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20 Cards in this Set
- Front
- Back
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PKA's for Asparate, Gltuatmate, Histidine, Cystine, Tyrosine, Lysine, Arginine |
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Name 6 major enzyme classifications and what they do |
oxidoreductases - Redox reaction transferases - Move group hydrolases - Cut with water lyases - Cut without using H2O or redox isomerases - Isomerize ligases - Combine |
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How does enzyme act as catalyst |
Stabalizing normal transition state or providing different pathway |
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Ways that transition states stabalize transitions states: |
Induced Fit Acid Base catalysis Covalent Catalysis Metal Ion catalysis |
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Cofactors of: Activation transfer vs Redox |
Activation transfer use the transfering group as cofactor Redox transfers electrons and does not from covalent bonds |
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Describe mechanism for serine Proteases |
Stabilizes intermediate through H bonding and covalent bonding. Holds Reactants where they need to be in order to be "attached" |
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Explain Michaelis-Menton Equation Kinetics |
Vi=(Vmax [S])/[Km+S] Initial speed = how fast it is 50% saturated Km= substrate affinity higher is greater affinity *also the substrate concentration needed to reach 1/2 Vmax Vmax= Max velocity at infinite concentration - Reaction won't go any faster |
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Compare Glucokinase vs Hexokinase with respect to their Km's |
Hexo: Low Km = fast uptake Gluco: Higher= slower uptake |
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How does Km and Vmax change with Comp vs noncomp inhibitors |
Competetive: Km higher Vmax the same Non Comp: Km SAME Vmax lower |
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How does enzyme concentration change with rates of transcription or degradation? |
More transcription= more enzymes
More degradation = less enzymes |
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Alosterism and activation vs inhibition |
Activation binds to keep it relaxed Inhibition binds to keep it tense |
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R vs T in enzyme bindong |
R=Relaxed and able to do work T= Tense and not able to work |
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Protenolysis |
Changing of Zymogens to heir active state by removing part of the protien |
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Reversale Phosphorilization |
Adding or removing a Phosphorylate group to activate to deactivate a protein |
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How does substrate concentration affect Vi? |
Higher S, higher Vi. Vmax times S and Km PLUS S |
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Thiamins phosporilase |
Cofactor |
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Biotin |
Water soluble B-Vitamin used as coenzyme |
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Pyrodoxal Phospate |
Coenzyme that reacts with amine group of amino acid |
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Covalent Modificaitons |
Phyosphoralization of Serine, Tyrosine and sometimes threonine Changes activity of protein Can be dephosphoralized and return to previous state. |
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Protien protien interactions |
When one protein interacts with another protein. GPCR for Ex.
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