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18 Cards in this Set

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  • Back

The generation time

is the time between two successive divisions.

Aerotolerant anaerobes

have an anaerobic pattern of metabolism but can tolerate the presence of oxygen because they possess superoxide dismutase

Capnophilic bacteria

bacteria which require higher concentration of CO2 (5-10%) for growth.

Mesophiles

are organisms able to grow within a temperature range of 20-40*C.

Psychrophiles

(cold-loving) are capable of growth at refrigerator temperature (0-8*C) e.g. Flavobacterium spp.

Thermophiles

(heat-loving) grow best at high temperature (>60*C) e.g. Bacillus stearothermophilus

Growth

It is the increase in number and size of organisms. It can be seen in one of two main forms:


1- Development of colonies, which are the macroscopic products of 20-30 cell divisions of a single bacterium on solid medium.


2- Transformation of a clear fluid medium to a turbid suspension.

Colony

it is the macroscopic product of 20-30 cell divisions of a single bacterium.

Bacterial reproduction

Takes place by simple binary fission :


1- the cell grows in size, usually elongates.


2- The bacterial chromosome acts as a template for the replication of another copy.


3- Each copy becomes attached to a mesosome on the cytoplasmic membrane.


4- The protoplasm becomes divided into two equal parts by the growth of a transverse septum from the cytoplasmic membrane and cell wall.

Classification of bacteria according to O2 requirement:

1- Obligate (strict) aerobe


2- Obligate (strict) anaerobe


3- Facultative anaerobe


4- Micro-aerophilic


5- Aerotolerant anaerobe

Growth Phases (bacterial growth curve) photo
1- Lag phase
2- Log phase (Logarithm/ Exponential)
3- Stationary phase
4- Decline phase

The time needed for development of colonies of M. tuberculosis

generation time= 24 hours


colony= 20-30 times


1*20= 20-30 days

Most bacteria of medical imp are Heterotrophs.

because these bacteria require organic sources of carbon, as the cannot synthesize complex organic substances from simple inorganic sources.

Obligate anaerobes die in the presence of oxygen.

due to accumulation of superoxide and hydrogen peroxide because it lacks the enzymes superoxide dismutase and catalase, which detoxify these molecules.

The net ATP yield in anaerobic respiration is less than it is with aerobic respiration

In anaerobic respiration the final electron acceptor is an inorganic molecule such as nitrate, sulfate or CO2 which are not as good at accepting electrons as oxygen.

The number of viable bacteria is constant during the stationary phase.

because the loss of cells through death is balanced by formation of new cells through growth and division.

The number of viable bacteria decreases during the decline phase.

due to nutrient exhaustion and accumulation of toxic metabolic end products.

The number of bacteria remains constant in the lag phase.

because there is no growth of bacteria which only adapts to the new environment by forming enzymes and intermediates to permit growth.