Bc: Inhibitors Of Glucose Metabolism

Front 1

Arsenate

Back 1

-Substitutes for Pi in G-3-P Dehydrogenase Rxn of glycolysis.
-Result?

Hint 1

No substrate-level phosphorylation during phosphoglycerate kinase reaction, but reaction still goes forward.

Front 2

Arsenite

Back 2

Allosterically inhibits Pyruvate Dehydrogenase Complex and alpha-ketoglutarate complex, halting TCA cycle.
-Result?

Hint 2

Lethal

Front 3

Heavy Metals & Alkylating Reagents
(Mercury; Iodoacetate)

Back 3

Binds sulfhydryl group in the active site of G-3-P Dehydrogenase.
-Result?

Hint 3

Step 6 inhibition; G-3-P can't be converted to 1,3-BPG, and glycolysis stops.

Front 4

Fluoride

Back 4

Blocks Enolase reaction in glycolysis, preventing production of PEP.
-Used for?

Hint 4

Preserving samples for blood sugar tests.

Front 5

Fluoroacetate

Back 5

Combines with CoA, reacts with citrate synthase to produce fluorocitrate, which binds tightly to aconitase.
-Result?

Hint 5

TCA cycle stops.

Front 6

Malonate

Back 6

Binds active site of succinate dehydrogenase, blocking TCA cycle.
-Other result?

Hint 6

Inhibits Complex II in ETC (succinate dehydrogenase is the acceptor for FADH2)

Front 7

2,4-Dinitrophenol

Back 7

Carries protons across inner membrane of mitochondria, uncoupling ETC from Oxidative Phosphorylation.
-Result?

Hint 7

Rapid consumption of energy without ATP production, which produces excess heat. Body senses ATP deficiency and upregulates energy pathway, causing further heating. Death from overheating can occur.

Front 8

Rotenone

Back 8

Inhibits Complex I of ETC.
-How can ETC continue?

Hint 8

Addition of succinate allows Complex II to continue, w/ less ATP production.

Front 9

Antimycin A

Back 9

Inhibits Complex III of ETC.
-How can ETC continue?

Hint 9

Addition of Ascorbate allows Cyt c to continue, with little ATP production.

Front 10

Sodium Azide

Back 10

Inhibits Complex IV of ETC by binding irreversibly with heme cofactor.
-Particular target organs?

Hint 10

Those that undergo high rates of respiration are affected most, including heart and brain.

Front 11

Carbon Monoxide

Back 11

Inhibits Complex IV of ETC by binding irreversibly with heme cofactor.

Front 12

Cyanide

Back 12

Inhibits Complex IV of ETC.