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35 Cards in this Set
- Front
- Back
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What is serum?
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The fluid phase that remains in clotted blood or plasma.
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What part of blood do antibodies reside?
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the Serum
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How heavy is IgG normally?
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150,000 Da.
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Digestion of IgG with Papain produces:
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3 fragments: 2 Fab, 1 Fc
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Digestion of IgG with Pepsin produces:
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1 F(ab')2.
Fc is digested into pieces |
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What does mercaptoethanol reduction of the disulfide bonds in IgG show?
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That there are 2 heavy chains and 2 light chains.
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how much do the light and heavy chains of IgG weigh?
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Heavy = 50 kDa
Light = 25 kDa |
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What is multiple myeloma?
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Unregulated proliferation of plasma cells that produce tons of homogenous antibody.
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What are bence-jones proteins?
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extra light chains secreted by myeloma cells in cancer patients.
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How many types of light chains are there? What are they?
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2; Kappa and Lambda
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How many types of Heavy chains are there, what are they?
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5;
IgG, IgM, IgD, IgA, IgE |
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Which isotypes have subclasses and how many?
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IgG - 4 subclasses
IgA - 2 subclasses |
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What is the molecular unit of an antibody?
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an Immunoglobulin domain with an intramolecular disulfide bond.
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Characteristics of the Ig Domain:
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100 residues
1 intramolecular disulfide bond (60 residues per loop) THE BUILDING BLOCK OF THE ANTIBODY |
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What type of structure are Ig domains?
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2ndary structure = beta pleated sheets.
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How many CDRs are in
-each variable domain -each antibody |
3 per domain, so 6 per antibody.
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What does each immunoglob domain consist of?
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2 B-sheets stabilized by a disulfide bond. (from her notes)
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What, structurally, are the variable regions of Ig domains?
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3 Loops between beta sheets that show considerable variation in A.A. sequence.
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which contributes more to antigen binding usually, heavy or light variable regions?
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heavy
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4 Functions of Immunoglobulins:
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1. Precipitation/neutralization
2. Activation of complement 3. Activation of ADCC by NK cells 4. Opsonization for incr. phagocytosis |
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Which antibodies can exist as multimers?
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IgA and IgM
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Which is the most abundant Ab in serum?
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IgG
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Which antibodies activate complement?
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IgG, IgM
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Which Ab cross the placenta?
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IgG
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Which antibody is an opsonin?
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IgG
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which cell is IgM expressed on as a membrane molecule?
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mature B cells
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How does IgM exist in serum?
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Pentamer
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Which Abs have the J chain
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IgM, IgA
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Predominant Ab in mucosal secretions and breast milk:
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IgA
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Ab responsible for allergy:
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IgE
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Which are the first-secreted antibodies in an immune response?
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IgM
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which Ab are present on the mature B cell membrane?
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IgM, IgD
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3 types of Antigenic Determinants on Ab:
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1. Isotypic (what class of Ab it is)
2. Allotypic (diffnces btwn species) 3. Idiotypic (diffnces of Variable regions) |
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2 Ab cross mother's epithelium to enter breast milk:
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IgM, IgA
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How/when is Secretory IgA formed?
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When: during transport through mucous membrane epithelial cells.
How: Dimer of IgA binds a "poly-Ig receptor" on basolateral membrane; epithelial cell endocytoses it; Transport to endothelial surface; cleavage of IgA with secretory component |
