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16 Cards in this Set
- Front
- Back
CTA Sugar fermentations (). catarrhalis
Color of a positive test |
All negative (glucose, maltose, lactose, sucrose)
DNA ase + yellow + red - |
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Why can't we diagnose Gon/Chlam on direct gram stain in women?
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Acinetobacter looks just like (gram - diplococci)
Part of normal women flora (can in men) |
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Oxidase test in Neisseria and M catarrhalis
How do we transport G/C specimens |
Positive
charcoal swabs |
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Hockey Puck colony
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M. catarrhalis
GN diplococci PNA, eye, sinus, otitis media Resistant to amp by B-lactamase |
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Gram negative cocci
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Neisseria (G and meningitidis), M. catarrhalis
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Gram positive bacilli
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Corynebacterium (diptheriae, jekeium, urealyticum)
Bacillus (anthracis, cereus) Listeria Monocytogenes Erysipelothrix rhusiopathiae |
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c. diptheriae agar for isolation
Test to look for granules |
Black colonies with brown halo on cysteine tellurite (modified Tinsdale)
Loeffler's slant and methylene blue "metachromatic granules" |
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How to find c. diptheriae toxin
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Elek immunoprecipitation procedure
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C. jekeium-claim to fame
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Very resistant to abx (susceptible to vanc and tetracycline)
Plastic catheters, normal skin flora, heart patients |
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General Features of corynebacterium
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diptheroid GPB
No spores catalase + |
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General featues of Bacillus
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Boxcar GPB
Spores Catalase + |
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B. anthracis
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Highly resistant
Black eschar skin lesions, pulm, GI, CNS Medusa head colonies on blood agar Non-hemolytic Non-motile |
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B. cereus
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rapid onset (1-6 hours) post fried rice
B-hemolytic, motile |
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Listeria monocytogenes pathologic features
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B-hemolytic (can look like Group B strep-very small rods)
Catalase + Intracellular pathogen in macs Tumbling motility (more motile at 25 than 35 degrees) Umbrella motility stab + (likes anaerobic part of tube) Grows at 4 degrees |
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Erysipelothrix rhusiopathiae
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Alpha-hemolytic
Catalase - H2S production (only GPR that produces H2S) on TSI skin infection acquired by swine |
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Arcanobacterium hemolyticum
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GPB
B-hemolytic Catalase - Don't confuse with strep |