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10 Cards in this Set

  • Front
  • Back
restriction enzymes
cut DNA molecules in specific places.
vector molecule
each cut-up fragment becomes a vector. A carrier capable of transporting the DNA fragment into a cell.
2 types of vectors
bacteriophages, plasmid
smaller, circular dna molecule
uptake of foreign dna by cells
recombinant plasmid
foreign dna spliced into it.
sticky ends
result of cutting dna in a staggered fashion. b/c they pair (by HB) w/ complementary, single-stranded ends of other fragments.
2 sticky ends atttach
treated with DNA ligase- links the ends to form a recombinant dna
total DNA per cell
genomic library
collection of thousands of dna fragments that represents all the dna in the genome.