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15 Cards in this Set

  • Front
  • Back

Sterilization

Removal and destruction of all microbes

Disinfection

The destruction of all vegetative microbes

Contamination

Refers to the presence of microbes

Contaminant

A substance that:


A) Does not belong period


B) Does not belong at certain levels

Decontamination

Removal of contaminants to any degree

Antiseptic

Pathostatic agent used on a biological level to prevent damage

Microbial death

The permanent loss of reproductive capability even under optimal growth conditions

Optimal growth conditions

Nutrients, atmosphere, temperature correct

Factors which affect microbial death rate

Microbe count


Type of microbe


Environment temperature and conditions


Concentration of agent


Type of agent


Presence of solvents, organic matter, or inhibitors

Low resistance microbes

Fungal spores


Yeasts


Enveloped viruses


Protozoan trophozoites (vegetative bacteria)

Moderate resistance microbes

Protozoan cysts


Fungal sexual spores


Naked viruses


Some vegetative bacterial cells: E.g. Tuberculosis, S. aureus, pseudomonas

High resistance microbes

Prions


Endospores

Thermal death time (TDT)

Shortest time require to kill a population of microorganisms at a specific temperature

Thermal death point

Lowest temperature required to a kill a population of microorganisms in 10 minutes

Most common autoclave input

12psi bar, 121C, 15-20 minutes