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11 Cards in this Set
- Front
- Back
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how does affinity chromatography work?
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antigens are tagged on to the beads then the beads sort out the impure proteins
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what are the uses of affinity chromatography?
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atp binding proteins, drug binding proteins, metal binding proteins, and drug drug interactions.
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eptitope
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a small segment of a protein that binds to a particular antibody
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what is the fab domain?
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the part of an antibody that is specific for an antigen
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what is the fc domain?
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it is the part of the antibody that works with the immune response when an antigen binds to the antibody
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polycional antibodies
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a whole series of antibodies that is capable of binding to a antigen but specific parts of it
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polyclonal and monoclonal antibodies
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isolating specificity cells, cloning them, and then produces an antibody. this antibody can be used to detect various diseases such as AIDS, etc
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other ways to determine protein structure and function
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mass spect
protein sequencing 3d structure of protein biomed applic of synth peptides |
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protein sequences are most often deduced by
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DNA sequences
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NOESY
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Nuclear Overhauser Enhancement Spectroscopy. detects where the protons are located and from that one can determine the overall structure
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edman degredation
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the amino terminal is labeled and cleaved from the peptide without disrupting the peptide bonds between other animo acids and residues.
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