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16 Cards in this Set

  • Front
  • Back
CTA Sugar fermentations (). catarrhalis
Color of a positive test
All negative (glucose, maltose, lactose, sucrose)
DNA ase +
yellow +
red -
Why can't we diagnose Gon/Chlam on direct gram stain in women?
Acinetobacter looks just like (gram - diplococci)
Part of normal women flora
(can in men)
Oxidase test in Neisseria and M catarrhalis

How do we transport G/C specimens
Positive

charcoal swabs
Hockey Puck colony
M. catarrhalis
GN diplococci
PNA, eye, sinus, otitis media
Resistant to amp by B-lactamase
Gram negative cocci
Neisseria (G and meningitidis), M. catarrhalis
Gram positive bacilli
Corynebacterium (diptheriae, jekeium, urealyticum)
Bacillus (anthracis, cereus)
Listeria Monocytogenes
Erysipelothrix rhusiopathiae
c. diptheriae agar for isolation

Test to look for granules
Black colonies with brown halo on cysteine tellurite (modified Tinsdale)

Loeffler's slant and methylene blue "metachromatic granules"
How to find c. diptheriae toxin
Elek immunoprecipitation procedure
C. jekeium-claim to fame
Very resistant to abx (susceptible to vanc and tetracycline)
Plastic catheters, normal skin flora, heart patients
General Features of corynebacterium
diptheroid GPB
No spores
catalase +
General featues of Bacillus
Boxcar GPB
Spores
Catalase +
B. anthracis
Highly resistant
Black eschar skin lesions, pulm, GI, CNS
Medusa head colonies on blood agar
Non-hemolytic
Non-motile
B. cereus
rapid onset (1-6 hours) post fried rice
B-hemolytic, motile
Listeria monocytogenes pathologic features
B-hemolytic (can look like Group B strep-very small rods)
Catalase +
Intracellular pathogen in macs
Tumbling motility (more motile at 25 than 35 degrees)
Umbrella motility stab + (likes anaerobic part of tube)
Grows at 4 degrees
Erysipelothrix rhusiopathiae
Alpha-hemolytic
Catalase -
H2S production (only GPR that produces H2S) on TSI
skin infection acquired by swine
Arcanobacterium hemolyticum
GPB
B-hemolytic
Catalase -
Don't confuse with strep