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39 Cards in this Set

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Who made the first microscope


The Janssen brothers Hans and Zacharias Janssen

Brothers

Who made the second microscope


Robert hooke his micro scope was a compound microscope with a third lens and a light

Who made the third microscope

Antoni van leeuwenhoek his was a simple microscope using one lens which was able to magnify onjects up to 250x and was the first to view single celled organisms

What is magnification

How many time larger the image is than the original

What is the power of the eye piece lens and the objective lenses

Eye piece lens: 10x


Low power:4x


Medium: 10x


High: 40x


What is field of view

The area that can be seen through the microscope with a certain objective lens

What happens when lens power in creases

Field of view decreases

What is scale

A comparison between orignal size and the smaller scale

What did gallileo propose

He proposed that the earth revolved around the sun, but the church did not accept his theory becaue the church belived that the earth was the center of the universe. So he was put under house arrest and later burned alive at the steak but received a papal pardon 350 years after his death by pope john paul II

What is spontaneous generation

Living things can emerge from non living objects because of the life force that it contained

Who was the first person to try and disprove spontaneous generation

Francisco redi by putting meat in two different containers one with access to air and one without but his experiment led people to belive that spontaneous generation needed air

Who was the first person who tried to prove spontaneous generation

John needham he boiled chicken broth and sealed it in a flask and still found that microorganisms still appeared ( because the broth was not heated for a long enough time or at a high enough temperature)

Lazzaro spallanzani did what

He tried to disprove spontaneous generation by removing all air from a container by using a vacume but this only proved that air was needed

Who disproved spontaneous generation

Louis pasteur he disprove d spontaneous generation

Who was robert brown

He is the person who discovered the nucleus being responsible for control ing the cell functions

What did Schleiden and schwann propose

They proposed all plant and animals are composed of cells and that they are the basic unit of life

Who was rudolf virchow

He is the person who discovered that cells come from preexisting cells

What is the cell theory

All living thing are made up of 1 or more cells



They are the smallest unit of life



Cells are produced from preexisting cell through cell division called mitosis

What is contrast

Variations in light and shadow

How has image quality improved

New staining techniques and new methods of illumination

What is resolution

The ability to distinguish between two structures that are close together

How much is the human eye capable of resolving

Up to 0.1mm or larger

What is fluorescent microscopy

Fluorescent stains called GFP (green fluorescent protein)



Different cell structure s absorve stains in different amounts



Depending on the type of dye the cell glows either yellow, orange or green



GFP does not kill the cell

What does a brightfield microscope do

A light passes through the specimen and staining the specimen kills it and you cant view living tissues. And you cant get a higher resolution than 0.2 um

Electron microscope

A fine beam of Electron are shot at the specimen the electrons pass through depeneding on density. And use magnetic fields to focus image

Transmission electron microscope ( tem)

100x better magnification and resolution than a light microscope


It is difficult to produce 3D images


Living specimen s cannot be observed

Scanning electron microscope ( sem)

The specimen is fixed and coated in gold. Electrons reflect off of gold and prduce 3D images 2x better quality than the TEM and new SEMs permit use of live materials

Gene mapping

Decoding species genes

What can gene mapping help us with

Help us understand where diseases come from and how to treat them effectively



Also allows us to create new varieties of plants

What does GMO stand for

Genetically Modified Organisms

Are cell open or closed systems

They are open systems they must interact with the environment to survive

What are hormones

Are chemicals produced in one part of your body that acts on a different part

What is an example of a hormone created in your brain and when is it created

Adrenaline is produce when excited or stresed

What are receptors

Transmitters to attach to the cell and cary out their function

Only transmitters with the correct shape can

Dock at a certain receptor it is like a lock and key

Viruses and bacteria can trick cells by

Mimicking the shape of a harmless molecule

You immune system fights off invaders by

Identifying them based on the markers on their cell membranes

Cell communication helps


Scientist

Diagnose viruses and bacteria


-diseases in the immune system


-make more effective medications

Parts of a microscope

-eye piece


-body tube


- course adjustment knob


-fine adjustment knob


-arm


-base


-light source


-diaphragm


-stage


-stage clips


-objective lens


-revolving nosepiece