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55 Cards in this Set

  • Front
  • Back
transcription
making mRNA from DNA
Translation
making a protein from mRNA
RNA polymerase
the enzyme used to make RNA from DNA once the DNA unwinds.
transcribed DNA strand
protein making side
untranscribed DNA strand
the quiet side of the DNA. its not being transcribed at the moment.
RNA ploymerase 1
only transcribes rRNA genes - makes ribosomes
RNA polymerase 2
transcribes genes into mRNA
RNA polymerase 3
transcribes tRNA genes
Promoter
the binding site before the begining of a gene. its the binding site for RNA polymerase and transcription factors
Tata box
the starting point of the promoter
enhancer region
the binding site thats far upstream of the gene, it quickens the transcription process.
exons
the genes that are expressed
introns
the junk in between the real dna
mRNA splicing
when the introns are removed from the mRNA and just the exons are left
snRNP
small nuclear RNA
spliceosome
several snRNPs. it gets rid of the intron.
alternative splicing
while trying to make two different proteins, some genes are considered introns for one, and they are considered exons for another.
codon
the 3 letters that are paired on the mRNA (ex AUG, CGU, etc)
anticodon
the 3 letters that are on the tRNA that code for an amino acid
aminoacyl tRNA synthetase
the enzyme that bonds the amino acid to the tRNA
ribosome A site
holds the tRNA that is carrying the next amino actid to be added to the chain
ribosome P site
holds the tRNA that is carrying the growing polypeptide chain
ribosome E site
the empty tRNA leaves the ribosome from this exit site
helicase
the enzyme that unwinds part of the DNA helix
single-stranded binding proteins
keeps the DNA open as it unzips
RNA primer
in order to make new DNA strand, there must be something to start it with, so RNA primer brings in a little piece of RNA to get started.
DNA polymerase 1
gets rid of tiny bit of RNA and replaces it with DNA nucleotides
DNA polymerase 2
starts at the small piece of RNA and adds DNA nucleotides to make new strand.
okazaki fragments
the small pieces of DNA that have to skip. this is because it can only go from 5' to 3'. so if it is opposite, it must skip.
leading strand
can smoothly go from 5' to 3' cuz it can keep attatching to the 3 carbon
lagging strand
the strand that goes from 3' to 5' so it must skip
bacteriophage
a virus that infects bacteria
restriction enzymes
enzymes that "cut" the DNA when they find an order of nucleotides
ligase
an enzyme that "seals" strands of DNA together
plasmids
small, self replicating circular DNA molecules
polymerase chain reaction
a method for making many copies of a specifig segment of DNA. (with only using one cell, someone can make tons of DNA to do tests on)
reproductive cloning
when you take the nucleus out of a somatic cell and put it into an enucleated egg cell. (dolly the sheep)
therapeutic cloning
dna into stem cells in order to grow something someone needs (ex: new kidney)
alosteric inhibitor
a product goes in an binds to the alosteric site of an enzyme to stop production of said product.
operon
a group of genes taht have related functions.
operator
DNA binding site of a repressor protein
repressor protein
binds to the DNA at the operator site. it blocks RNA polymerase and it blocks transcription
repressible operon : tryptophan
when trp is present in excess amounts, it binds tot he repressor protein and it triggers the repressor to bind to the DNA, causing the RNA polymerase to not be able to bind and it wont make anymore trp.
inducible operon : lactose
when lac is present, it binds the lac repressor protein and it makes it release from the DNA, making the RNA polymerase to bind and create enzymes to digest lac.
epigenetics
an external molecule that can control DNA
siRNA
small interfereing RNA - short segments of RNA that code for a "death" tag for mRNA. it turns off the gene so that there is no protein produced.
ubiquitin
a death tag label for unwanted proteins
blastocyst
a 5 to 6 day fertilized egg - used for stem cells
point mutations
a single base change in the DNA
silent mutation
a mutation that does not change the amino acid produced
missense
a change in an amino acid due to a point mutation
nonsense
a change in the stop codon due to point mutations
frameshift
a mutation that shifts everything downstream of the DNA
insertions
adding a base
deletions
losing bases