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9 Cards in this Set

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Give three examples of post-transcriptional control of gene expression.
1)Affect mRNA stability 2)Binding of translational inhibitory proteins to UTR of mRNA.
3)Binding of micro-RNAs to UTR of mRNA
What are two examples of post-translational regulation of protein activity?
1)Phosphorylation.
2)GTP loading.
What molecular techniques are used to assess differential gene expression/activity?
1)Northern blot 2)Western blot 3)Microarray
What techniques are used to obtain spatial information about gene expression?
1)In situ hybridization 2)Immunocytochemistry
What techniques allow you to assess the function of regulatory DNA elements?
1.Reporter assay 2.Enhancer trap 3.Promoter bashing
What is the receptor in the Wnt/beta-catenin pathway?
Frizzled
What are the Co-receptors in the Wnt/beta-catenin pathway?
1.Lrp - LDL-receptor related protein
2.Glypicans – sulfated proteoglycans
What are the Intracellular factors in the Wnt/beta-catenin pathway?
1)APC/Axin/GSK-3 2)Dishevelled 3)Beta-catenin
What experiment gave evidence that a gradient is required for pattern formation?
Klaus Sander tied strings around drosophila embryos. No middle segments formed (anterior and posterior segments developed normally)