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6 Cards in this Set

  • Front
  • Back
32. size of okazaki fragment
shorter in euks
1-2kb prok
.1-.2" euk ~135bp
29. components of replication fork
1. 2 locally unwound parental strands
2. newly made leading strand
3. newly made lagging strand in short fragments that become ligated later into one continuous strand
33. difference btwn prok/euk WRT origins of replication
1. size of okazaki fragments
2. many polymerases (aplha, beta, gamma, delta, epsilon)
3. mult. origins of replication, bidirectional then fusion of rep fork
similarities RNA polym and DNA replic
1. uses nucleotide triphophates
2. requires DNA template
3. chain growth 5'-3'
4. high fidelity
differences RNA polym and DNA replic
1. no primer req for chain initiation
2. unwinds only local region of DNA
3. RNA displaced as DNA rewinds
4. copies only one strand of DNA
5. disengages from DNA template at specific termination sequences
subunits of RNA polymerase holoenzyme
. σ- promoter recognition and binding; loosely bound; many diff forms
β-chain initiation and elongation
β’- DNA binding
α2-chain initiation, interacts w/ regulatory proteins