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4 Cards in this Set
- Front
- Back
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What is in a Polymerase Chain Reaction single tube?
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Template DNA, DNA polymerase, dNTP's, Buffer, A pair of single-stranded Oligonucleotide Primers that hybridize to a specific genomic position
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The PCR is run for many cycles; each cycle has three steps. What are they?
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Heat Denature 95 C; Anneal Primers 60 C; Polymerization (Taq pol + dNTP's) 72 C
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Sanger “dideoxy” sequencing
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DO WE NEED TO KNOW THIS?
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"Dye-Terminator" Sanger sequencing
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DO WE NEED TO KNOW THIS?
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