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5 Cards in this Set
- Front
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Use of aseptic techniquez |
Keeps apparatus and equipment free of micro organisms Used to prevent contamination of bacterial cultures and surrounding environment |
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Prevent contamination of cultures and apparatus from environment |
Sterilise all apparatus and media before use Handle cultures carefully Use equipment e.g sterile loop to prevent subsequent contamination |
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Prevent contaminationto the environment by the bateria being used experiements |
Sterilise the work surface before and after experiement using disinfectant Use the correct handling techniques to prevent contamination of personnel and environment by organisms being cultured |
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Examples of preventing contamination to the environment |
Carrying out the process of inoculations Grasp the culture bottle in 1 hand and flame the mouth for 2 to 3 seconds Pass loop through flame Lift lid 45° Secure lid with tapd in 4 places to allow aerobic conditions (prevents pathogenic growth) Incubate at 25°c Dont open |
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Lab sterilisation |
Use an autoclave -sealed containerin which gas and ßp |
