Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
23 Cards in this Set
- Front
- Back
|
what is automated analysis?
|
mechanical or electrical control of a process that's repetitive,to minimize error and increase efficiency.
|
|
|
what is continuous flow?
|
-Continuous pumping of reagents into 1 rxn chamber;
-Specimens enter segmented flow at intervals. |
|
|
How do automated analyzers meter out sample/reagent?
|
with tubes of various diameters.
|
|
|
what is contained in the stream?
|
-Sample
-Reagent -Bubbles -Water wash between segments. |
|
|
What can interfere with analyte msmt, and how is it removed?
|
Protein - by dialysis.
|
|
|
-How many components are in a continuous flow auto analyzer?
-What are they? |
Seven.
1) Sampler 2) Proportioning pump 3) Manifold 4) Dialyzer 5) Heating bath 6) Colorimeter 7) Recorder |
|
|
What does a sampler do?
|
aspirates samples, standards, and wash solutions in a timed sequence.
|
|
|
what are the parts of a sampler?
|
Cam, motor to turn the cam.
Sample/wash aspirator. |
|
|
What is the heart of the system?
|
Proportioning pump to move sample/reagent/air
|
|
|
what does the manifold do?
|
proportions, mixes, carries fluid through analysis by pump action.
|
|
|
what is the dialyzer for?
|
removing protein and interfering substances.
|
|
|
what factors influence dialysis?
|
-Time, Temp, Membrane type and Membrane surface. ALL CONSTANT.
-Concentration - the only variable. |
|
|
How is the heating bath constructed,and why?
|
So that solution flows from bottom up - gives time delay for ample color development.
|
|
|
What happens to the stream before color measurment?
|
DEBUBBLING.
|
|
|
how does sample go through the colorimeter?
|
through a flow cell.
|
|
|
what 4 components are in the optical colorimeter for auto analysis?
|
1. Tungsten lamp
2. Interference FILTER 3. 2 photocells - double beam in space. 4. Null balance circuit. |
|
|
what type of recorder is used?
|
a pen that draws a graph corresponding to color measurement. will reflect bubbles if present!
|
|
|
what other photodetectors could be used?
|
-Flame photometer
-Fluorometer |
|
|
what is DISCRETE ANALYSIS?
|
chemistry analysis of samples SEPERATED BY PHYSICAL BARRIERS.
|
|
|
what is commonly used to seperate the samples?
|
plastic envelopes with reagents in them.
|
|
|
what is the Ektachem currently called?
|
the Vitros
|
|
|
what type of analysis does the Vitros use?
|
-Dry slide technology with ion selective electrodes.
|
|
|
what are the 4 key concepts re: the vitros?
|
-Liquid free(dryslide) technol.
-Film slide -Reflectance spectrophotometry for opaque slides/samples -Air displacement sampling. |
