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18 Cards in this Set

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Diffusion Controlled RXN
A reaction that occurs with every collison between reactant molecules
Triose Phosphate Isomerase
catalyzes the rapid interconversion of DHAP and G3P in the glycolysis pathway
Superoxide Dismutase as a catalyst
Fast catalyst b/c of its negatively charged substrate is attracted to positively charged electric field near the active sit and because the reaction itself is very fast
Superoxide Dismutase
-Reacts wtih first thing it comes into contact with
-Does alot of damage
-Why Anti-Oxidants are so important
-Lou Gehring's less then normal Superoxide Dimutase
-concentration at any given time is very low, probability of 2 together is very low because they react slowly.
Superoxide Dismutase catalyzes the very rapid removal of ..........
the toxic superoxide radical anion, O2- a by product of oxidative metabolism, The enzyme catalyzes the conversion of superoxide to molecular oxygen and H2O2, which is rapidly removed by the subsequent action of enzymes suc as catalase.
Proximity Effect
The increase in the rate of a nonenzymatic or enzymatic reaction attributable to high effective concentrations of reactants, which result in more frequent formation of transitition states.
Proximity Effect- observed rates
The most restricted compound, rigid bicyclic has no rotational freedom so is therefore more likely to react because less entropy is lost during transition state.
Lysozyme
catalyzes the hydrolysis of polysaccharides that make up the cell walls of bacteria.
- in tears, saliva, and nasal mucus to prevent bacterial infection.
- In Egg white
Substrate of Lysozyme
Is a polysaccharide composed of alternating residues of N-acetyl-glucosamine and N-acetylmuramic acid connected glycosidic bonds
Stabilizes Lysozyme
4 disulfide bonds cross linked
Models of Lysozyme and it's complexes can be obtained by
X-Ray Crystallographic analysis.
Substate binding cleft of lysozyme accommodates
six saccharide residues at six sites.
Two ionic amino acid residues present
Glu-35 and Asp 52, are located close to C-1 of the distorted sugar molecule in the D binding site
Glu-35
is in the nonpolar region of the cleft and has a perturbed pka near 6.2
Asp-52
more polar enviroment, has a pka near 3.5
found in crevices
Optimum pH of lysozyme
5
pH affects Enzymatic Rates
The effect of pH on the reaction rate of an enzyme can suggest which ionizable amino acid residues are in its active site. Sensitivity to pH usually reflects an alteration in the ionizable state of one or more residues involved in catalysis and occasionally in substrate binding. Provided the enzyme in not denatured when the pH is altered, a plot of reaction velocity versus pH most often yields a bell-shaped curve.
Diffusion controlled rxn
A reaction that occurs with every collison between reactant molecules