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8 Cards in this Set
- Front
- Back
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What are restriction enzymes?
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(restriction endonucleuses) These are special enzymes that bind to short sequences on the DNA.
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What do restriction enzymes look like?
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They are symmetrical and have the same nucleotide sequence, but run in opposite directions. EX: GAATTC=CTTAAG
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What is genetic engineering?
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Moving genes from one organism to another.
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What are the 4 stages that gene transfers share?
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(CPCS) Cleaving, Producing Recombinant DNA, Cloning, and Screening
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Describe cleaing DNA
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Cutting the source and vector DNA, it separates different groups of DNA fragments by electrophoresis and restriction enzymes.
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Describe the production of recombinant DNA
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Recombinant DNA is formed to enter the host cell; it is made from parts of source DNA and parts of bacteria or plasmids.
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Describe cloning
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Infecting target cells with DNA-bearing vectors and allowing infected cells to reproduce. When reproduced, they form a clone-library.
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Describe screening DNA
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Selecting the particular infected cells that have reveived the gene of interest. They do this by using specific vectors that attract the gene of interest.
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