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21 Cards in this Set
- Front
- Back
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Describe the ONPG test |
Substrate - ONPG, IPTG Reaction/Enzymes - beta galactosidase Negative - colourless Positive - yellow |
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Describe the ADH test |
Substrate - Arginine Reaction/Enzyme - dihydrolase Negative - yellow Positive - red/orange |
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Describe the LDC test |
Substrate - lysine Reaction/Enzyme - decarboxylase Negative - yellow Positive - orange/red |
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Describe the ODC test |
Substrate - ornathine Reaction - decarboxylase Negative - yellow Positive - red/orange |
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Describe the CIT test |
Substrate - sodium citrate Reaction - citrate utilisation Negative - pale green/yellow Positive - blue |
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Describe the H2S test |
Substrate - sodium thiosulphate Reaction - H2S production Negative - colourless/greyish Positive - black deposits/thin line |
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Describe the URE test |
Substrate - urea Reaction - urease Negative - yellow Positive - red/orange |
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Describe the TDA test |
Substrate - tryptophane Reaction - deaminase Positive - TDA intermediate- reddish brown Negative - no colour change |
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Describe the IND test |
Substrate - tryptophane Reaction - indole production Positive - JAMES, pink Negative - no colour change |
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Describe the VP test |
Substrate - creatine sodium pyruvate Reaction - acetoin production Positive - VP1+VP2 - pink/red Negative - slightly pink |
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Describe the GEL test |
Substrate - Kohns gelatine Reaction - gelatinase Positive - diffusion of black pigment Negative - no diffusion |
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Describe the GLU test |
Substrate - glucose Reaction - fermentation/oxidation Negative - blue/blue green Positive - yellow/greyish yellow |
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Describe the MAN test |
Substrate - mannitol Reaction - fermentation/oxidation Negative - blue/blue green Positive - yellow |
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Describe the INO test |
Substrate - inositol Reaction - fermentation/oxidation Negative - blue/blue green Positive - yellow |
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Describe the SOR test |
Substrate - sorbitol Reaction - fermentation/oxidation Negative - blue/blue green Positive - yellow |
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Describe the RHA test |
Substrate - rhamnose Reaction - fermentation/oxidation Negative - blue/blue green Positive - yellow |
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Describe the SAC test |
Substrate - sucrose Reaction - fermentation/oxidation Negative - blue/blue green Positive - yellow |
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Describe the MEL test |
Substrate - melibiose Reaction - fermentation/oxidation Negative - blue/blue green Positive - yellow |
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Describe the AMY test |
Substrate - amygdaiin Reaction - fermentation/oxidation Negative - blue/blue green Positive - yellow |
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Describe the ARA test |
Substrate - arabinose Reaction - fermentation/oxidation Negative - blue/blue green Positive - yellow |
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Describe the OX test |
Substrate - cytochrome C oxidises electron receptor Reaction - cytochrome oxidase Positive for certain organisms |
