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27 Cards in this Set
- Front
- Back
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Cellular respiration – equation and purpose |
C6H12O6+6O2-----6CO2+6H2O+heat Turns Sugar into energy |
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Metabolic Rate |
Rate of Energy Use by an organism Heat Production Mass Specific MR |
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Total Metabolic Rate |
Sugar Molecules divided by organism (Kc/D) |
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Mass Specific Metabolic Rate |
KC/KG/Day |
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Kleiber's Rule |
Animal's Metabolic Rate scales to 3/4s the animal's mass |
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How do volumetric respirometers measure the rate of oxygen consumption? |
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3 Factors that affect metabolic rate |
body size, temperature, and exercise |
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Mitosis |
PMAT |
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Meiosis |
PMATPMAT |
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Plant or Animal Cell |
Plant Cell |
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Plant or Animal Cell |
Animal Cell |
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Chi-Square Formula |
X2=Σ[(o-e)^2/e] o=observed e=expected Σ=Sum |
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Labels on the table |
Observed Expected (Observed-expected) (O-E)^2 (O-E)^2/E |
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Significantly Different |
Less than or equal to .05 percent |
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Monohybrid Cross Dihybrid Cross |
3:1 9:3:3:1 |
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Dihybrid Punnet Square |
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Transformation |
genetic information of an organism is altered by incorporating external genetic material |
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Plasmid |
small circular pieces of DNA |
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What genes did the plasmid in the transformation experiment contain? |
bla, araC, and GFP bla- codes for B-lactamase and gives the bacteria antibiotic resistance araC-promoter gene that turns on GFP GFP-produces a green fluorescent protein |
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What procedures are cells required to go through before transformation? |
By heat shocking the cells, we made them competent and able to suck up the plasmid |
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Which plates should have grown and which should have glowed? |
-pGLO/LB-Growth no glow -pGLO/LB/amp- No growth no glow +pGLO/LB/amp- Growth no glow +pGLO/LB/amp/ara-Grow and Glow |
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What charge is DNA? |
Negatively charged; moves to positive (Runs to Red) |
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Function of restriction enzymes |
Cuts DNA |
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Palindrome Sequence |
Sequences read the same forward as backward |
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How does the size of a DNA band effect how far it moves in the gel? |
The fragment of DNA must weave through the complex matrix created by the agarose gel. It is easier for the smaller bits to do this, so the larger bits don't travel as far. |
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What sugar is used to make the gel? |
agarose |
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Roles of Salt, Soap, and alcohol in DNA extraction |
Salt: Binds to the protein molecules (cheek cells) and draws them out of solution Soap: Pulls apart the lipids and proteins to reveal the DNA Alcohol: Forces the DNA to precipitate |
