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12 Cards in this Set

  • Front
  • Back
3 advantages to molecular methods
1. detect small numbers
2. detect organisms that cant be cultured
3. assays are fast (theretically)
Four disadvantages to amplification methods
1. must have characterized nucleic acid target
2. must interpret the meaning of positive test
3. False positives (contamination)
4. time not always fast
amplification format that involves a unique target.

uses amplification to detect the smallest possible number of target molecules. most sensitive, but may be useful only when detection of any evidence of organism correlates with disease
amplification format that measures abundance of target

(viral load) - use of technique that generates a graded signal that correlates with target number. useful follwoing therapy. may also be useful in trying to sort out endogenous carriage from Disease
4 techniques for amplification
PCR, TMA (transcription mediated amplification), LCR (ligase chain reaction), bDNA (branched chain DNA amplification
specimen type used for TMA diagnosis of chlamydia and Gonorrhea
endocervical, urethral, urine
TMA (transcription mediated amplification) can be used not only to diagnose symptomatic STD but to
screen asymptomatic populations
used to identify isolated colonies in solution
Two techniques to compair strains for epidemiology
1. PFGE (pulsed field gel electrophoresis)
two things used to identify an unknown organism
1. hybridization
2. PCR and sequencing
a low positive predictive value (low PPV) means that
all positives must be confirmed using alternative target
process of Pulsed-Field Gell Electrophoresis
1. rare cutting restriction enzyme to make large fragments of bacterial DNA
2. separte fragments by gell in electrical field
3. create pattern of 10-20 bands
4. compare patterns of organism for relatedness