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109 Cards in this Set
- Front
- Back
Carbohydrates:
How many kcal/g? What % of cal intake? Function? |
4 kcal/g
50-60% metabbed to glucose- fueel, assist in bowel elim. |
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Fats:
How many kcal/g? What % of cal intake? Function? |
9 kcal/g
30% Precursor for prostas and leukotrienes, carrier molecules for fat-soluble vitamins |
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Proteins:
How many kcal/g? What % of cal intake? Function? |
4
10-20% Source of the 9 essential AAs for synthing proteins and other N-containing substances. |
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What is the function of Vitamin A?
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Synthesis of rhodopsin (vision), cell differentiation and growth, antioxidant.
Fat soluble |
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What is the function of Vitamin B1 (thiamine)?
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Precursor for TPP, nerve conduction.
Thiamine, TT Deficiency- dermatitis, enteritis, alopecia, adrenal insufficiency |
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Which vitamin is a precursor for FAD and FMN?
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Vitamin B2 (riboflavin)
Flavin, FFF 2's a Fad |
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What is the function of Vitamin B3 (Niacin)?
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Precursor for NAD and NADP
Niacin, NNN you have 3 nads? |
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Which vitamin is a constiuent of Coenzyme A and Fatty Acid Synthase?
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Vitamin B5 (pantothenate)
Five Fatties named Amber Amber=Coenzyme A Deficiency is rarely seen, but causes dermatitis, hair loss, and GI upset. |
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What is the function of Vitamin B6 (pyridoxine)?
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Precursor for pyridoxal phosphate, heme synth
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Which vitamin is a cofactor in methionine metabolism and in propionyl coenzyme A?
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Vitamin B12- cobalamin
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What is the function of Vitamin C?
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Helps collagen synthesis, increases iron absorption in GI tract
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Which vitamin is responsible for increaseing CA absorption in kidney and GI?
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Vitamin D
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What is the function of Vitamin E?
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antioxidant
Deficiency- fragile erythrocytes., vision disturbances, neuro dysfunction, and myopathies. |
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What does Vit. K do?
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gamma-caroxylation of II, VII, IX, X.
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What is the function of Biotin?
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Cofactor for pyruvate carboxylase, propionyl coA carboxylase, and acetyl coenzym A carboxylase.
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What is the role of Folate?
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cofactor for 1-carbon transfers (methionine and nucleotide synth)
Blood, neuro |
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Which vitamins are Fat soluble?
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A, D, K, E
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Which vitamins are metabolic?
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B 1,2,3,5 and Biotin
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3 y/o boy with dark paatches of flaky skin adn pitting edema of his lower extremities, protuberant abdomen, muscle waisting.
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Kwashikor- The inadequate intake of protein with adequate calorie intake. Usually with weaning.
Marasmus- protein-calorie malnutrition from breast milk and cereal diet. Loss of weight, fat stores, and muscle mass. Protein mass is lost in heart, kidney, liver. Decreased resp function from muscle atrophy. Decreased CO. Decreased albumin-> edema. Immune malfuntion. Kworshikor- Skin, diarrhea, stunted growth, infection, pitting edema, hepatomegaly Marasmus- muscle wasting, infection, stunted growth, weakness, anemia |
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75 y/o with bad night vision. Constipation (oil laxative). 3 bouts of cold, scattered white patches on his conjunctiva as well as two poorly healed cuts on his left hand.
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Vitamin A (Retinol) deficiency
Etiology- fat malabsorption, oil laxative, malnutrition. 11-cis-retinol for rhodopsin Retinoic acid for cell growth and differentiation. B-carotene for antioxidant. |
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What happens in Vit A OD?
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arthralgia, fatigue, headache, skin changes, sore throat, alopecia, nausea, diarrhea, papilledema, hepatosplenomegaly.
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48 y/o with generalized weakness, increased pain on left side adn right thigh. Billroth II gastrectomy. Strength is 4/5. Pain on palpation of 5,6 ribs on left.
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Vitamin D deficiency
malabsorption, decreased sun 25- storage 1,25- active D3- sun D2- milk Rickets- kids, skeletal deformities., short, rachitic rosary, craniotabes Osteomalacia- diffuse bone pain in adults. muscle weakness. |
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What happens with Vit. D excess?
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Hypercalcemia, loss of appetite, stupor, Seen in sarcoid.
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72 y/o with ulcerative colitis, diabetes, recent foot ulcer, treated with abtics. multiple bruises, prolonged pt, ptt.
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Vit K deficiency
malabsorption and braod spectrum abtics, suppress bowel flora-> decreased K synth. Prolonged PT, PTT Normal bleeding time, thrombin time. Infants have a mild vit. k deficiency from poor diffusion of k across the placenta. |
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56y/o increased confusion. Inventing stories. Not drunk. Oriented to name, has nystagmus, decreased sensation to pinbrick from knees down. ataxic gate.
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Thiamine deficiency
Common in alcoholism. Precursor for TPP, conenzyme of carba nd AA metabolism. Nerve conduction. Beriberi and Wernicke-Korsakoff. Dry Ber1Ber1- polyneuritis, wasiting wet beriberi- high-output heart failure, edema Wernicke- encephalopathy- confusion, ataxia, and ophthalmoplegia Korsakoff- amnesia and confabulation |
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21y/o with weakness. eats 3-4 days per week. cracked skin at lips. vascularization of corneas.
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B2 deficiency
no Fad or Famn Dermatitis, glossitits, corneal vascularization, angular ceilitis |
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87 y/o with weakness. Watery, nonbloody diarrhea, difficult remembering. dark scaly patches on neck and back of hands.
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B3 (niacin) deficiency
Can come from Hartnup, isoniazid, carcinoid B3 is derived from tryptophan and B6. INH-decreased b6. Hartnup- decreased trp. Carcinoid- increased trp metab. Mild- weight loss, weakness, and glossitis Advanced- pellagra Pellagra's 3 D's Diarrhea, Dementia, Dermatitis. |
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What happens with excess Niacin?
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Reduce LDL and VLDL and increases HDL. SEs- Flushing, GI upset
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28 y/o with tonic-clinic seizure with no Hx of seizure disorder. On isoniazid. peripheral neuropathy. sideroblastic anemia.
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B6 deficiency
caused by INH, OCs Causes convulsions, hyperirritability, peripheral neuropathy. sideroblastic anemia precursor for pyridoxal phosphate. Carrier of Amine groups in transamination. Cofactor for cystathionine synth in methionine metab. In synth of heme. Excess can cause sensory neuropathy |
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24 y/o w/ onset of diarrhea, weakness, numbness in both legs. Social Hx- sushi. Impaired proprioception and vibratory sensation in both extremities. Smooth red tongue, ataxic gait. Labs- megaloblastic anemia, - schilling.
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B12 (Cobalamin) deficiency
Vegans, pernicious anemia, Crohn's, Diphyllobothrium latum, sprue, blind loop. Cofactor for homo to methionine, methylmalonyl coA to succinyl coA Neuro- ataxia, impared proprioception, vibratory sensation, glossitis, diarrhea. Megaloblastic anemia. |
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17 y/o male with generalized weakness, purpura, gingivaal swelling, blaeeding gums, and poorly healed cuts. Anemia.
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Vitamin C deficiency
dietary, elderly, alcoholics, homeless or pts with chronic illness hydroxylation of proline and lysine in synth of collagen. Facilitates Fe absorption. Scurvy- bleeding into joints, bleeing from gums, anemia, gigival swelling, impaired wound healing. |
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25 y/o with elevated chol levels. Muscle cramping, scaly skin, hair loss, chronic diarrhea.
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Biotin deficiency- abtic uses, raw egg whites (Avitin blocks absorption), long term parenteral nutrition.
cofactor for carboxylation: pyruvate->oxaloacetate Acetyl-CoA->malonyl-CoA Proprionlyl-CoA->methylmalonyl-CoA rare- dermatitis, Gi, muscle pain, elevated cholesterol. |
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32 y/o with new seizure disorder. taking phenytoin for 6 months. Tired with watery diarrhea. Pale, smooth red tongue, tachycardic. Megaloblastic
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Folate deficiency
Inadequate diet (alcoholics) Meds, sprue, MTX Pregs, hemolytic anemia Synth of dTMP Homo to meth |
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75 y/o with SOB, fatigue. Stool is tar-like, microcytic hypochromic anemia. What is causing the fatigue?
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Fe deficiency
Blood Loss, diet Decreased serum Fe, ferritin, increased transferrin saturation. |
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What happens with too much Fe?
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increased free radicals. oxidation of LDL, CV disease.
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63 y/o with chronic renal insufficiency. Wrist spasms, increased pain in lumbar spine. Decreased urine output.
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Calcium deficiency
malnutrition, renal failure, endocrine Muscle cramps, tetany, prolonged QT, + Trousseau, Chvostek, Bone pain Trousseau- spasm with bp cuff removal Chvostek- twitching of facial muscle with tapping of nerve. |
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What happens in Iodine deficiency?
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needed for T4
hypothyroid, cretinism, myxedema, goiter |
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What happens in Mg deficiency?
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Binds to ATP
Deficiency- increased excitibility, tetany, vent arrhythmias, hypocalcemia |
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What happens with P deficiency?
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Phosphorus- constiuent of bone, cell, nucleic acid
deficiency- rare, but bone pain, skeletal malformations, anemia, platelet malfunction, encephalopathy |
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What happens in Zinc deficiency?
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Zn- cofactor for Metalloenzymes
Dermatitis, increased infection, stunted growth, altered mental status. |
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How is EtOH metabbed?
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Alcohol dehydrogenase and acetaldehyd dehydrogenase break it down.
NAD is the limiting reagent Zero order kinetics for alcohol dehydrog Disulfiram ihibits acetaldehyde dehydrogenase (acetaldehyde=hangover) |
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What happens in EtOH hypoglycemia
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Increased NADH/NAD ration in liver.
Pyruvate to lactate OAA to malate Inhibbed gluconeo, and hypoglycemia. Fatty change in liver |
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What are some DNA excision repair defects?
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Xeroderma pigmentosa- UV- thymidine dimers
Ataxia-telangiectasia- X-ray Blooms- radiation Fanconi- cross-linking agents |
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How are DNA, RNA and protein synthed?
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5->3
5' has the energy AA's are linked N-C |
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What are the types of RNA?
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mRNA- messenger- largest- transcription
rRNA- ribosomal- most abundant- protein synth tRNA- transfer- smallest translation |
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What is restriction fragment lenght polymorphysm analysis (RFLP)?
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It is the DNA fingerprint.
Everyone has polymorphisms. These get clipped into fragments. These fragments are different in everyone. |
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What is PCR?
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A process of making multiple copies of a single portion of DNA.
Denatured to generate 2 strands. Cooling, excess primers annel to a site to be amplified heat-stable DNA polymerase replicates the sequence. |
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What is ELISA?
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Ag-Ab
two ways to test: 1) Ag to see if body recognizes it 2) Ab to see if body has Ag. |
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What are the roles of the following:
RNA polymerase I RNA polymerase II RNA polymerase III TATA and CAAT sequences 7-methylguanine Poly A polymerase AUG sequence UGA, UAA, UAG |
I- rRNA
II- mRNA III- tRNA TATA- promoter sequence where mRNA binds. 7-methylguanine- caps the mRNA to protect from degradation. Poly A- creates a tail to stabalize the mRNA AUG- start codon- met in eukaryotes, f-met in prokaryotes UGA, UAA, UAG- stop codons |
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What is a enhancer? an operator?
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Enhancer- binds Transcription Factors
Operator- binds repressors. |
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Exons and Introns
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EXons are EXpressed.
snRNP splice out Introns. Exons are ligated together |
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What is unmodified mRNA called?
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hnRNA
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tRNA:
structure wobble theory function |
CCA at 3' binds AA
Center loop is the anti-codon Wobble theory- 3rd bp is different, but same AA needed to deliver AAs in the appropriate sequence. |
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What is the role of aminoacyl-tRNA synthetases
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Attaches AA to the tRNA
Uses 1 ATP mischarged tRNA leads to mismatch |
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what is a Ribosome? just givve some structural details.
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A site: binds tRNA containing the next AA to be added to the peptide
P site: binds tRNA containing growing peptide chain. |
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What is the role of peptidly transferase?
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Peptidly transferase- enzymatic component of the large ribosomal subunit. catalyzes A-site AA.
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What is the pattern of mitochondrial inheritance?
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Transmitted by mom, all kids will be affected.
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Define Variable Expression
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Same genotype can have different phenotypes
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Define Incomplete Penetrance
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Not all those with a genotype will have the phenotype. Penetrance is the likelihood that the phenotype will develop.
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Define pleiotropy
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1 gene has multiple effects on multiple organs
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Define Imprinting
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phenotype is different based on whether it is maternal or paternal.
AngleMan- Mom Prader-Willi- Pop |
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Define Anticipation
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Disease worsens in each generation due to increased repeats. Huntingtons, Fragile X
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Define loss of heterozygosity
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Pt inherits or develops a mutation in a tumor suppresor gene, the complement must be deleted before cancer develops. (not true of oncogenes)
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Define dominant negative
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dominant effect. altered gene blocks good gene in a heterozygote
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Define Linkage disequilibrium
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groups of alleles are inherited together. (by population)
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Define Mosaicism
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cells have different genetic make-up
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Define Locus heterogeneity
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Mutations at different loci have the same phenotype.
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Define Codominance
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Two alleles for the same gene in a heterozygote are both expressed, AB blood.
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Define incomplete dominance
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Two alleles of the same gene give a mixed phenotype.
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What are the conditions of the Hardy Weinberg population?
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Large population
Random mating no emigration or immigration no mutations no incest |
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what are the equations of hardy-weinberg?
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allele prevalence- p+q=1
disease prevalence- p2+2pq+q2=1 |
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If sickle cell is an autosomal recessive disease that occurs in 1 out of 400 births, what is the frequency of carriers of the sickle cell trait?
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q2=1/400
q=1/20 p=1-q=19/20 2pq=(1/20)(19/20)2=.095 95/1000 is a carrier |
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Fragile X is an X-linked disease that occurs in 1 out of every 2000 males. what is the frequency of female carriers?
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q=1/2000
p=1-q=1999/2000 2pq=2(1/2000)(1999/2000)=.0009995 1/1000 |
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What are the autosomal trisomies?
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Downs Syndrome (21) (D- Drinking age is 21)
Edwards' Syndrome (18) (E- Election age 18) Patau's (13) (P- Puberty 13) |
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46 y/o mom. baby girl born with flat face, wide-set eyes, epicanthal folds, and 1 palmar crease across each hand. Holocystolic murmur with ventricular septal defect.
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Down's syndrome- Trisomy 21
95% meiotic nondisjunction. 4% Robertsonian 1% mosaicism Description as in case endocardial cushion defects are common. Increased risk for ALL and Alzheimers. Increased age is bad for moms. Decreased alpha-fetalprotein, Beta-HCG, Increased nuchal translucency. |
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What is Edwards Syndrome?
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Trisomy 18
Mental retard, rocker bottom feet, low-set ears, micrognathia, heart disease, clenched hands, prominent occiput. Death in 1 year. |
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What is Patau's syndrome?
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Trisomy 13
severe mental retardation, microphthalmia, microcephaly, cleft lip/palate, abnl forebrane structures, polydactyly, heart disease, death in 1 year. |
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What is cri-du-chat syndrome?
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deletion of the short arm of 5.
microcephaly, mental retard, high-pitched mewing, epicanthal folds, cardiac abnlties. |
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What is the Catch-22 of 22q11?
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Cleft Palate
Abnormal facies Thymic aplasia-> bad T-cells Cardiac defects Hypocalcemia 2ry to parathyroid aplasia. velocardiofacial syndrome CAC DiGeorge TCH |
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What is the difference between Angelman and Prader-Willi Syndrome?
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AngelMan- Mom
Prader-Willi- Pop Both are a 15q deletion. PW- retard, hypogonad, obesity Angelman- Happy Puppet with ataxic gait, innappropriate laughter, retard, seizures |
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What causes Klinefelters?
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Maternal meiotic nondisjunction- 47XXY
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2 week old girl with possible congeinal heart defect. weak femoral pulses and broad chest with widely spread nipples. cystic hygroma on the neck (webbed neck)
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Turner's Syndrome
Monosomy X Coartaction of the aorta webbed-neck broad chest short |
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What causes Fragile X?
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CGG repeats in the familial mental retardation gene on the X.
Large testes, large jaw, ears. CT defect with hyperextensible joints and MVP. |
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What disorders have trinucleotide repeats?
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Try hunting for my Fried eggs (X)
Hungtingtons myotonic dystrophy Friedreich's ataxia Fragile X |
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What are some common congenital malformations?
seven |
1. Heart defects
2. Hypospadias 3. Cleft lip 4. congenital hip dislocation 5. spina bifida 6. anencephaly 7. Pyloric stenosis. |
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Enzyme kinetics, what is the relationship between Km, Vmax and S?
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the lower the Km, higher the affinity.
Km=[s] at 1/2Vmax competative inhibitors cross. |
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What are the differences between competative and non-competative inhibitors?
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Competative inhibitors resemble substrate
Overcome by an increase in [s] Bind active site. Km increases no change in Vmax Vmax decreases with noncompetitive inhibitors |
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How are enzymes regulated?
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alteration synth/destruction
covalent modification- Phosphorylation proteolytic modification- zymogen allosteric regulation- feedback inhibition transcriptional regulation- steroid hormones |
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Rough endoplasmic reticulum:
what does it do? |
Synthesis of secretory proteins adn N-linked oligosaccharide addition
Goblet cells and Plasma cells have mad RER |
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What are Nissl bodies?
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Rogh ER, in neurons; not found in axon or axon hillock.
Synthesize enzymes- ChAT adn peptide neurotransmitters |
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What does the smooth endoplasmic reticulum do?
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Steroid synth and detoxification of drugs and poisons.
Liver hepatocytes and steroid hormone- produce cells of the adrenal cortex. Rich in SER. |
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What are the 6 functions of the golgi apparatus?
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1. Distribution center of proteins and lipids from ER to the plasma membrane, lysosomes, and secretory vesicles.
2. Modifies N-oligosaccharides on asparagine 3. Adds O-oligosaccharides to serine and threonine residues 4. Proteoglycan assembly from proteoglycan core proteins 5. sulfation of sugars in proteoglycans and of tyr on proteins. 6. mannose-6-phosphate to specific lysosomal proteins, whcih targets the protein to the lysosome. I-cell disease- lacks mannose-6-phosphate DAT Poor Small Man Distribution Asparagine Threonine Proteoglycans Sulfation Mannose |
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Microtubles
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Alpha and beta tubulin dimers. Each has 2 GTP bound. Incorporated into flagella, cilia, mitotic spindles.
Grows slowly, collapses quickly. Slow axoplasmic transport in neurons. |
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What drugs affect microtubules?
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Mebendazole/thiabendazole (antihelminth)
Taxol (breast cancer) Griseofulvin (antifungal) Vincristine/vinblastine (anticancer) Colchicine (anti-gout) |
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What is Chediak-Higashi syndrome
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microtubule polymerization defect resulting in decreased phagocytosis.
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How is cilia structured?
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9+2 microtubule arrangement.
Dynein is an ATPase that links peripheral doublets, bending of cilium. |
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What is Kartagener's syndrome?
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Dynein arm defect- immotile cilia.
Dynein=retrograde kinesin=anterograde |
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What is the composition of the plasma membrane?
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Cholesterol 50%- stability
Phospholipids 50% shingolipids, glycolipids and proteins. Hich chol or long saturated fatty acid contennt increases the melting temperature. Noncytoplasmic side contains glycosylated lipids or proteins. |
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What is the function of Phosphatidly choline?
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Lecithin, major component of RBC membranes, of myeline, of bile and of surfactant. DPPC- dipalmitoyl phosphatidylcholine) Also in the esterfication of cholesterol (LCAT is lecithin-cholesterol acyltransferase)
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What's the big deal with Na-K ATPase pump?
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plasma membrane. 3 Na out, 2 K in. pump is phosphorylated during cycle.
Ouabain inhibits by binding to K site. Digoxin, also inhibit causeing increased contractility. |
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What receptors use the phospholipase C pathway?
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HAVe 1 M&M Gq
H1, alpha1, V1, M1, M3 Phospho C-> PIP2 -> IP3 or DAG IP3->Ca2+ DAG->Protein Kinase C |
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What receptors are Gs?
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Excitatory on Adenylyl cyclase.
B1, B2, D1, H2, V2 Increases cAMP |
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What receptors are Gi?
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Inhibitory of Adenylyl cyclase
MAD2s M2, A2, D2 |
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What are the different types of Collagen?
|
Be Cool, Read Books
I- Bone, tendon, skin, dentin, fascia, cornea, late wound repair II- Cartilage, vitreous body, nucleus puposus III- Reticulin- skin, blood vessels, uterus, fetal tissue, granulation IV- Basment membrane X- epiphyseal plate. |
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How is collagen synthed?
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Inside Fibroblasts
1. Alpha chains (Preprocollagen) made on RER 2. ER- hydroxylation of proline and lysine residues (vit C) 3. Golgi- glycosylation of Pro-alpha-chain lysine residues and formation of procollagen. 4. procollagen are exocytosed Outside fibroblasts 5. Procollagen peptidases cleave. tropocollagen 6. staggered tropos covalent lysine-hydroxylysine make collagen fibrils. |
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What is the defect in Ehlers-Danlos?
|
defect in collagen cross-linking
Hyperextesible skin bleed easily hypermobile joints |
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What is the defect in Osteogenesis imperfecta?
|
AD disorder in formation of procollagen
1. Multiple fractures 2. Blue sclerae from translucency of the connective tissue over the choroid 3. Hearing loss (bad middle ear) 4. Dental imperfection |
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What types of cells do the following stain:
Vimentin Desmin Cytokeratin Glial fibrillary acid proteins Neurofilaments |
Vimentin- CT
DesMin- Muscle CytoKERATIN- Epithelial Glial FAP- NeuroGLIA Neurofilaments- Neurons |
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What is metabolized in the mitochondria, cytoplasm, both?
|
Mitochondria- Fatty Acid ox, Acetyl-CoA production, Krebs
Cytoplasm- Glycolysis, Fatty synth, HMP shunt, Protein synth, steroid synth Both- Gluconeogenesis, urea cycle, heme synth |