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11 Cards in this Set
- Front
- Back
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Is there any difference between protein precipitation and protein denaturation? If there is any, what is it? |
YES! Protein denaturation is loss of function/activity and precipitation is loss of protein solubility. |
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List three chemicals or experimental circumstances, which can cleave disulfide bonds responsible for 3D structure of proteins. |
1. Mercaptoethanol 2. dithiothreitol (DIT) 3. Dithioerythritol (DTE) |
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List three chemicals or experimental circumstances, which can cleave salt linkages responsible for 3D structure of proteins. |
1. Heat 2. extreme pH 3. high salt concentration |
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List three chemicals or experimental circumstances, which can cleave hydrogen bonds responsible for 3D structure of proteins. |
1. Urea 2. extreme pH 3. Heat |
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List three chemicals or experimental circumstances, which can cleave hydrophobic bonds responsible for 3D structure of proteins. |
1. Organic solvents (alcohol, acetane) 2. Urea 3. Detergents |
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What will happen to intracellular proteins after a moderate and an intense heat shock respectively? What kind of proteins/polypeptides are activated in the first and in the second case? |
Moderate: Preserve activity of protein Intense: Loss of function and structure |
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Name th(os)e protein(s) whose misfolding may be associated with Parkinson’s disease. |
alpha-synuclein |
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Name th(os)e protein(s) whose misfolding may be associated with Alzheimer’s disease. |
amyloid-Beta tau |
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Name th(os)e protein(s) whose misfolding may be associated with cystic fibrosis. |
membrane bound protein: cystic fibrosis transmembrane conductance regulator (CFTR) |
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What is the role of the enzyme in the case of enzymatic activity measurement and during enzymatic analysis, respectively? |
In the case of enzymatic activity: the time is important, since umol/min
during enzymatic analysis: time is not important since the determined parameter is the amount of substrate umol |
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Name four enzymatic reactions with direct ammonia release in mammals. |
glutaminase, glutamate dehydrogenase, glycin cleavage enzyme, serin hydratase |
