Bacti - Lab Review

Front 1

A positive result for CAMP test is indicated by ?

Back 1

A positive result is indicated by an "arrowhead"-shaped enhanced zone of b-hemolysis

Front 2

Triple Sugar Iron agar

Which is Salmonella and which is E.coli?

Which examples that might yield these results? proteus, kleibsiella, psuedom.?

Back 2

Which is which?
left: Salmonella (red or purple; not fermenting)
right: E. coli (yellow due to low pH)

fyi: proteus, kleibsiella also produce yellow color with TSI; while pseudomonas retains red color

Front 3

Identify from following information:
Gram + Bacillus
Catalase test Pos.
CAMP +
Glucose fermentation +

Back 3

Corynebacterium renale
Gram + Bacillus
Catalase test Pos.
CAMP +
Glucose fermentation +

Front 4

Identify from following information:
Gram + Bacillus
Catalase test Pos.
CAMP +
Glucose fermentation -

Back 4

Rhodococcus equi
Gram + Bacillus
Catalase test Pos.
CAMP +
Glucose fermentation -

Front 5

Which forms chains strep or staph?

Back 5

Streptococcus

Front 6

Strep or staph?

Back 6

Staphylococcus

Front 7

Which staph have dbl. zone hemolysis?

Back 7

Staphylococcus aureus & S. intermedius

Front 8

Catalase positive strep or staph?

Back 8

Staphylococcus

Catalase test +

Front 9

Staph. which forms non-hemolytic colonies on blood agar

Back 9

Staphylococcus epidermidis – forms non-hemolytic colonies on blood agar

Front 10

type hemolysis?

Back 10

: alpha hemolytic streptococci. (note greening/ incomplete hemolysis)

Front 11

Which Strep. exclusively associated with mammary gland. Recurring mastitis
Catalase negative, mostly B hemolytic, CAMP +ve (arrow-head pattern)

Back 11

S. agalactiae

Front 12

Lactose fermenters (LF) appear what color on plate?
(which plate above?)
Give 2 example of gram negative lactose fermenters:

Back 12

Lactose fermenters (LF) (Pink on Mac)(right)
Escherichia coli, Klebsiella

Front 13

Examples of Non-Lactose fermenters (NLF)

Back 13

Non-Lactose fermenters (NLF)
(no color on MAC)
Salmonella, Proteus
note: these all Gram-ve rods

Front 14

Mucoid colonies on blood agar
Lactose fermenting pink colonies on MacConkey agar

Back 14

Klebsiella pneumoniae: mucoid colonies on blood agar
Lactose fermenting pink colonies on MacConkey agar

Front 15

T/F
E. coli: Gram smear(Klebsiella, Salmonella, Proteus: cannot be distinguished in smear)

Back 15

True

Front 16

E. Coli and Klebsiella inoculated into soft agar ; urease test

Results?

Back 16

E. Coli and Klebsiella inoculated into soft agar (SIM: sulfide-indole-motility medium):
E. coli: motile; urease – negative (clear/yellow)
Klebsiella: non-motile; urease – positive (not shown but should appear red)

Front 17

swarming growth on blood agar plate

Back 17

Proteus mirabilis : swarming growth on blood agar plate

Front 18

Gram - Rod
Non-lactose-fermenter
Most strains produce green pigment
Colorless to greenish colonies on MacConkey medium

Back 18

Pseudomonas aeruginosa : Gram smear (G-ve rods )
Non-lactose-fermenter
Colorless to greenish colonies on MacConkey medium

Front 19

Is Pseudomonas aeruginosa: positive oxidase test?

Back 19

Yes
Pseudomonas aeruginosa: greenish colonies and a positive oxidase test
FYI: some strains hemolytic, some not!

Front 20

Culture : non hemol colonies, no growth- MacConkey
Culture exudates, lung or transtracheal wash
Culture nasal swabs from pigs (atrophic rhinitis), rabbits (snuffles)

Back 20

PASTEURELLOSIS (P.multocida)Coccobacilli

Front 21

(Gram-negative, curvy rods)

hint: incubated in a microaerophilic atmosphere

Back 21

Campylobacter

Front 22

Terminal spores (drumstick)

Back 22

Terminal spores (drumstick)
Tetanus (lockjaw) in animals, humans
Habitat: soil

Front 23

(acid fast stain) on rectal scraping -> Fig: clumps of bacilli (pink)


Johne’s disease: diagnosis

Back 23

Mycobacterium paratuberculosis

Front 24

Unicellular fungi (yeasts)

Back 24

Malassezia in dogs’s ear: Unicellular fungi (yeasts)

Front 25

on Sabouraud agar, 5 days

Back 25

Candida albicans on Sabouraud agar, 5 days

Front 26

spindle shaped macroconidia (lactophenol cotton blue wet mount from culture)

Back 26

Microspoum canis : spindle shaped macroconidia (lactophenol cotton blue wet mount from culture)

Front 27

: boat shaped macroconidia

Back 27

Microsporum gypseum : boat shaped macroconidia

Front 28

wet mount from culture (lactophenol cotton blue stain)

Back 28

Aspergillus fumigatus : wet mount from culture (lactophenol cotton blue stain)

Front 29

What closed sporangium (left)Aseptate hyphae in tissue

Back 29

Zygomycosis Rhizopus : closed sporangium (left)Aseptate hyphae in tissue

Front 30

Gram stain of pus (Gr+ Bacillus)
Catalase Negative
CAMP -
Culture on BA”
Penicillin effective

Back 30

Arcanobacterium pyogenes

Front 31

identify

colonies that tend to become salmon pink on BA

Back 31

Rhodococcus equi (formerly Corynebacterium equi): colonies that tend to become salmon pink

Front 32

identify culture on BA

Gram-positive, catalase-positive, rod-shaped bacteria. It forms partially acid-fast beaded branching filaments (acting as fungi, but being truly bacteria).

Back 32

Nocardia

Gram-positive, catalase-positive, rod-shaped bacteria. It forms partially acid-fast beaded branching filaments (acting as fungi, but being truly bacteria).