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10 Cards in this Set

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  • Back
restriction enzymes
cut DNA molecules in specific places.
vector molecule
each cut-up fragment becomes a vector. A carrier capable of transporting the DNA fragment into a cell.
2 types of vectors
bacteriophages, plasmid
plasmid
smaller, circular dna molecule
transformation
uptake of foreign dna by cells
recombinant plasmid
foreign dna spliced into it.
sticky ends
result of cutting dna in a staggered fashion. b/c they pair (by HB) w/ complementary, single-stranded ends of other fragments.
2 sticky ends atttach
treated with DNA ligase- links the ends to form a recombinant dna
genome
total DNA per cell
genomic library
collection of thousands of dna fragments that represents all the dna in the genome.