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14 Cards in this Set

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DNA replication is (conservative/semi-conservative)

What does this mean?
...semi-conservative.

Each new duplex molecule contains one strand from the parent molecule and one newly synthesized strand.
Replication takes place at a structure called the _____ _____.
...replication fork.
If a replication bubble contains 1 replication fork it's referred to as _____.
...unidirectional.
If a replication bubble contains 2 replication fork it's referred to as _____.
...bidirectional.
DNA replication is (unidirectional/bidirectional).
...bidirectional.
DNA is synthesized in the _____ ---> _____ direction.
5' to 3'
DNAp only has 1 function, and that is to...
...add nucleotides to a growing polypeptide chain.
DNA-directed DNA polymerase:

List 3 characteristics of the way DNAp adds nucleotides to a ppt chain.
1) Adds nucleotides to the 3'-OH of an existing chain.

2) Synthesizes DNA in the 5' ---> 3' direction.

3) Has 3' ---> 5' exonuclase activity (i.e. proofreading)
The term that describes how DNA is transcribed by two different methods (one for each chain) is...
...semi-discontinuous replication
DNA transcription:

The strand that is transcribed continuously is referred to as the...
...leading strand.
DNA transcription:

The strand that is transcribed in discrete fragments is referred to as the...
...lagging strand.
DNAp III adds nucleotides to...
...the 3'-OH of the primer.
Be able to draw strand of DNA being synthesized, making sure each of the following elements are in the drawing:

A) Helicase (unzips)

B) DNAp III (elongates)

C) Primase (synthesizes primer)

D) DNAp I (removes primer; fills gap)

E) Ligase (seals strand)

F) Okazaki fragments

G) Topoisomerase
...okay.
At the macro level (histones), DNA replication begins at a place called the...
...replication origin.