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67 Cards in this Set

  • Front
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Prokaryote bio

Evolved approximately 3.5 bil years ago & were the only life forms on the planet for approx 1.5 billion years




Most abundant organism on the planet found in almost all habitats




Approx.5K species have been named w/ an estimated 1.5 billion species believed toexist.

Prokaryote Structure

Nomembrane bound organelles.




Have a single circular chromosome called a ‘geosphere’ located in the ‘nucleoid’region of the cell.




Many Cells also have smaller pieces of ring-likeextrachromosomal DNA called ‘plasmids’ that can give these additionalcharacteristics

Extra bacteria features

capsule - gelatinous coating aiding inadhesion to substrates




endospore - tiny, thick-walled, identical dormant cell, resistant to unfavorable conditions.




Pili/Pilus - long filamentous extensions to adhere a cell to its substrate and conjugation in somespecies.

3common cell shapes

bacillus - rod/caplet




coccus - spherical




spirillum - helically coiled

2common colonial arrangements

Staph - grape like




Strep - chain like

Gram stain results

G+ = purple




G- = pink

Gram stainingprocedure

Primary - c. violet - stains whole sample




Mordant - iodine - binds stain to PGL




Decolorize - ethanol - lipid solvent and protein desiccant




Counter stain - safranin - stains any newly exposed PGL pink

Prokaryotes Motility

1-Gliding on slimy strands




2-twisting of helically coiled cells




3-flagella

What is different in Prokaryote's flagella?

-No ‘9+2’ arrangement




-consists of a solid core of a protein called flagellin.




-used in a propeller fashion

Taxis

Movement toward (positive) or away from (negative) a stimulus

Prokaryotes Reproduction

asexually by binary fission .

Prokaryotic Sourcesof Diversity

Mutation - ultimate sources




Transformation - ability of prokaryote cell to absorb & express extracellular DNA.




Transduction - genetic diversity conferred by a viral infection.




Conjugation - Genetic diversity conferred by the use of a pili.

Prokaryotes NutritionMode

1)Photoautotrophs - use of light energy to perform carbon fixation (CF)




2) Chemautotrophs - use of energy from oxidation of inorganic molecules to perform CF


3) Photoheretrophs - use light energy ATP but cannot perfrom CF.




4) Chemoheterotroph - cannot use light energy or CF.

Nitrogen Fixation

ability to convert molecular Nitrogen (N2) into a nitrogenous compound such as nitrite (No2-),nitrate (NO3-), ammonia (NH3)

MetabolicResponse to O2

Obligate Aerobes – must have O2 tosurvive



Facultative Aerobes – will use O2 if present but in its absence are capable of switching over to fermentation




Obligate anaerobe – O2 is lethal

Ecological Importanceof Prokaryotes

Decomposers




Commercial Production




Bioremediation – use of microbes to clean up waste




Symbionts



Domain Archaea Taxonomy

Prokaryotes that do not have PGL in their cell wall.


Overall majority or extremophiles = thrive in environmental extremes.




1.Group Euryarchaeota


2.Group Crenarchaeota


3.Group Korarchaeta


4.Group Nanoarchaeota

Group Euryarchaeota

Domain Archaea




●Includes the methanogens (swamps, GI), extreme halophiles (salty) and some thermophiles(extreme heat).

Group Crenarchaeota

Domain Archaea




●Includethe majority of thermophiles

Group Korarchaeta

Domain Archaea




● Recently discovered thermophile archeans.





Group Nanoarchaeota

Domain Archaea




● Archeans less than .5 micrometer in diameter.





Domain Bacteria Taxonomy

1.GroupProteobacteria




2. GroupChlamydias




3. GroupSpirochaetes




4. GroupCyanobacteria




5. Group Gram Positive Bacteria

Group Proteobacteria

Domain Bacteria



Includes the ancestors to eurkaryotic mitochondria, nitrogen-fixing bacteria like 'Rhizobium',and pathogens that cause salmonella, cholera, and Rocky Mountain spotted fever.


Group Chlamydias

Domain Bacteria




Existo nly as endosymbionts




Many species are zoonotic




Includes the organism responsible for the STD Chlamydia as well as those causing respiratory and eye infections.

Group Spirochaetes

Domain Bacteria




● All members have a helically-coiled cells.


● Includes organisms responsible for lyme disease and syphilis.

Group Cyanobacteria

Domain Bacteria




aka Blue-Green algae (very slimy unlike true algae).




ancestor of Eukaryotic chloroplasts




also use phycoerythrin/phycocyanin









Group Gram Positive Bacteria

Domain Bacteria




● all members test positive with a gram stain.




●Includes the organism responsible for the small of freshly-plowed earth.




● Also bacteria causing: Anthrax, botulism, TB,& leprosy

KingdomProtista history

Extremely diverse Kingdom, containinganimal-like, plant-like, and fungal-like representatives.


evolved approx. 2.0 billionyears ago and were the only eukaryotic organisms on the planet for at least 1.5billion years.




Believed to have Originated(at least partly)through endosymbiosis in which larger prokaryotes phagocytized smallerprokaryotes

Kingdom Protista structure/ enviroment



Maybe unicellular, colonial, or even simple multicellular sheets.




Overallmajority are aquatic or restricted to moist environments.

Kingdom Protista Reproduction

asexually by mitosis (a few by binary fission)



and sexually by meiosis.

Kingdom Protista nutrition mode

Maybe photoautotrophic, chemoheterotrophic or both “mixotrohphic”

Kingdom Protista locomotion

Pseudopodia(false feet) – cytoplasmic extensions used for locomotion and feeding.




Cilia– shorter and typically more numerous than flagella; used like boat oars




Flagella– longer and less numerous; used in whip like fashion in “9+2” microtubule arrangement.

Protist taxonomy

1. SupergroupExcavata




2. SupergroupChromalveolata




3. SupergroupRhizopia




4. SupergroupArchaeplastida




5. Supergroup Unikonta

1. SupergroupExcavata

Many members have an elongated or “excavated” groove on one side of the cell and isused for feeding.




-Group Diplomonadia (diplomonads)


-Group Parabasala(trichomonads)


-Group Euglenozoa (euglenoids)

Group Diplomonadia

Supergroup Excavata




aka (diplomonads)




Havemodified mitochondria (mitosomes) that lack an ETS so are home anaerobic environments.




Ex.Giardia lamblia – causes “giardiasis” or“backpackers diarrhea”

Group Parabasala

Supergroup Excavata




aka trichomonads




Havemodified mitochondria called ”Hydrogenosomes” that lack an ETS




Cellstypically use flagella together with an undulating membrane for locomotion.




Ex.Trichomonas vaginalis – causes STD “trichomoniasis”

Group Euglenozoa

Supergroup Excavata


aka euglenoids


Inaddition to the 9+2 microtubule arrangement, their flagella also have aspiral/crystalline rod.



-Subgroup Euglenophyta


-Subgroup Kinetoplastida

Subgroup Euglenophyta

Supergroup Excavata


Group Euglenozoa




Unicellular, Mixotrophic




Cellhas an anterior pocket from which one or two flagella emerge




Hasa red “eye spot” capable of distinguishing dark form light.




Ex.Euglena

Subgroup Kinetoplastida

Supergroup Excavata


Group Euglenozoa




Cellshave a “kinetoplast”- a single large mitochondria with an organized massof DNA




Maybefree living or parasitic




Ex.Trypanosoma – causes “sleeping sickness”

SupergroupChromalveolata

Believedto have evolved over 1 billion years ago through secondary endosymbiosis inwhich an ancestor protest phagocytized a red algae.




Grouop Alveolata (3 sub)




Group Stramenopila (4 sub)





Group Alveolata

Supergroup Chromalveolata




Cellshave tiny membranous sacs called alveoli just under the surface of theplasma membrane.




SubgroupDinoflagellida


Subgroup Ciliata


Subgroup Apicomplexa

SubgroupDinoflagellida

Supergroup Chromalveolata


Group Alveolata


aka dinoflagellates




Unicellular/colonial, Mixotrophic




Cells are bi-flagellate w/ the flagella arranged perpendicular to each other and are recessed in flagellar grooves.




Cellshave a species specific shape reinforced by internal plates of cellulose




reproduce on a massive scale resulting as a bloom (red/brown)




Theyare “bioluminescent”




Some called “zooxanthellae”, form mutualistic symbiotic w/ cnidarians (corals) - gives corals brightercolors (pigments)




Ex. Ceratium

Subgroup Ciliata

Supergroup Chromalveolata


Group Alveolata


aka Ciliates


unicellular , free-living, chemoheterotrophs




Usecilia for locomotion. cilia may be localized or cover entire body




Macronucleus(1)- controls everyday activities and asexual reproduction



Micronuclei(1-80) – necessary for sexual reproduction.




Ex:Paramecium/Vorticella

Subgroup Apicomplexa

Supergroup Chromalveolata


Group Alveolata


aka Sporozoans




Noknown means of locomotion




Allare parasitic and exist internally in hosts.



Havecomplex organelles at the apex of the cell that contains hydrolytic enzymes fordigesting host tissues.




Ex. Plasmodium vivax - malaria

Group Stramenopila

Supergroup Chromalveolata




Cellsoften have a prominent hairy flagellum together w/ a shorter smooth flagellum.

SubgroupBacillariophyta

Supergroup Chromalveolata


Group Stramenopila


aka diatoms




Maybe unicellular or colonial




Cell walls composed of silica and consist of 2 halves




Allare photosynthetic and produce an estimated 40-80% of Earth’s oxygen




Storeexcess energy as oil for buoyancy




use infiltration systems, as polishes, and in toothpaste.

Subgroup Chrysophyta

Supergroup Chromalveolata


Group Stramenopila


aka golden algae




-Freshwater and marine


-Cellsare bi-flagellate


-Unicellular/colonial, Mixothrophic


-Gettheir color from pigment Xanthophyll ----Capableof ‘blooms’ when conditions are right.

Subgroup Phaeophyta

Supergroup Chromalveolata


Group Stramenopila


aka kelps




-multicellular, primarily marine


-Gettheir color from the pigment fucoxanthin-largest of the algae, grow to over 120 ft in length


-features analogous to true plantso Blade, Stipe, Holdfast


-Some produce pneumatocysts (air bladder) for buoyancy

Subgroup Oomycota

Supergroup Chromalveolata


Group Stramenopila


aka egg fungi/ water mold




Superficiallyresemble true fungi “hyphae” and “mycelium”.




Thehyphae/mycelia increase surface area to support “saprotrophic”nutrition mode.




Ex. Saprolegnia

SupergroupRhizopia

Amoeboidprotists w/ thread like pseudopodia.




Group Foraminifera


Group Radiolaria

Group Foraminifera

SupergroupRhizopia


akaforams




Havemulti-chambered, porous, (CaCo3) shells.




Reticulopodia areextended through the pores for feeding and locomotion




overall majority are sessile (attached to a substrate)

Group Radiolaria

SupergroupRhizopia


aka heliozoans /radiolazians




Unicellular




Havean endoskeleton composed of silica




Have axopodia (long thin pseudopodia) radiatingoutward in all directions for feeding and buoyancy.

SupergroupArchaeplastida

Evolved over 1billion years ago through primary endosymbiosis in which an ancestor protestphagocytized a cyanobacterium




Group Chlorophyta


GroupCharophyta


GroupRhodophyta



Group Chlorophyta

SupergroupArchaeplastida


aka green algae




Unicellular,colonial or simple multicellular sheets




Getcolor pigment chlorophyll




form mutualistic symbiotic w/ ascomycete (sac) fungiresulting in a lichen




Ex. Spirogyra, Volvox, Ulva

GroupCharophyta

SupergroupArchaeplastida


aka stone warts




-believed to have given rise to kingdom plantae


-both charophytes & true plants have:


*cellulose cell walls,


*both chlorophyll a & b,


*similar peroxisome & flagellated spermstructure


*heterogamous/anisogamous gametes


*form a phragmoplast (cell plate) duringcytokinesis

GroupRhodophyta

SupergroupArchaeplastida


aka red algae




-Get their color from the pigment phycorythrin


-All are multicellular andprimarily marine like brown algae but smaller


-the deepest growing algae with some surviving at depths over 600 ft deep.


-Some members incorporate (CaCO3) into their cell walls, giving them a stoney appearance and are commonlycalled coralline algae

Supergroup Unikonta

Includes unikont (single flagellated) protists and/or those thatevolved from unikont ancestors.




Group Amoebozoa (4 subs)




GroupOpisthokonta (2subs)

Group Amoebozoa

Supergroup Unikonta

SubgroupGymnamoeba

Supergroup Unikonta


Group Amoebozoa




Free living amoebid protists living in freshwater andmarine aquatic enviroments or most soil




Ex. Amoeba proteus

SubgroupEntamoeba

Supergroup Unikonta


Group Amoebozoa




Parasite amoeba living in the tissue ofinvertebrate and vertebrate animals




Ex. Entamoeba histolytica = causes Amoebicdysentery

SubgroupMyxomycota

Supergroup Unikonta


Group Amoebozoa


aka plasmodial slime molds




-Superficially -true fungi because saprotrophic and produce spores.


-Prominent (feeding/vegetative) stage in the lifecycle exists as a coenocytic (multinucleated) mass of cytoplasm called aplasmodium




When conditions are unfavorable the plasmodiumcan form sporangia that produce geneticallydifferent haploid spores by meiosis




Ex. Physarum

Subgroup Dictyostelida

Supergroup Unikonta


Group Amoebozoa


aka cellular slime molds




-Resemble true fungi saprotrophic andspore production


-Prominent stage of life cycle as individualamoeboid cells that may aggregate to form a pseudoplasmidium


-When conditions are favorable, thepsuedoplasmidium may form sporangia and produce genetically identical haploidspores by mitosis



GroupOpisthokonta

Supergroup Unikonta




Includes all opisthokont (rear flagellated)protists and/or those that evolved from opisthokont ancestors.



SubgroupNucleariida

Supergroup Unikonta


GroupOpisthokonta


aka nucleariids




-Have filose (narrow and tapering)pseudopodia-Have mitochondria with discoid cristae(individual discs)


-Believed to have given rise to kingdom fungi

SubgroupChoanoflagellida

Supergroup Unikonta


GroupOpisthokonta


aka choanoflagellida / collared




-Cells have a membranous collar surrounding thesingle flagellum that used to extract organic material suspended in the watercolumn.




-Believed to have given rise to kingdom Animalia.



Mesophytic dycot leaf

Hydrophytic Leac