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7 Cards in this Set
- Front
- Back
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Basic Bacterial Smears |
Individual cell shape (bacillus, coccus) -Arrangement parrerns :Pallisade 0000 |
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Simple (Basic) Staining |
Stains Involved: 1. Crystal Ultraviolet 2. Methylene Blue 3. Carbol Fuchin
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Negative Staining |
Acidic Stain -Nigrosin (stain black) color associated with (-) charge
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Gram Stain |
Gram + : PURPLE (S. aureus) Gram - : PINK (E. Coli)
Primary Stain: 1. Crystal Violet (basic) 2. Gram's Iodine (intensifies Stain) 3. Ethanol (decolorization) 4. Safranin (counter stain) |
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Difference in Basic (Simple) and Negative Stain |
Basic (Simple) had different colors
Negative Stain colors appeared colorless
*Color is associated with Positive Salt molecule -Cells usually have a negative cell wall *Bacteria attracted too (-) cell wall, bacteria is then colored* |
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Gram Smear Technique
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Hear fixation: swiping
Sterilization: killing access bacteria |
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Procedure: Negative vs. Simple |
Negative: -Use the second slide to smear the bacteria across the plate
Simple: -Use a drop of water to place bacteria onto the agar -Spread around the round circle -Hold on top of flame to dry completely, -Swipe three times |
