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14 Cards in this Set
- Front
- Back
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What are the routine media used for sputum samples and what does each do?
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BA (Blood Agar)- non selective medium
BCA (Bacitracin Chocolate Agar)- Bacitracin inhibits most strep, staph, Neisseria and Micrococcus. Used for H.Influenzae (resistant to bacitracin) MAC (MacConkey)- selective G-ve, differentiate lactose fermenters CNA (colistin naladixic acid)- select G+ve. |
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Bacteria found as normal flora in sputum and their characteristics
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Neisseria meningitidis- G-ve cocci, ox+ve
Streptococcus (viridans)- G+ve cocci, cat-ve Haemophilus- G-ve bacilli(BCA agar) Corynebacterium (diphtheroids)- G+ve bacilli, cat+ve Moraxella G-ve cocci Micrococcus- G+ve cocci, cat+ve, ox+ve Staph epidermidis- G+ve cocci, cat+ve, ox-ve, coag+ve. Lactobacillus- G+ve bacilli, cat-ve |
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Pathogenic bacteria in sputum. What disease does it cause?
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H.influenzae- Epiglottitis & Bronchitis
S.pyogenes- Pharyngitis and Tonsillitis Corynebacterium diphtheriae- Diphtheria Bordetella pertussis- Whooping cough S.pneumoniae- Bronchitis and Pneumonia (Typical) Mycobacterium tuberculosis- Tuberculosis Chlamydophilia pneumoniae- Atypical Pneumonia |
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Special media for respiratory bacteria
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N.gonorrhoeae- Modified Thayer-Martin & New York City
C. diphtheriae- Tinsdale Agar (H2S= black colonies) Bordetella pertussis- Bordet-Gengou agar, Charcoal agar, Regan-Lowe agar |
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What are the routine media used for urine samples and what does each do?
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BA
CLED- (cystine lactose electrolyte deficient medium)- prevents proyeus swarming, lactose fermenters MAC (MacConkey)- selective G-ve, lactose fermenters |
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Bacteria that cause UTI's
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E.coli
Staph saprophyticus Enterobacteriaciae (G-ve aerobic bacilli)- Klebsiella, Proteus, Enterobacter Pseudomonas aeruginosa |
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Urinary tract Normal flora
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Coag-ve Staph
viridans and non-haemolytic strep corynebacterium (diphtheroids) Commensal Neisseria, Mycobacterium, Mycoplasma |
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What are the routine media used for Fecal samples and what does each do?
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XLD (Xylose, lysine deoxycholate)- select G-ve, lactose, H2S production. Salmonella, Shigella and E.coli.
CIN (Cefsulodin, Irgasan, Novobiocin)- select G-ve and for yersinea and Aeromonas. CAMP- select for campylobacter (microaerophilic environment @ 42 degrees) TCBS- selects for vibrio Selenite broth- assist growth of salmonella |
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What normal flora grows on XLD that is lactose fermenting?
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E.coli
Aeromonas hydrophilia (also grows on CIN as large flat colonies) |
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Acid fast bacteria in sputum
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Mycobacterium tuberculosis
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Anaerobic bacteria
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Clostridium
Bacteriodes |
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Transport medium for fecal samples
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Carey-Blair
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What are the routine media used for wound swabs and what does each do?
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BA
CNA (colistin naladixic acid)- Select for G+ve MAC (MacConkey)- Select for G-ve ANA- anearobic KV |
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What are the routine media used for genital swabs and what does each do?
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BA
CNA NYC- select for N.gonorrhoeae MAC |
