 = Km |
REMEMBER
Vmax / 2 = Km |
